Human OC/BGP (Osteocalcin) ELISA Kit | MyBioSource MBS2500167 product information

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product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human OC/BGP (Osteocalcin) ELISA Kit

catalog :

MBS2500167

quantity :

24-Strip-Wells

price :

190 USD

product information

catalog number :

MBS2500167

products type :

ELISA Kit

products full name :

Human OC/BGP (Osteocalcin) ELISA Kit

products short name :

[OC/BGP]

other names :

[osteocalcin preproprotein; Osteocalcin; osteocalcin; bone Gla protein; bone gamma-carboxyglutamate (gla) protein (osteocalcin); gamma-carboxyglutamic acid-containing protein; bone gamma-carboxyglutamate (gla) protein; Bone Gla protein; BGP; Gamma-carboxyglutamic acid-containing protein]

products gene name :

[OC/BGP]

other gene names :

[BGLAP; BGLAP; OC; BGP; OCN; BGP]

uniprot entry name :

OSTCN_HUMAN

reactivity :

Human

sequence length :

100

specificity :

This kit recognizes Human CHGB in samples. No significant cross-reactivity or interference between HumanCHGBand analogues was observed.

storage stability :

Store at 4 degree C.

image1 heading :

Typical Testing Data/Standard Curve (for reference only)

other info1 :

Samples: Serum, Plasma And Other Biological Fluids. Assay Type: Quantitative Sandwich. Detection Range: 62.5-4000pg/mL. Sensitivity: 37.5pg/mL.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, midrange and high level HumanCHGBwere tested 20times on one plate, respectively. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, midrange and high level HumanCHGB were testedon 3 different plates, 20 replicates in each plate.

products description :

Intended Uses: This ELISA kit can be applied to the in vitro quantitative determination of Human CHGB concentrations in serum, plasma and other biological fluids. Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been precoated with an antibody specific to Human CHGB. Standards or samples are added to appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibodies specific for Human CHGB and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well successively and incubated. After incubation, free components are washed away. Then the Substrate Reagent is added to each well, only those wells that contain Human CHGB, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction will be terminated by adding Stop Solution and appears yellow in color. The optical density (OD) can be measured with spectrophotometry at a wavelength of 450 nm +/-2 nm. The OD value is proportional to the concentration of Human CHGB. The concentration of Human CHGB in samples can be calculated by comparing the OD of the samples with the standard curve.

ncbi gi num :

40316933

ncbi acc num :

NP_954642.1

ncbi gb acc num :

NM_199173.4

uniprot acc num :

P02818

ncbi mol weight :

10,963 Da

ncbi pathways :

FGF Signaling Pathway (137989); Glucocorticoid Receptor Regulatory Network Pathway (138014); Interleukin-11 Signaling Pathway (698753); Notch-mediated HES/HEY Network Pathway (169347); Regulation Of Retinoblastoma Protein Pathway (137916); Validated Transcriptional Targets Of AP1 Family Members Fra1 And Fra2 Pathway (169349)

ncbi summary :

This gene encodes a highly abundant bone protein secreted by osteoblasts that regulates bone remodeling and energy metabolism. The encoded protein contains a Gla (gamma carboxyglutamate) domain, which functions in binding to calcium and hydroxyapatite, the mineral component of bone. Serum osteocalcin levels may be negatively correlated with metabolic syndrome. Read-through transcription exists between this gene and the neighboring upstream gene, PMF1 (polyamine-modulated factor 1), but the encoded protein only shows sequence identity with the upstream gene product. [provided by RefSeq, Jun 2015]

uniprot summary :

osteocalcin: Constitutes 1-2% of the total bone protein. It binds strongly to apatite and calcium. Belongs to the osteocalcin/matrix Gla protein family. Protein type: Cell development/differentiation; Secreted, signal peptide; Secreted. Chromosomal Location of Human Ortholog: 1q22. Cellular Component: Golgi apparatus; extracellular space; rough endoplasmic reticulum; cytoplasm; dendrite; perikaryon. Molecular Function: structural constituent of bone; hydroxyapatite binding; calcium ion binding; structural molecule activity. Biological Process: response to drug; response to gravity; regulation of bone resorption; response to glucocorticoid stimulus; cell aging; response to testosterone stimulus; bone mineralization; osteoblast development; odontogenesis; osteoblast differentiation; response to ethanol; regulation of osteoclast differentiation; response to vitamin D; response to vitamin K; response to estrogen stimulus; response to zinc ion; response to mechanical stimulus; response to hydroxyisoflavone; response to activity; cell adhesion; regulation of bone mineralization; skeletal development

size1 :

24-Strip-Wells

price1 :

190 USD

size2 :

48-Strip-Wells

price2 :

310

size3 :

96-Strip-Wells

price3 :

355

size4 :

5x96-Strip-Wells

price4 :

1435

size5 :

10x96-Strip-Wells

price5 :

2805