Canine HP (Haptoglobulin) ELISA Kit | MyBioSource MBS2500069 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Canine HP (Haptoglobulin) ELISA Kit

catalog :

MBS2500069

quantity :

24-Strip-Wells

price :

255 USD

more info or order :

MyBioSource product webpage

image

image 1 :

product information

catalog number :

MBS2500069

products type :

ELISA Kit

products full name :

Canine HP (Haptoglobulin) ELISA Kit

products short name :

[HP]

other names :

[haptoglobin isoform 2 preproprotein; Haptoglobin; haptoglobin; binding peptide; haptoglobin alpha(1S)-beta; haptoglobin alpha(2FS)-beta; haptoglobin, alpha polypeptide; haptoglobin, beta polypeptide; zonulin; haptoglobin; ZonulinCleaved into the following 2 chains:Haptoglobin alpha chain; Haptoglobin beta chain]

products gene name :

[HP]

other gene names :

[HP; HP; BP; HPA1S; HP2ALPHA2]

uniprot entry name :

HPT_HUMAN

reactivity :

Canine

sequence length :

347

specificity :

This kit recognizes Canine HP in samples. No Significant cross -reactivity or interference between Canine HP and analogues was observed.

storage stability :

Store at 4 degree C.

image1 heading :

Typical Testing Data/Standard Curve (for reference only)

other info1 :

Samples: Serum, Plasma, Biological Fluids. Assay Type: Sandwich. Detection Range: 1.563-100ng/mL. Sensitivity: Min: 0.938ng/mL; Max: 100ng/mL

products description :

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of CanineHP concentrations in serum, plasma and other biological fluids. Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Canine HP. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Canine HP and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Canine HP, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Canine HP. You can calculate the concentration of Canine HP in the samples by comparing the OD of the samples to the standard curve.

ncbi gi num :

186910296

ncbi acc num :

NP_001119574.1

ncbi gb acc num :

NM_001126102.1

uniprot acc num :

P00738

ncbi mol weight :

38,452 Da

ncbi pathways :

Binding And Uptake Of Ligands By Scavenger Receptors Pathway (771599); Scavenging Of Heme From Plasma Pathway (771600); Amb2 Integrin Signaling Pathway (137945)

ncbi summary :

This gene encodes a preproprotein, which is processed to yield both alpha and beta chains, which subsequently combine as a tetramer to produce haptoglobin. Haptoglobin functions to bind free plasma hemoglobin, which allows degradative enzymes to gain access to the hemoglobin, while at the same time preventing loss of iron through the kidneys and protecting the kidneys from damage by hemoglobin. Mutations in this gene and/or its regulatory regions cause ahaptoglobinemia or hypohaptoglobinemia. This gene has also been linked to diabetic nephropathy, the incidence of coronary artery disease in type 1 diabetes, Crohn's disease, inflammatory disease behavior, primary sclerosing cholangitis, susceptibility to idiopathic Parkinson's disease, and a reduced incidence of Plasmodium falciparum malaria. The protein encoded also exhibits antimicrobial activity against bacteria. A similar duplicated gene is located next to this gene on chromosome 16. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2014]

uniprot summary :

HP: Haptoglobin combines with free plasma hemoglobin, preventing loss of iron through the kidneys and protecting the kidneys from damage by hemoglobin, while making the hemoglobin accessible to degradative enzymes. Defects in HP are the cause of anhaptoglobinemia (AHP). AHP is a condition characterized by the absence of the serum glycoprotein haptoglobin. Serum levels of haptoglobin vary among normal persons: levels are low in the neonatal period and in the elderly, differ by population, and can be influenced by environmental factors, such as infection. Secondary hypohaptoglobinemia can occur as a consequence of hemolysis, during which haptoglobin binds to free hemoglobin. Belongs to the peptidase S1 family. Protein type: Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 16q22.2. Cellular Component: extracellular space; extracellular region. Molecular Function: antioxidant activity; protein binding; hemoglobin binding; catalytic activity. Biological Process: receptor-mediated endocytosis; response to hydrogen peroxide; metabolic process; immune system process; negative regulation of oxidoreductase activity; defense response to bacterium; acute-phase response; defense response. Disease: Anhaptoglobinemia

size1 :

24-Strip-Wells

price1 :

255 USD

size2 :

48-Strip-Wells

price2 :

490

size3 :

96-Strip-Wells

price3 :

585

size4 :

5x96-Strip-Wells

price4 :

2475

size5 :

10x96-Strip-Wells

price5 :

4360

more info or order :

MyBioSource product webpage