Anti-RAB5A / RAB5 Antibody (C-Terminus) IHC-plus | MyBioSource MBS249901 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Anti-RAB5A / RAB5 Antibody (C-Terminus) IHC-plus

catalog :

MBS249901

quantity :

0.3 mg

price :

530 USD

clonality :

polyclonal

host :

goat

conjugate :

nonconjugated

reactivity :

human, mouse, rat, dog

application :

western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

more info or order :

MyBioSource product webpage

image

image 1 :

Human Testis: Formalin-Fixed, Paraffin-Embedded (FFPE)

image 2 :

Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE)

image 3 :

Immunofluorescence - anti-Rab5 antibody using B6-RPE07 cells at 1:50 dilution. Cells were fixed with 4% of PFA.

product information

catalog number :

MBS249901

products type :

Antibody

products full name :

Anti-RAB5A / RAB5 Antibody (C-Terminus) IHC-plus

products short name :

[RAB5A / RAB5]

products name syn :

[Goat Polyclonal (IgG) to Mouse RAB5A / RAB5; Mouse RAB5A / RAB5; RAB5A; Ras-related protein Rab-5A; RAS-associated protein RAB5A; RAB5]

other names :

[ras-related protein Rab-5A isoform 1; Ras-related protein Rab-5A; ras-related protein Rab-5A; RAB5A, member RAS oncogene family]

products gene name :

[RAB5A / RAB5]

other gene names :

[RAB5A; RAB5A; RAB5; RAB5]

uniprot entry name :

RAB5A_HUMAN

clonality :

Polyclonal

isotype :

IgG

host :

goat

reactivity :

Mouse, Dog, Rat, Human, Monkey

sequence length :

215

specificity :

Detects total Rab5 protein in the following human, rat and mouse whole cell lysates and transfected cells with GFP-Rab5a, GFP-Rab5b and GFP-Rab5c cds by Western blot.

purity :

Immunoaffinity purified

form :

PBS, 20% glycerol, 0.05% sodium azide.

concentration :

3 mg/mL

storage stability :

Store at 4°short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

tested application :

Immunohistochemistry (IHC) Paraffin, Immunofluorescence (IF), Western Blot (WB)

app notes :

IF (1:50 - 1:250). IHC-P (5 ug/ml). WB (1:250 - 1:2000). Optimal dilution to be determined by the researcher

image1 heading :

Immunohistochemistry - Paraffin (IHC)

image2 heading :

Immunohistochemistry - Paraffin (IHC)

image3 heading :

Immunofluorescence (IF)

image4 heading :

Western Blot (WB)

image4 description :

Western blot. Anti-Rab5 antibody at 1:1000 dilution. Tissue lysate at 100 ug per lane. Rabbit polyclonal to goat IgG (HRP) at 1:10000 dilution.

other info1 :

Immunogen: RAB5A / RAB5 antibody was raised against purified recombinant peptide derived from within residues 115 aa to the C-terminus of mouse Rab5a, Rab5b and rab5c produced in E Coli. Immunogen Species: RAB5A/RAB5 antibody was raised against Mouse.

other info2 :

Epitope: C-Terminus. Target Species: Mouse. Family/Subfamily: Ras GTPase superfamily IPR0011806/ RAS oncogene

products description :

RAB5A Antibody, Ras-related protein Rab-5A Antibody, RAS-associated protein RAB5A Antibody, RAB5 Antibody Description: The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. RAB5A is required for the fusion of plasma membranes and early endosomes.

ncbi gi num :

19923262

ncbi acc num :

NP_004153.2

ncbi gb acc num :

NM_004162.4

uniprot acc num :

P20339

ncbi pathways :

Amoebiasis Pathway (167324); Amoebiasis Pathway (167191); Amyotrophic Lateral Sclerosis (ALS) Pathway (920975); Amyotrophic Lateral Sclerosis (ALS) Pathway (83099); Amyotrophic Lateral Sclerosis (ALS) Pathway (511); EGFR1 Signaling Pathway (198782); Endocytosis Pathway (102279); Endocytosis Pathway (102181); ErbB1 Downstream Signaling Pathway (138057); Factors Involved In Megakaryocyte Development And Platelet Production Pathway (1269377)

uniprot summary :

RAB5A: Required for the fusion of plasma membranes and early endosomes. Contributes to the regulation of filopodia extension. Binds EEA1. Interacts with RIN1 and GAPVD1, which regulate its pathway, probably by acting as a GEF. Interacts with ALS2CL, RABEP1, SUN2, ZFYVE20 and RUFY1. Interacts with SGSM1 and SGSM3. Interacts with PIK3CB. Regulated by guanine nucleotide exchange factors (GEFs) which promote the exchange of bound GDP for free GTP. Belongs to the small GTPase superfamily. Rab family. Protein type: G protein, monomeric; G protein, monomeric, Rab; Motility/polarity/chemotaxis; G protein. Chromosomal Location of Human Ortholog: 3p24-p22. Cellular Component: actin cytoskeleton; axon; cell soma; cytoplasm; cytosol; dendrite; early endosome; endocytic vesicle; endosome; endosome membrane; lipid raft; melanosome; nerve terminal; phagocytic vesicle; plasma membrane; ruffle; synaptic vesicle; terminal button. Molecular Function: GDP binding; GTP binding; GTPase activity; protein binding. Biological Process: blood coagulation; early endosome to late endosome transport; endocytosis; positive regulation of exocytosis; protein transport; regulation of endocytosis; regulation of endosome size; regulation of filopodium formation; small GTPase mediated signal transduction

size1 :

0.3 mg

price1 :

530 USD

more info or order :

MyBioSource product webpage