Human MYBPC3 (Myosin Binding Protein C, Cardiac) ELISA Kit | MyBioSource MBS2500055 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human MYBPC3 (Myosin Binding Protein C, Cardiac) ELISA Kit

catalog :

MBS2500055

quantity :

24-Strip-Wells

price :

215 USD

product information

catalog number :

MBS2500055

products type :

ELISA Kit

products full name :

Human MYBPC3 (Myosin Binding Protein C, Cardiac) ELISA Kit

products short name :

[MYBPC3]

other names :

[myosin-binding protein C, cardiac-type; Myosin-binding protein C, cardiac-type; myosin-binding protein C, cardiac-type; C-protein, cardiac muscle isoform; myosin binding protein C, cardiac; C-protein, cardiac muscle isoform]

products gene name :

[MYBPC3]

other gene names :

[MYBPC3; MYBPC3; FHC; CMH4; CMD1MM; LVNC10; MYBP-C; Cardiac MyBP-C]

uniprot entry name :

MYPC3_HUMAN

reactivity :

Human

sequence length :

1274

specificity :

This kit recognizes natural and recombinant Human MYBPC3. No significant cross-reactivity or interference between Human MYBPC3 and analogues was observed.

storage stability :

Store at 4 degree C.

image1 heading :

Typical Testing Data/Standard Curve (for reference only)

other info1 :

Samples: Serum, Plasma, Biological Fluids. Assay Type: Sandwich. Detection Range: 0.156-10ng/mL. Sensitivity: Min: 0.094ng/mL; Max: 10ng/mL

other info2 :

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Human MYBPC3 concentrations in serum, plasma and other biological fluids.

products description :

Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to MYBPC3. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for MYBPC3 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain MYBPC3, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of MYBPC3. You can calculate the concentration of MYBPC3 in the samples by comparing the OD of the samples to the standard curve.

ncbi gi num :

148596957

ncbi acc num :

NP_000247.2

ncbi gb acc num :

NM_000256.3

uniprot acc num :

Q14896

ncbi mol weight :

140,703 Da

ncbi pathways :

Dilated Cardiomyopathy Pathway (121494); Dilated Cardiomyopathy Pathway (121285); Hypertrophic Cardiomyopathy (HCM) Pathway (114229); Hypertrophic Cardiomyopathy (HCM) Pathway (106591); Muscle Contraction Pathway (106261); Striated Muscle Contraction Pathway (198903); Striated Muscle Contraction Pathway (106262)

ncbi summary :

MYBPC3 encodes the cardiac isoform of myosin-binding protein C. Myosin-binding protein C is a myosin-associated protein found in the cross-bridge-bearing zone (C region) of A bands in striated muscle. MYBPC3, the cardiac isoform, is expressed exclussively in heart muscle. Regulatory phosphorylation of the cardiac isoform in vivo by cAMP-dependent protein kinase (PKA) upon adrenergic stimulation may be linked to modulation of cardiac contraction. Mutations in MYBPC3 are one cause of familial hypertrophic cardiomyopathy. [provided by RefSeq, Jul 2008]

uniprot summary :

MYBPC3: Thick filament-associated protein located in the crossbridge region of vertebrate striated muscle a bands. In vitro it binds MHC, F-actin and native thin filaments, and modifies the activity of actin-activated myosin ATPase. It may modulate muscle contraction or may play a more structural role. Belongs to the immunoglobulin superfamily. MyBP family. Protein type: Myosin-binding. Chromosomal Location of Human Ortholog: 11p11.2. Cellular Component: sarcomere; striated muscle thick filament; cytosol; A band. Molecular Function: identical protein binding; ATPase activator activity; myosin binding; structural constituent of muscle; metal ion binding; titin binding; actin binding; myosin heavy chain binding. Biological Process: heart morphogenesis; positive regulation of ATPase activity; regulation of heart rate; regulation of muscle filament sliding; regulation of striated muscle contraction; ventricular cardiac muscle morphogenesis; sarcomere organization; cell adhesion; myosin filament assembly; cardiac muscle contraction; muscle filament sliding. Disease: Left Ventricular Noncompaction 10; Cardiomyopathy, Familial Hypertrophic, 4

size1 :

24-Strip-Wells

price1 :

215 USD

size2 :

48-Strip-Wells

price2 :

410

size3 :

96-Strip-Wells

price3 :

490

size4 :

5x96-Strip-Wells

price4 :

2040

size5 :

10x96-Strip-Wells

price5 :

3590