catalog number :
MBS2500602
products type :
ELISA Kit
products full name :
Human ISG15 (Interferon Stimulated Gene 15) ELISA Kit
products short name :
[ISG15]
other names :
[ISG15; ISG15; ISG15]
products gene name :
[ISG15]
uniprot entry name :
M1RFN1_9CETA
specificity :
This kit recognizes Human IL4I1 in samples. No significant cross-reactivity or interference between HumanIL4I1 and analogues was observed.
storage stability :
Store at 4 degree C.
image1 heading :
Typical Testing Data/Standard Curve (for reference only)
other info1 :
Samples: Serum, Plasma And Other Biological Fluids. Assay Type: Quantitative Sandwich. Detection Range: 0.16-10ng/mL. Sensitivity: 0.1ng/mL.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, midrange and high level HumanIL4I1 were tested 20times on one plate, respectively. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, midrange and high level HumanIL4I1 were testedon 3 different plates, 20replicates in each plate.
products description :
Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Human IL4I1 concentrations in serum, plasma and other biological fluids. Principle of the Assay: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human IL4I1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human IL4I1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human IL4I1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human IL4I1. You can calculate the concentration of Human IL4I1 in the samples by comparing the OD of the samples to the standard curve.
ncbi acc num :
AGG15966.1
ncbi mol weight :
17,472 Da
size5 :
10x96-Strip-Wells