Human VCL (Vinculin) ELISA Kit | MyBioSource MBS2500276 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human VCL (Vinculin) ELISA Kit

catalog :

MBS2500276

quantity :

24-Strip-Wells

price :

215 USD

product information

catalog number :

MBS2500276

products type :

ELISA Kit

products full name :

Human VCL (Vinculin) ELISA Kit

products short name :

[VCL]

other names :

[vinculin isoform VCL; Vinculin; vinculin; epididymis luminal protein 114; metavinculin; vinculin; Metavinculin; MV]

products gene name :

[VCL]

other gene names :

[VCL; VCL; MV; MVCL; CMD1W; CMH15; HEL114; MV]

uniprot entry name :

VINC_HUMAN

reactivity :

Human

sequence length :

1066

specificity :

This kit recognizes natural and recombinant Human VCL. No significant cross-reactivity or interference between Human VCL and analogues was observed.

storage stability :

Store at 4 degree C.

image1 heading :

Typical Testing Data/Standard Curve (for reference only)

other info1 :

Samples: Serum, Plasma, Biological Fluids. Assay Type: Sandwich. Detection Range: 78.125-5000pg/mL. Sensitivity: Min: 46.875pg/mL; Max: 5000pg/mL

products description :

Intended Uses This ELISA kit applies to the in vitro quantitative determination of Human VCL concentrations in serum, plasma and other biological fluids. Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to VCL. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for VCL and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain VCL, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of VCL. You can calculate the concentration of VCL in the samples by comparing the OD of the samples to the standard curve.

ncbi gi num :

4507877

ncbi acc num :

NP_003364.1

ncbi gb acc num :

NM_003373.3

uniprot acc num :

P18206

ncbi mol weight :

24,904 Da

ncbi pathways :

Adherens Junction Pathway (83070); Adherens Junction Pathway (481); Amoebiasis Pathway (167324); Amoebiasis Pathway (167191); Bacterial Invasion Of Epithelial Cells Pathway (149807); Bacterial Invasion Of Epithelial Cells Pathway (148661); Focal Adhesion Pathway (198795); Focal Adhesion Pathway (83067); Focal Adhesion Pathway (478); Hemostasis Pathway (106028)

ncbi summary :

Vinculin is a cytoskeletal protein associated with cell-cell and cell-matrix junctions, where it is thought to function as one of several interacting proteins involved in anchoring F-actin to the membrane. Defects in VCL are the cause of cardiomyopathy dilated type 1W. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Multiple alternatively spliced transcript variants have been found for this gene, but the biological validity of some variants has not been determined. [provided by RefSeq, Jul 2008]

uniprot summary :

Vinculin: Actin filament (F-actin)-binding protein involved in cell-matrix adhesion and cell-cell adhesion. Regulates cell- surface E-cadherin expression and potentiates mechanosensing by the E-cadherin complex. May also play important roles in cell morphology and locomotion. Exhibits self-association properties. Interacts with NRAP and SORBS1. Interacts with TLN1. Interacts with SYNM. Interacts with CTNNB1 and this interaction is necessary for its localization to the cell-cell junctions and for its function in regulating cell surface expression of E-cadherin. Metavinculin is muscle-specific. Belongs to the vinculin/alpha-catenin family. 3 isoforms of the human protein are produced by alternative splicing. Protein type: Motility/polarity/chemotaxis; Cytoskeletal; Cell adhesion. Chromosomal Location of Human Ortholog: 10q22.2. Cellular Component: focal adhesion; costamere; protein complex; extracellular region; intercellular junction; fascia adherens; cytosol; actin cytoskeleton; cell-matrix junction; cell-cell adherens junction; adherens junction; cytoskeleton; plasma membrane; vesicle. Molecular Function: protein binding; cadherin binding; beta-catenin binding; actin binding; structural molecule activity; alpha-catenin binding. Biological Process: lamellipodium biogenesis; platelet activation; apical junction assembly; platelet degranulation; muscle contraction; axon extension; cell-matrix adhesion; morphogenesis of an epithelium; cell motility; cell adhesion; blood coagulation; negative regulation of cell migration. Disease: Cardiomyopathy, Dilated, 1w

size1 :

24-Strip-Wells

price1 :

215 USD

size2 :

48-Strip-Wells

price2 :

410

size3 :

96-Strip-Wells

price3 :

490

size4 :

5x96-Strip-Wells

price4 :

2040

size5 :

10x96-Strip-Wells

price5 :

3590