Human PPAR-delta (Peroxisome Proliferator Activated Receptor Delta) ELISA Kit | MyBioSource MBS2500283 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human PPAR-delta (Peroxisome Proliferator Activated Receptor Delta) ELISA Kit

catalog :

MBS2500283

quantity :

24-Strip-Wells

price :

215 USD

product information

catalog number :

MBS2500283

products type :

ELISA Kit

products full name :

Human PPAR-delta (Peroxisome Proliferator Activated Receptor Delta) ELISA Kit

products short name :

[PPAR-delta]

other names :

[peroxisome proliferator-activated receptor delta isoform 4; Peroxisome proliferator-activated receptor delta; peroxisome proliferator-activated receptor delta; PPAR-beta; PPAR-delta; nuclear hormone receptor 1; nuclear receptor subfamily 1 group C member 2; peroxisome proliferator-activated nuclear receptor beta/delta variant 2; peroxisome proliferator-activated receptor beta; peroxisome proliferator-activated receptor delta; NUCI; Nuclear hormone receptor 1; NUC1; Nuclear receptor subfamily 1 group C member 2; Peroxisome proliferator-activated receptor beta; PPAR-beta]

products gene name :

[PPAR-delta]

other gene names :

[PPARD; PPARD; FAAR; NUC1; NUCI; NR1C2; NUCII; PPARB; NR1C2; PPARB; PPAR-delta; NUC1; PPAR-beta]

uniprot entry name :

PPARD_HUMAN

reactivity :

Human

sequence length :

343

specificity :

This kit recognizes Human PPAR-gamma in samples. No significant cross-reactivity or interference between Human PPAR-gamma and analogues was observed.

storage stability :

Store at 4 degree C.

image1 heading :

Typical Testing Data/Standard Curve (for reference only)

other info1 :

Samples: Serum, Plasma And Other Biological Fluids. Assay Type: Quantitative Sandwich. Detection Range: 0.16-10ng/mL. Sensitivity: 0.1ng/mL.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human PPAR-gamma were tested 20 times on one plate, respectively. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human PPAR-gamma were tested on 3 different plates, 20 replicates in each plate.

products description :

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Human PPAR-gamma concentrations in serum, plasma and other biological fluids. Principle of the Assay: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human PPAR-gamma. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human PPAR-gamma and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human PPAR-gamma, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human PPAR-gamma. You can calculate the concentration of Human PPAR-gamma in the samples by comparing the OD of the samples to the standard curve.

ncbi gi num :

284807159

ncbi acc num :

NP_001165291.1

ncbi gb acc num :

NM_001171820.1

uniprot acc num :

Q03181

ncbi mol weight :

38,855 Da

ncbi pathways :

Acute Myeloid Leukemia Pathway (83117); Acute Myeloid Leukemia Pathway (529); Adipogenesis Pathway (198832); Energy Metabolism Pathway (198907); Fatty Acid, Triacylglycerol, And Ketone Body Metabolism Pathway (160977); Gene Expression Pathway (105937); Generic Transcription Pathway (105938); Import Of Palmitoyl-CoA Into The Mitochondrial Matrix Pathway (106108); Metabolism Pathway (477135); Metabolism Of Lipids And Lipoproteins Pathway (160976)

ncbi summary :

This gene encodes a member of the peroxisome proliferator-activated receptor (PPAR) family. PPARs are nuclear hormone receptors that bind peroxisome proliferators and control the size and number of peroxisomes produced by cells. PPARs mediate a variety of biological processes, and may be involved in the development of several chronic diseases, including diabetes, obesity, atherosclerosis, and cancer. This protein is a potent inhibitor of ligand-induced transcription activity of PPAR alpha and PPAR gamma. It may function as an integrator of transcription repression and nuclear receptor signaling. The expression of this gene is found to be elevated in colorectal cancer cells. The elevated expression can be repressed by adenomatosis polyposis coli (APC), a tumor suppressor protein related to APC/beta-catenin signaling pathway. Knockout studies in mice suggested the role of this protein in myelination of the corpus callosum, lipid metabolism, and epidermal cell proliferation. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jan 2010]

uniprot summary :

PPAR-delta: Ligand-activated transcription factor. Receptor that binds peroxisome proliferators such as hypolipidemic drugs and fatty acids. Has a preference for poly-unsaturated fatty acids, such as gamma-linoleic acid and eicosapentanoic acid. Once activated by a ligand, the receptor binds to promoter elements of target genes. Regulates the peroxisomal beta-oxidation pathway of fatty acids. Functions as transcription activator for the acyl-CoA oxidase gene. Decreases expression of NPC1L1 once activated by a ligand. Belongs to the nuclear hormone receptor family. NR1 subfamily. 3 isoforms of the human protein are produced by alternative splicing. Protein type: DNA-binding; Nuclear receptor. Chromosomal Location of Human Ortholog: 6p21.2. Cellular Component: nucleoplasm; nucleus. Molecular Function: NF-kappaB binding; ligand-dependent nuclear receptor activity; protein binding; DNA binding; zinc ion binding; sequence-specific DNA binding; transcription coactivator activity; steroid hormone receptor activity; drug binding; lipid binding; transcription factor activity. Biological Process: proteoglycan metabolic process; wound healing; negative regulation of smooth muscle cell proliferation; heart development; positive regulation of transcription, DNA-dependent; negative regulation of collagen biosynthetic process; positive regulation of epidermis development; negative regulation of smooth muscle cell migration; glucose transport; decidualization; negative regulation of transcription from RNA polymerase II promoter; positive regulation of vasodilation; vitamin A metabolic process; response to vitamin A; positive regulation of cell proliferation; response to glucose stimulus; axon ensheathment; cell differentiation; negative regulation of epithelial cell proliferation; transcription initiation from RNA polymerase II promoter; cholesterol metabolic process; generation of precursor metabolites and energy; intracellular receptor-mediated signaling pathway; glucose metabolic process; positive regulation of insulin secretion; fatty acid catabolic process; keratinocyte proliferation; keratinocyte migration; cell-substrate adhesion; phospholipid biosynthetic process; positive regulation of phosphoinositide 3-kinase cascade; regulation of transcription from RNA polymerase II promoter; cell proliferation; fatty acid beta-oxidation; negative regulation of inflammatory response; mRNA transcription; positive regulation of fat cell differentiation; steroid hormone mediated signaling; gene expression; lipid metabolic process; response to activity; negative regulation of cell growth; negative regulation of transcription, DNA-dependent; embryo implantation; fatty acid transport; negative regulation of apoptosis

size1 :

24-Strip-Wells

price1 :

215 USD

size2 :

48-Strip-Wells

price2 :

410

size3 :

96-Strip-Wells

price3 :

490

size4 :

5x96-Strip-Wells

price4 :

2040

size5 :

10x96-Strip-Wells

price5 :

3590