Human AIF1 (Allograft Inflammatory Factor 1) ELISA Kit | MyBioSource MBS2500028 product information

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product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human AIF1 (Allograft Inflammatory Factor 1) ELISA Kit

catalog :

MBS2500028

quantity :

24-Strip-Wells

price :

215 USD

product information

catalog number :

MBS2500028

products type :

ELISA Kit

products full name :

Human AIF1 (Allograft Inflammatory Factor 1) ELISA Kit

products short name :

[AIF1]

other names :

[AIF1, partial; Allograft inflammatory factor 1; allograft inflammatory factor 1; allograft inflammatory factor-1 splice variant Hara-1; interferon gamma responsive transcript; ionized calcium-binding adapter molecule 1; protein G1; allograft inflammatory factor 1; Ionized calcium-binding adapter molecule 1; Protein G1]

products gene name :

[AIF1]

other gene names :

[AIF1; AIF1; IBA1; IRT1; AIF-1; IRT-1; G1; IBA1; AIF-1]

uniprot entry name :

AIF1_HUMAN

reactivity :

Human

sequence length :

specificity :

This kit recognizes natural and recombinant Human AIF1. No significant cross-reactivity or interference between Human AIF1 and analogues was observed.

storage stability :

Store at 4 degree C.

image1 heading :

Typical Testing Data/Standard Curve (for reference only)

other info1 :

Samples: Serum, Plasma, Biological Fluids. Assay Type: Sandwich. Detection Range: 31.25-2000pg/mL. Sensitivity: Min: 18.75pg/mL; Max: 2000pg/mL

other info2 :

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Human AIF1 concentrations in serum, plasma and other biological fluids.

products description :

Principle of the assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to AIF1. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for AIF1 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain AIF1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of AIF1. You can calculate the concentration of AIF1 in the samples by comparing the OD of the samples to the standard curve.

ncbi gi num :

49457302

ncbi acc num :

CAG46950.1

uniprot acc num :

Q9UIV4

ncbi mol weight :

14,617 Da

ncbi pathways :

Spinal Cord Injury Pathway (739007)

ncbi summary :

This gene is induced by cytokines and interferon. Its protein product is thought to be involved in negative regulation of growth of vascular smooth muscle cells, which contributes to the anti-inflammatory response to vessel wall trauma. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2008]

uniprot summary :

AIF1: Actin-binding protein that enhances membrane ruffling and RAC activation. Enhances the actin-bundling activity of LCP1. Binds calcium. Plays a role in RAC signaling and in phagocytosis. May play a role in macrophage activation and function. Promotes the proliferation of vascular smooth muscle cells and of T- lymphocytes. Enhances lymphocyte migration. Plays a role in vascular inflammation. 3 isoforms of the human protein are produced by alternative splicing. Protein type: Cytoskeletal. Chromosomal Location of Human Ortholog: 6p21.3. Cellular Component: lamellipodium; perinuclear region of cytoplasm; cytoplasm; actin filament; perikaryon; cytosol; nucleus; phagocytic cup. Molecular Function: actin filament binding; calcium ion binding. Biological Process: actin filament bundle formation; positive regulation of nitric oxide biosynthetic process; negative regulation of smooth muscle cell proliferation; positive regulation of smooth muscle cell proliferation; microglial cell activation; Rac protein signal transduction; cellular response to hormone stimulus; actin filament polymerization; positive regulation of muscle hyperplasia; cellular response to extracellular stimulus; positive regulation of T cell proliferation; response to axon injury; positive regulation of protein amino acid phosphorylation; inflammatory response; phagocytosis, engulfment; response to electrical stimulus; negative regulation of apoptosis

size1 :

24-Strip-Wells

price1 :

215 USD

size2 :

48-Strip-Wells

price2 :

410

size3 :

96-Strip-Wells

price3 :

490

size4 :

5x96-Strip-Wells

price4 :

2040

size5 :

10x96-Strip-Wells

price5 :

3590