catalog number :
MBS2500565
products type :
ELISA Kit
products full name :
Human LDHB (Lactate Dehydrogenase B) ELISA Kit
products short name :
[LDHB]
other names :
[L-lactate dehydrogenase B chain; L-lactate dehydrogenase B chain; L-lactate dehydrogenase B chain; LDH heart subunit; epididymis secretory protein Li 281; lactate dehydrogenase H chain; renal carcinoma antigen NY-REN-46; lactate dehydrogenase B; LDH heart subunit; LDH-H; Renal carcinoma antigen NY-REN-46]
products gene name :
[LDHB]
other gene names :
[LDHB; LDHB; LDH-B; LDH-H; LDHBD; TRG-5; HEL-S-281; LDH-B; LDH-H]
uniprot entry name :
LDHB_HUMAN
specificity :
This kit recognizes natural and recombinant Human LDHB. No significant cross-reactivity or interference between Human LDHB and analogues was observed.
storage stability :
Store at 4 degree C.
image1 heading :
Typical Testing Data/Standard Curve (for reference only)
other info1 :
Samples: Serum, Plasma, Biological Fluids. Assay Type: Sandwich. Detection Range: 3.125-200ng/mL. Sensitivity: Min: 1.875ng/mL; Max: 200ng/mL
products description :
Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Human LDHB concentrations in serum, plasma and other biological fluids. Principle of the Assay This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to LDHB. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for LDHB and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain LDHB, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of LDHB. You can calculate the concentration of LDHB in the samples by comparing the OD of the samples to the standard curve.
ncbi acc num :
NP_002291.1
ncbi gb acc num :
NM_002300.6
ncbi mol weight :
36,638 Da
ncbi pathways :
Abnormal Metabolism In Phenylketonuria Pathway (645287); Cysteine And Methionine Metabolism Pathway (104488); Cysteine And Methionine Metabolism Pathway (103421); Disease Pathway (530764); Glycolysis / Gluconeogenesis Pathway (82926); Glycolysis / Gluconeogenesis Pathway (287); Glycolysis And Gluconeogenesis Pathway (198814); Metabolic Pathways (132956); Metabolism Pathway (477135); Propanoate Metabolism Pathway (83004)
ncbi summary :
This gene encodes an enzyme which catalyzes the reversible conversion of lactate and pyruvate, and NAD and NADH, in the glycolytic pathway. Mutations in this gene are associated with lactate dehydrogenase B deficiency. Pseudogenes have been identified on the X chromosome and on chromosome. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Mar 2010]
size5 :
10x96-Strip-Wells