Mouse F13B (Coagulation Factor XIII B Polypeptide) ELISA Kit | MyBioSource MBS2500743 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Mouse F13B (Coagulation Factor XIII B Polypeptide) ELISA Kit

catalog :

MBS2500743

quantity :

48-Strip-Wells

price :

410 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS2500743

products type :

ELISA Kit

products full name :

Mouse F13B (Coagulation Factor XIII B Polypeptide) ELISA Kit

products short name :

F13B

other names :

Fibronectin; Fibronectin; fibronectin; cold-insoluble globulin; migration-stimulating factor; fibronectin 1; Cold-insoluble globulin; CIG

products gene name :

F13B

other gene names :

FN1; FN1; FN; CIG; FNZ; MSF; ED-B; FINC; GFND; LETS; GFND2; FN; FN; CIG

uniprot entry name :

FINC_HUMAN

reactivity :

Mouse

sequence length :

2386

specificity :

This kit recognizes natural and recombinant Mouse F13B. No significant cross-reactivity or interference between Mouse F13B and analogues was observed.

storage stability :

Store at 4 degree C.

other info1 :

Samples: Serum, plasma and other biological fluids. Assay Type: Sandwich. Detection Range: 3.125-200ng/mL. Sensitivity: The minimum detectable dose of Mouse F13B is 1.875ng/mL (The sensitivity of this assay, or lowest detectable limit (LDL) was defined as the lowest protein concentration that could be differentiated from zero).

other info2 :

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Mouse F13B concentrations in serum, plasma and other biological fluids.

products description :

Principle of the assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to F13B. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for F13B and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain F13B, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of F13B. You can calculate the concentration of F13B in the samples by comparing the OD of the samples to the standard curve.

ncbi gi num :

300669710

ncbi acc num :

P02751.4

uniprot acc num :

P02751

ncbi mol weight :

256,502 Da

ncbi pathways :

Amoebiasis Pathway (167324); Amoebiasis Pathway (167191); Angiopoietin Receptor Tie2-mediated Signaling Pathway (137917); Bacterial Invasion Of Epithelial Cells Pathway (149807); Bacterial Invasion Of Epithelial Cells Pathway (148661); Cell Surface Interactions At The Vascular Wall Pathway (106062); ECM-receptor Interaction Pathway (83068); ECM-receptor Interaction Pathway (479); Extracellular Matrix Organization Pathway (576262); Fibronectin Matrix Formation Pathway (771601)

ncbi summary :

This gene encodes fibronectin, a glycoprotein present in a soluble dimeric form in plasma, and in a dimeric or multimeric form at the cell surface and in extracellular matrix. Fibronectin is involved in cell adhesion and migration processes including embryogenesis, wound healing, blood coagulation, host defense, and metastasis. The gene has three regions subject to alternative splicing, with the potential to produce 20 different transcript variants. However, the full-length nature of some variants has not been determined. [provided by RefSeq, Jul 2008]

uniprot summary :

FN1: Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin. Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape. Defects in FN1 are the cause of glomerulopathy with fibronectin deposits type 2 (GFND2); also known as familial glomerular nephritis with fibronectin deposits or fibronectin glomerulopathy. GFND is a genetically heterogeneous autosomal dominant disorder characterized clinically by proteinuria, microscopic hematuria, and hypertension that leads to end-stage renal failure in the second to fifth decade of life. 15 isoforms of the human protein are produced by alternative splicing. Protein type: Motility/polarity/chemotaxis; Secreted, signal peptide; Secreted; Cell adhesion. Chromosomal Location of Human Ortholog: 2q34. Cellular Component: extracellular matrix; extracellular space; fibrinogen complex; apical plasma membrane; extracellular region; ER-Golgi intermediate compartment; basal lamina. Molecular Function: collagen binding; integrin binding; heparin binding; protein binding; protease activator activity; protease binding. Biological Process: integrin activation; platelet activation; extracellular matrix organization and biogenesis; positive regulation of axon extension; extracellular matrix disassembly; regulation of cell shape; platelet degranulation; calcium-independent cell-matrix adhesion; response to wounding; acute-phase response; cell-substrate junction assembly; peptide cross-linking; angiogenesis; cell adhesion; blood coagulation; leukocyte migration. Disease: Glomerulopathy With Fibronectin Deposits 2; Plasma Fibronectin Deficiency

size1 :

48-Strip-Wells

price1 :

410 USD

size2 :

96-Strip-Wells

price2 :

490

more info or order :

MyBioSource product webpage