catalog number :
MBS2500679
products type :
ELISA Kit
products full name :
Human S100A12 (S100 Calcium Binding Protein A12) ELISA Kit
products short name :
S100A12
other names :
S100A12; S100A12; S100A12
products gene name :
S100A12
uniprot entry name :
E3UTY8_FELCA
specificity :
This kit recognizes natural and recombinant Human S100A12. No significant cross-reactivity or interference between Human S100A12 and analogues was observed.
storage stability :
Store at 4 degree C.
other info1 :
Samples: Serum, plasma and other biological fluids. Assay Type: Sandwich. Detection Range: 0.156-10ng/mL. Sensitivity: The minimum detectable dose of Human S100A12 is 0.094ng/mL (The sensitivity of this assay, or lowest detectable limit (LDL) was defined as the lowest protein concentration that could be differentiated from zero).
other info2 :
Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Human S100A12 concentrations in serum, plasma and other biological fluids.
products description :
Principle of the assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to S100A12. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for S100A12 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain S100A12, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of S100A12. You can calculate the concentration of S100A12 in the samples by comparing the OD of the samples to the standard curve.
ncbi acc num :
ADP23913.1
ncbi mol weight :
10,700 Da