Human PGAM1 (Phosphoglycerate Mutase 1) ELISA Kit | MyBioSource MBS2500005 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human PGAM1 (Phosphoglycerate Mutase 1) ELISA Kit

catalog :

MBS2500005

quantity :

24-Strip-Wells

price :

215 USD

product information

catalog number :

MBS2500005

products type :

ELISA Kit

products full name :

Human PGAM1 (Phosphoglycerate Mutase 1) ELISA Kit

products short name :

[PGAM1]

other names :

[phosphoglycerate mutase 1; Phosphoglycerate mutase 1; phosphoglycerate mutase 1; BPG-dependent PGAM 1; epididymis secretory protein Li 35; phosphoglycerate mutase A, nonmuscle form; phosphoglycerate mutase isozyme B; phosphoglycerate mutase 1 (brain); BPG-dependent PGAM 1; Phosphoglycerate mutase isozyme B; PGAM-B]

products gene name :

[PGAM1]

other gene names :

[PGAM1; PGAM1; PGAMA; PGAM-B; HEL-S-35; PGAMA; PGAM-B]

uniprot entry name :

PGAM1_HUMAN

reactivity :

Human

sequence length :

254

specificity :

This kit recognizes natural and some recombinant Human PGAM1. No significant crossreactivity or interference between Human PGAM1 and analogues was observed.

storage stability :

Store at 4 degree C.

image1 heading :

Typical Testing Data/Standard Curve (for reference only)

other info1 :

Samples: Serum, Plasma, Biological Fluids. Assay Type: Sandwich. Detection Range: 0.781-50ng/mL. Sensitivity: Min: 0.469ng/mL; Max: 50ng/mL

products description :

Intended Uses: This ELISA kit can be applied to the in vitro quantitative determination of Human PGAM1 concentrations in serum, plasma and other biological fluids. Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been precoated with an antibody specific to Human PGAM1. Standards or samples are added to appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibodies specific for Human PGAM1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well successively and incubated. After incubation, free components are washed away. Then the Substrate Reagent is added to each well, only those wells that contain Human PGAM1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction will be terminated by adding Stop Solution and appears yellow in color. The optical density (OD) can be measured with spectrophotometry at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human PGAM1. The concentration of Human PGAM1 in samples can be calculated by comparing the OD of the samples with the standard curve.

ncbi gi num :

4505753

ncbi acc num :

NP_002620.1

ncbi gb acc num :

NM_002629.2

uniprot acc num :

P18669

ncbi mol weight :

28,804 Da

ncbi pathways :

Biosynthesis Of Amino Acids Pathway (790012); Biosynthesis Of Amino Acids Pathway (795174); Carbon Metabolism Pathway (814926); Carbon Metabolism Pathway (817567); Central Carbon Metabolism In Cancer Pathway (1059538); Central Carbon Metabolism In Cancer Pathway (1084231); Disease Pathway (530764); Gluconeogenesis Pathway (106204); Gluconeogenesis, Oxaloacetate = Fructose-6P Pathway (413342); Gluconeogenesis, Oxaloacetate = Fructose-6P Pathway (468196)

ncbi summary :

Phosphoglyceric acid mutase (EC 2.7.5.3) is widely distributed in mammalian tissues where it catalyzes the reversible reaction of 3-phosphoglycerate (3-PGA) to 2-phosphoglycerate (2-PGA) in the glycolytic pathway (summary by Chen et al., 1974 [PubMed 4811757]).[supplied by OMIM, Nov 2010]

uniprot summary :

PGAM1: Interconversion of 3- and 2-phosphoglycerate with 2,3- bisphosphoglycerate as the primer of the reaction. Can also catalyze the reaction of EC 5.4.2.4 (synthase) and EC 3.1.3.13 (phosphatase), but with a reduced activity. Homodimer. In mammalian tissues there are two types of phosphoglycerate mutase isozymes: type-M in muscles and type-B in other tissues. Belongs to the phosphoglycerate mutase family. BPG- dependent PGAM subfamily. Protein type: Phosphatase (non-protein); Isomerase; EC 3.1.3.13; EC 5.4.2.11; Carbohydrate Metabolism - glycolysis and gluconeogenesis; EC 5.4.2.4. Chromosomal Location of Human Ortholog: 10q25.3. Cellular Component: membrane; cytoplasm; cytosol. Molecular Function: protein binding; phosphoglycerate mutase activity; bisphosphoglycerate mutase activity; bisphosphoglycerate phosphatase activity; protein kinase binding. Biological Process: respiratory burst; glycolysis; dephosphorylation; regulation of glycolysis; carbohydrate metabolic process; glucose metabolic process; pathogenesis; regulation of pentose-phosphate shunt; gluconeogenesis

size1 :

24-Strip-Wells

price1 :

215 USD

size2 :

48-Strip-Wells

price2 :

410

size3 :

96-Strip-Wells

price3 :

490

size4 :

5x96-Strip-Wells

price4 :

2040

size5 :

10x96-Strip-Wells

price5 :

3590