ELISA/assay

Human EFNA5 (Ephrin A5) ELISA Kit

MBS2500291

24-Strip-Wells

215 USD

ELISA Kit

Human EFNA5 (Ephrin A5) ELISA Kit

[EFNA5]

[ephrin-A5; Ephrin-A5; ephrin-A5; AL-1; LERK-7; eph-related receptor tyrosine kinase ligand 7; ephrin-A5; AL-1; EPH-related receptor tyrosine kinase ligand 7; LERK-7]

[EFNA5]

[EFNA5; EFNA5; AF1; EFL5; RAGS; EPLG7; GLC1M; LERK7; EPLG7; LERK7; LERK-7]

EFNA5_HUMAN

Human

228

This kit recognizes Human DNMT1 in samples. No significant cross-reactivity or interference between HumanDNMT1and analogues was observed.

Store at 4 degree C.

Typical Testing Data/Standard Curve (for reference only)

Samples: Serum, Plasma And Other Biological Fluids. Assay Type: Quantitative Sandwich. Detection Range: 0.16-10ng/mL. Sensitivity: 0.1ng/mL.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, midrange and high level HumanDNMT1 were tested 20 times on one plate, respectively. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, midrange and high level HumanDNMT1 were testedon 3 different plates, 20 replicates in each plate.

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Human DNMT1 concentrations in serum, plasma and other biological fluids. Principle of the Assay: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human DNMT1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human DNMT1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human DNMT1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human DNMT1. You can calculate the concentration of Human DNMT1 in the samples by comparing the OD of the samples to the standard curve.

4503487

NP_001953.1

NM_001962.2

P52803

26,297 Da

Axon Guidance Pathway (83065); Axon Guidance Pathway (476); Axon Guidance Pathway (105688); Developmental Biology Pathway (477129); EPH-Ephrin Signaling Pathway (1127691); EPH-ephrin Mediated Repulsion Of Cells Pathway (1127695); EPHA Forward Signaling Pathway (138041); EPHA-mediated Growth Cone Collapse Pathway (1127692); EPHB Forward Signaling Pathway (138047); Ephrin A Reverse Signaling Pathway (137986)

Ephrin-A5, a member of the ephrin gene family, prevents axon bundling in cocultures of cortical neurons with astrocytes, a model of late stage nervous system development and differentiation. The EPH and EPH-related receptors comprise the largest subfamily of receptor protein-tyrosine kinases and have been implicated in mediating developmental events, particularly in the nervous system. EPH receptors typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin ligands and receptors have been named by the Eph Nomenclature Committee (1997). Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. The Eph family of receptors are similarly divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. [provided by RefSeq, Jul 2008]

EFNA5: Cell surface GPI-bound ligand for Eph receptors, a family of receptor tyrosine kinases which are crucial for migration, repulsion and adhesion during neuronal, vascular and epithelial development. Binds promiscuously Eph receptors residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Induces compartmentalized signaling within a caveolae-like membrane microdomain when bound to the extracellular domain of its cognate receptor. This signaling event requires the activity of the Fyn tyrosine kinase. Activates the EPHA3 receptor to regulate cell-cell adhesion and cytoskeletal organization. With the receptor EPHA2 may regulate lens fiber cells shape and interactions and be important for lens transparency maintenance. May function actively to stimulate axon fasciculation. The interaction of EFNA5 with EPHA5 also mediates communication between pancreatic islet cells to regulate glucose-stimulated insulin secretion. Cognate/functional ligand for EPHA7, their interaction regulates brain development modulating cell-cell adhesion and repulsion. Belongs to the ephrin family. Protein type: Membrane protein, GPI anchor; Motility/polarity/chemotaxis; Ligand, receptor tyrosine kinase; Cell development/differentiation. Chromosomal Location of Human Ortholog: 5q21. Cellular Component: anchored to external side of plasma membrane; extracellular region; plasma membrane; caveola. Molecular Function: ephrin receptor binding; chemorepellent activity. Biological Process: nervous system development; axon guidance; positive regulation of peptidyl-tyrosine phosphorylation; regulation of cell-cell adhesion; apoptosis; regulation of actin cytoskeleton organization and biogenesis; regulation of focal adhesion formation; ephrin receptor signaling pathway; negative chemotaxis; retinal ganglion cell axon guidance

24-Strip-Wells

215 USD

48-Strip-Wells

410

96-Strip-Wells

490

5x96-Strip-Wells

2040

10x96-Strip-Wells

3590