ELISA/assay

Rat IL-6R (Interleukin 6 Receptor) ELISA Kit

MBS2500665

24-Strip-Wells

215 USD

ELISA Kit

Rat IL-6R (Interleukin 6 Receptor) ELISA Kit

[IL-6R]

[interleukin-6 receptor subunit alpha isoform 2; Interleukin-6 receptor subunit alpha; interleukin-6 receptor subunit alpha; CD126 antigen; IL-6 receptor subunit alpha; IL-6R 1; membrane glycoprotein 80; interleukin 6 receptor; IL-6R 1; Membrane glycoprotein 80; gp80; CD_antigen: CD126]

[IL-6R]

[IL6R; IL6R; IL6Q; gp80; CD126; IL6RA; IL6RQ; IL-6RA; IL-6R-1; IL-6 receptor subunit alpha; IL-6R subunit alpha; IL-6R-alpha; IL-6RA; gp80]

IL6RA_HUMAN

Rat

365

This kit recognizes Rat IL-6 in samples. No significant cross-reactivity or interference between Rat IL-6 and analogues was observed.

Store at 4 degree C.

Typical Testing Data/Standard Curve (for reference only)

Samples: Serum, Plasma And Other Biological Fluids. Assay Type: Quantitative Sandwich. Detection Range: 12.5-800pg/mL. Sensitivity: 7.5pg/mL.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Rat IL-6 were tested 20 times on one plate, respectively. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Rat IL-6 were tested on 3 different plates, 20 replicates in each plate.

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Rat IL-6 concentrations in serum, plasma and other biological fluids. Principle of the Assay: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat IL-6. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat IL-6 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat IL-6, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Rat IL-6. You can calculate the concentration of Rat IL-6 in the samples by comparing the OD of the samples to the standard curve.

31317249

NP_852004.1

NM_181359.2

P08887

40,237 Da

ARMS-mediated Activation Pathway (106466); Activated TLR4 Signalling Pathway (106400); Axon Guidance Pathway (105688); Cytokine Signaling In Immune System Pathway (366171); Cytokine-cytokine Receptor Interaction Pathway (83051); Cytokine-cytokine Receptor Interaction Pathway (460); DAP12 Interactions Pathway (685549); DAP12 Signaling Pathway (685550); Developmental Biology Pathway (477129); Disease Pathway (530764)

This gene encodes a subunit of the interleukin 6 (IL6) receptor complex. Interleukin 6 is a potent pleiotropic cytokine that regulates cell growth and differentiation and plays an important role in the immune response. The IL6 receptor is a protein complex consisting of this protein and interleukin 6 signal transducer (IL6ST/GP130/IL6-beta), a receptor subunit also shared by many other cytokines. Dysregulated production of IL6 and this receptor are implicated in the pathogenesis of many diseases, such as multiple myeloma, autoimmune diseases and prostate cancer. Alternatively spliced transcript variants encoding distinct isoforms have been reported. A pseudogene of this gene is found on chromosome 9.[provided by RefSeq, May 2011]

IL6R: Part of the receptor for interleukin 6. Binds to IL6 with low affinity, but does not transduce a signal. Signal activation necessitate an association with IL6ST. Activation may lead to the regulation of the immune response, acute-phase reactions and hematopoiesis. Belongs to the type I cytokine receptor family. Type 3 subfamily. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Membrane protein, integral; Receptor, cytokine. Chromosomal Location of Human Ortholog: 1q21. Cellular Component: extracellular space; basolateral plasma membrane; apical plasma membrane; plasma membrane; extracellular region; interleukin-6 receptor complex. Molecular Function: protein binding; interleukin-6 binding; enzyme binding; protein homodimerization activity; interleukin-6 receptor binding; ciliary neurotrophic factor receptor activity; interleukin-6 receptor activity. Biological Process: cytokine and chemokine mediated signaling pathway; negative regulation of collagen biosynthetic process; positive regulation of smooth muscle cell proliferation; neutrophil mediated immunity; positive regulation of interleukin-6 production; positive regulation of leukocyte chemotaxis; defense response to Gram-negative bacterium; endocrine pancreas development; positive regulation of chemokine production; activation of NF-kappaB transcription factor; positive regulation of tyrosine phosphorylation of Stat3 protein; positive regulation of osteoblast differentiation; monocyte chemotaxis; defense response to Gram-positive bacterium; positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of MAPKKK cascade; response to cytokine stimulus; positive regulation of cell proliferation; negative regulation of interleukin-8 production; acute-phase response; hepatic immune response. Disease: Interleukin 6, Serum Level Of, Quantitative Trait Locus; Soluble Interleukin-6 Receptor, Serum Level Of, Quantitative Trait Locus

24-Strip-Wells

215 USD

48-Strip-Wells

410

96-Strip-Wells

490

5x96-Strip-Wells

2040

10x96-Strip-Wells

3590