Anti-PTGS2 / COX2 / COX-2 Antibody (C-Terminus) IHC-plus | MyBioSource MBS249808 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Anti-PTGS2 / COX2 / COX-2 Antibody (C-Terminus) IHC-plus

catalog :

MBS249808

quantity :

0.05 mL

price :

530 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat, cow

application :

western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

more info or order :

MyBioSource product webpage

image

image 1 :

Human Colon: Formalin-Fixed, Paraffin-Embedded (FFPE)

product information

catalog number :

MBS249808

products type :

Antibody

products full name :

Anti-PTGS2 / COX2 / COX-2 Antibody (C-Terminus) IHC-plus

products short name :

[PTGS2 / COX2 / COX-2]

products name syn :

[Rabbit Polyclonal to Human PTGS2 / COX2 / COX-2; Human PTGS2 / COX2 / COX-2; Cyclooxygenase-2; Cyclooxygenase 2b; HCox-2; GRIPGHS; PGH synthase 2; PGHS-2; PHS II; PHS-2; Prostaglandin H2 synthase 2; PGG/HS; Prostaglandin G/H synthase 2]

other names :

[prostaglandin G/H synthase 2; Prostaglandin G/H synthase 2; prostaglandin G/H synthase 2; prostaglandin-endoperoxide synthase 2; Cyclooxygenase-2; COX-2; PHS II; Prostaglandin H2 synthase 2; PGH synthase 2; PGHS-2; Prostaglandin-endoperoxide synthase 2]

products gene name :

[PTGS2 / COX2 / COX-2]

other gene names :

[PTGS2; PTGS2; COX2; COX-2; PHS-2; PGG/HS; PGHS-2; hCox-2; GRIPGHS; COX2; COX-2; PGH synthase 2; PGHS-2]

uniprot entry name :

PGH2_HUMAN

clonality :

Polyclonal

host :

rabbit

reactivity :

Human, Mouse, Rat, Bovine, Pig, Sheep

sequence length :

604

specificity :

Recognizes endogenous levels of PGHS-2 protein.

purity :

Immunoaffinity purified

form :

PBS, pH 7.3, 0.01% sodium azide, 30% glycerol.

concentration :

1 mg/ml

storage stability :

Store at -20°C. Aliquot to avoid freeze/thaw cycles.

tested application :

Immunohistochemistry (IHC) Paraffin, Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blot (WB)

app notes :

IHC-Paraffin (1:100). ICC(1:100-1:500). Immunofluorescence. Western Blot (1:500-1:1000). Optimal dilution to be determined by the researcher.

image1 heading :

Immunohistochemistry - Paraffin (IHC)

other info1 :

Immunogen species: PTGS2/COX2/COX-2 antibody was raised against Human. Immunogen : PTGS2 / COX2 / COX-2 antibody was raised against kLH-conjugated synthetic peptide encompassing a sequence within the C-terminal region of human PGHS-2. Antigen Type: Synthetic peptide. Note: During shipment, small volumes of product will ocassionally become entrapped in the seal of the product vial. We recommend briefly centrifuging the vial to dislodge any liquid in the container's cap prior to opening. Warning: This reagent may contain sodium azide. The chemical, physical and toxicological properties of this material have not been throughly investigated. Standard laboratory Practices should be followed. Avoid sking and eye contact, inhalation, and ingestion. Sodium azide forms hydrazoic acid under acidic conditions and may react with lead or copper plumbing to form highly explosive metal azides. On disposal, flush with large volumes of water to prevent acumulation.

other info2 :

Epitope: C-Terminus. Disclaimer: Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application. Recommended Immunohistochemistry Protocol: The following protocol is a recommendation only, and MyBioSource, Inc. makes no guarantee of the results:. Tissue Preparation: . Formalin fixation and embedding in paraffin wax. Tissue Sectioning: . Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C. Deparaffinization: . Wash dry slides in 3 changes of xylene - 5 minutes each @ RT. Rehydration: . Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT. Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT. Wash slides in 1 change of 80% alcohol - 3 minutes @ RT. Rinse slides in gentle running distilled water - 5 minutes @ RT. Antigen retrieval: . Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes. Remove from heat and let stand at room temperature in buffer - 20 minutes. Rinse in 1X TBS with Tween (TBST) -1 minute @ RT. Immunostaining: . (Do not allow tissues to dry at any time during the staining procedure) . Apply a universal protein block - 20 minutes @ RT. Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT. Rinse slides in 1 X TBST - 1 minute @ RT. Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT. Rinse slides in 1X TBST -1 minute @ RT. Apply alkaline phosphatase streptavidin - 30 minutes @ RT. Rinse slides in 1X TBST -1 minute @ RT. Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT. Wash slides in distilled water - 1 minute @ RT. Dehydrate: . (This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB) . Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT. Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT. Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT. Wash slides in 3 changes of xylene - 1 minute each @ RT. Apply coverslip

products description :

PTGS2 Antibody, COX-2 Antibody, Cyclooxygenase-2 Antibody, COX2 Antibody, Cyclooxygenase 2b Antibody, HCox-2 Antibody, GRIPGHS Antibody, PGH synthase 2 Antibody, PGHS-2 Antibody, PHS II Antibody, PHS-2 Antibody, Prostaglandin H2 synthase 2 Antibody, PGG/HS Antibody, Prostaglandin G/H synthase 2 Antibody Description: Converts arachidonate to prostaglandin H2 (PGH2), a committed step in prostanoid synthesis. Constitutively expressed in some tissues in physiological conditions, such as the endothelium, kidney and brain, and in pathological conditions, such as in cancer. PTGS2 is responsible for production of inflammatory prostaglandins. Up-regulation of PTGS2 is also associated with increased cell adhesion, phenotypic changes, resistance to apoptosis and tumor angiogenesis.

ncbi gi num :

4506265

ncbi acc num :

NP_000954.1

ncbi gb acc num :

NM_000963.3

uniprot acc num :

P35354

ncbi pathways :

Arachidonic Acid Metabolism Pathway (82991); Arachidonic Acid Metabolism Pathway (1270087); Arachidonic Acid Metabolism Pathway (366); C-MYB Transcription Factor Network Pathway (138073); C20 Prostanoid Biosynthesis Pathway (545299); C20 Prostanoid Biosynthesis Pathway (908107); Calcineurin-regulated NFAT-dependent Transcription In Lymphocytes Pathway (137993); Calcium Signaling In The CD4+ TCR Pathway (137941); Chemical Carcinogenesis Pathway (673221); Chemical Carcinogenesis Pathway (673237)

ncbi summary :

Prostaglandin-endoperoxide synthase (PTGS), also known as cyclooxygenase, is the key enzyme in prostaglandin biosynthesis, and acts both as a dioxygenase and as a peroxidase. There are two isozymes of PTGS: a constitutive PTGS1 and an inducible PTGS2, which differ in their regulation of expression and tissue distribution. This gene encodes the inducible isozyme. It is regulated by specific stimulatory events, suggesting that it is responsible for the prostanoid biosynthesis involved in inflammation and mitogenesis. [provided by RefSeq, Feb 2009]

uniprot summary :

COX-2: Mediates the formation of prostaglandins from arachidonate. May have a role as a major mediator of inflammation and/or a role for prostanoid signaling in activity-dependent plasticity. Homodimer. Belongs to the prostaglandin G/H synthase family. Protein type: Oxidoreductase; EC 1.14.99.1; Lipid Metabolism - arachidonic acid. Chromosomal Location of Human Ortholog: 1q25.2-q25.3. Cellular Component: caveola; cytoplasm; endoplasmic reticulum; endoplasmic reticulum lumen; endoplasmic reticulum membrane; neuron projection; nucleus; protein complex. Molecular Function: arachidonate 15-lipoxygenase activity; enzyme binding; heme binding; lipid binding; metal ion binding; peroxidase activity; prostaglandin-endoperoxide synthase activity; protein binding; protein homodimerization activity. Biological Process: angiogenesis; arachidonic acid metabolic process; bone mineralization; brown fat cell differentiation; cell motility; cyclooxygenase pathway; decidualization; embryo implantation; hair cycle; inflammatory response; learning; lipoxygenase pathway; memory; NAD metabolic process; negative regulation of calcium ion transport; negative regulation of cell cycle; negative regulation of cell proliferation; negative regulation of smooth muscle contraction; negative regulation of synaptic transmission, dopaminergic; nicotinamide metabolic process; ovulation; positive regulation of apoptosis; positive regulation of fever; positive regulation of NF-kappaB import into nucleus; positive regulation of nitric oxide biosynthetic process; positive regulation of prostaglandin biosynthetic process; positive regulation of smooth muscle cell proliferation; positive regulation of smooth muscle contraction; positive regulation of synaptic plasticity; positive regulation of synaptic transmission, glutamatergic; positive regulation of vasoconstriction; prostaglandin biosynthetic process; prostaglandin metabolic process; regulation of blood pressure; regulation of inflammatory response; response to drug; response to estradiol stimulus; response to fructose stimulus; response to glucocorticoid stimulus; response to lipopolysaccharide; response to lithium ion; response to manganese ion; response to oxidative stress; response to vitamin D; sensory perception of pain; vitamin metabolic process; water-soluble vitamin metabolic process

size1 :

0.05 mL

price1 :

530 USD

more info or order :

MyBioSource product webpage