Human PSA/KLK3 (Prostate Specific Antigen) ELISA Kit | MyBioSource MBS2500373 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human PSA/KLK3 (Prostate Specific Antigen) ELISA Kit

catalog :

MBS2500373

quantity :

48-Strip-Wells

price :

410 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS2500373

products type :

ELISA Kit

products full name :

Human PSA/KLK3 (Prostate Specific Antigen) ELISA Kit

products short name :

PSA/KLK3

other names :

prostate-specific antigen isoform 1 preproprotein; Prostate-specific antigen; prostate-specific antigen; P-30 antigen; gamma-seminoprotein; kallikrein-3; prostate specific antigen; semenogelase; seminin; kallikrein-related peptidase 3; Gamma-seminoprotein; Seminin; Kallikrein-3; P-30 antigen; Semenogelase

products gene name :

PSA/KLK3

other gene names :

KLK3; KLK3; APS; PSA; hK3; KLK2A1; APS; PSA; Seminin

uniprot entry name :

KLK3_HUMAN

reactivity :

Human

sequence length :

261

specificity :

This kit recognizes natural and recombinant Human PSA/KLK3. No significant cross-reactivity or interference between Human PSA/KLK3 and analogues was observed.

storage stability :

Store at 4 degree C.

other info1 :

Samples: Serum, plasma and other biological fluids. Assay Type: Sandwich. Detection Range: 0.938-60ng/mL. Sensitivity: The minimum detectable dose of Human PSA/KLK3 is 0.563ng/mL (The sensitivity of this assay, or lowest detectable limit (LDL) was defined as the lowest protein concentration that could be differentiated from zero).

other info2 :

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Human PSA/KLK3 concentrations in serum, plasma and other biological fluids.

products description :

Principle of the assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to PSA/KLK3. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for PSA/KLK3 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain PSA/KLK3, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of PSA/KLK3. You can calculate the concentration of PSA/KLK3 in the samples by comparing the OD of the samples to the standard curve.

ncbi gi num :

4502173

ncbi acc num :

NP_001639.1

ncbi gb acc num :

NM_001648.2

uniprot acc num :

P07288

ncbi mol weight :

25,116 Da

ncbi pathways :

Androgen Receptor Signaling Pathway (198806); Coregulation Of Androgen Receptor Activity Pathway (138085); FOXA1 Transcription Factor Network Pathway (137979); Metabolism Of Proteins Pathway (106230); Pathways In Cancer (83105); Prostate Cancer Pathway (755440); Prostate Cancer Pathway (83111); Prostate Cancer Pathway (523); Regulation Of Androgen Receptor Activity Pathway (138027); Regulation Of Insulin-like Growth Factor (IGF) Transport And Uptake By Insulin-like Growth Factor Binding Proteins (IGFBPs) Pathway (105908)

ncbi summary :

Kallikreins are a subgroup of serine proteases having diverse physiological functions. Growing evidence suggests that many kallikreins are implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers. This gene is one of the fifteen kallikrein subfamily members located in a cluster on chromosome 19. Its protein product is a protease present in seminal plasma. It is thought to function normally in the liquefaction of seminal coagulum, presumably by hydrolysis of the high molecular mass seminal vesicle protein. Serum level of this protein, called PSA in the clinical setting, is useful in the diagnosis and monitoring of prostatic carcinoma. Alternate splicing of this gene generates several transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]

uniprot summary :

PSA: Hydrolyzes semenogelin-1 thus leading to the liquefaction of the seminal coagulum. Forms heterodimer with SERPINA5. Inhibited by SERPINA5. Activity is strongly inhibited by Zn2+, 100 times more abundant in semen than in serum. This inhibition is relieved by exposure to semenogelins, which are avid zinc binders. Belongs to the peptidase S1 family. Kallikrein subfamily. Protein type: EC 3.4.21.77; Secreted, signal peptide; Protease; Secreted. Chromosomal Location of Human Ortholog: 19q13.41. Cellular Component: extracellular region; nucleus. Molecular Function: protein binding; serine-type peptidase activity; serine-type endopeptidase activity. Biological Process: negative regulation of angiogenesis; cellular protein metabolic process; proteolysis

size1 :

48-Strip-Wells

price1 :

410 USD

size2 :

96-Strip-Wells

price2 :

490

more info or order :

MyBioSource product webpage