Anti-LYVE1 Antibody (aa46-77) IHC-plus | MyBioSource MBS249796 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Anti-LYVE1 Antibody (aa46-77) IHC-plus

catalog :

MBS249796

quantity :

0.2 mL

price :

530 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse

application :

western blot, immunohistochemistry, immunohistochemistry - paraffin section

more info or order :

MyBioSource product webpage

image

image 1 :

Anti-LYVE1 / LYVE-1 antibody IHC staining of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.

image 2 :

Western blot of XLKD1 (arrow) using rabbit polyclonal XLKD1 Antibody (C61). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the XLKD1 gene.

image 3 :

XLKD1 Antibody western blot of Y79 cell line lysates (35 ug/lane). The XLKD1 antibody detected the XLKD1 protein (arrow).

product information

catalog number :

MBS249796

products type :

Antibody

products full name :

Anti-LYVE1 Antibody (aa46-77) IHC-plus

products short name :

[LYVE1]

products name syn :

[Rabbit Polyclonal to Human LYVE1; Human LYVE1; CRSBP-1; CRSBP1; Hyaluronic acid receptor; LYVE-1; XLKD1; HAR]

other names :

[lymphatic vessel endothelial hyaluronic acid receptor 1; Lymphatic vessel endothelial hyaluronic acid receptor 1; lymphatic vessel endothelial hyaluronic acid receptor 1; lymphatic vessel endothelial hyaluronan receptor 1; Cell surface retention sequence-binding protein 1; CRSBP-1; Extracellular link domain-containing protein 1; Hyaluronic acid receptor]

products gene name :

[LYVE1]

other gene names :

[LYVE1; LYVE1; HAR; XLKD1; LYVE-1; CRSBP-1; CRSBP1; HAR; XLKD1; LYVE-1; CRSBP-1]

uniprot entry name :

LYVE1_HUMAN

clonality :

Polyclonal

host :

Rabbit

reactivity :

Mouse, Human

sequence positions :

[46-77]

specificity :

This XLKD1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 46-77 amino acids from the N-terminal region of human XLKD1.

purity :

Immunoaffinity purified

form :

PBS, 0.09% sodium azide

storage stability :

Maintain refrigerated at 2°C to 8°C for up to 6 months. For long term storage store at -20°C.

tested application :

Immunohistochemistry (IHC) Paraffin, Western Blot (WB)

app notes :

IHC-P (1:100). WB (1:1000). Optimal dilution to be determined by the researcher.

image1 heading :

Immunohistochemistry (IHC)

image2 heading :

Western Blot (WB)

image3 heading :

Western Blot (WB)

image4 heading :

Western Blot (WB)

image4 description :

The anti-XLKD1 N-term antibody is used in Western blot to detect XLKD1 in mouse liver tissue lysate.

other info1 :

Immunogen: LYVE1 antibody was raised against kLH conjugated synthetic peptide selected from the N-terminal region of human XLKD1. Immunogen Description: KLH conjugated synthetic peptide selected from the N-terminal region of human XLKD1. Immunogen Type: Synthetic peptide

other info2 :

Antigen Modification: aa46-77. Target Species: Human. Usage: This antibody does not work for IHC with samples fixed in 4% PFA. Disclaimer: Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application. Recommended Immunohistochemistry Protocol: The following protocol is a recommendation only, and MyBioSource, Inc. makes no guarantee of the results:. Tissue Preparation: . Formalin fixation and embedding in paraffin wax. Tissue Sectioning: . Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C. Deparaffinization: . Wash dry slides in 3 changes of xylene - 5 minutes each @ RT. Rehydration: . Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT. Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT. Wash slides in 1 change of 80% alcohol - 3 minutes @ RT. Rinse slides in gentle running distilled water - 5 minutes @ RT. Antigen retrieval: . Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes. Remove from heat and let stand at room temperature in buffer - 20 minutes. Rinse in 1X TBS with Tween (TBST) -1 minute @ RT. Immunostaining: . (Do not allow tissues to dry at any time during the staining procedure) . Apply a universal protein block - 20 minutes @ RT. Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT. Rinse slides in 1 X TBST - 1 minute @ RT. Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT. Rinse slides in 1X TBST -1 minute @ RT. Apply alkaline phosphatase streptavidin - 30 minutes @ RT. Rinse slides in 1X TBST -1 minute @ RT. Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT. Wash slides in distilled water - 1 minute @ RT. Dehydrate: . (This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB) . Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT. Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT. Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT. Wash slides in 3 changes of xylene - 1 minute each @ RT. Apply coverslip

products description :

LYVE1 Antibody, CRSBP-1 Antibody, CRSBP1 Antibody, Hyaluronic acid receptor Antibody, LYVE-1 Antibody, XLKD1 Antibody, HAR Antibody Description: Ligand-specific transporter trafficking between intracellular organelles (TGN) and the plasma membrane. Plays a role in autocrine regulation of cell growth mediated by growth regulators containing cell surface retention sequence binding (CRS). May act as a hyaluronan (HA) transporter, either mediating its uptake for catabolism within lymphatic endothelial cells themselves, or its transport into the lumen of afferent lymphatic vessels for subsequent re-uptake and degradation in lymph nodes.

ncbi gi num :

40549451

ncbi acc num :

NP_006682.2

ncbi gb acc num :

NM_006691.3

ncbi mol weight :

35,213 Da

ncbi pathways :

Glycosaminoglycan Metabolism Pathway (1269972); Hyaluronan Metabolism Pathway (1269973); Hyaluronan Uptake And Degradation Pathway (1269975); Metabolism Pathway (1269956); Metabolism Of Carbohydrates Pathway (1269957)

ncbi summary :

This gene encodes a type I integral membrane glycoprotein. The encoded protein acts as a receptor and binds to both soluble and immobilized hyaluronan. This protein may function in lymphatic hyaluronan transport and have a role in tumor metastasis. [provided by RefSeq, Jul 2008]

uniprot summary :

XLKD1: Ligand-specific transporter trafficking between intracellular organelles (TGN) and the plasma membrane. Plays a role in autocrine regulation of cell growth mediated by growth regulators containing cell surface retention sequence binding (CRS). May act as an hyaluronan (HA) transporter, either mediating its uptake for catabolism within lymphatic endothelial cells themselves, or its transport into the lumen of afferent lymphatic vessels for subsequent re-uptake and degradation in lymph nodes. Protein type: Motility/polarity/chemotaxis; Membrane protein, integral. Chromosomal Location of Human Ortholog: 11p15. Cellular Component: integral to plasma membrane; membrane; plasma membrane. Molecular Function: hyaluronic acid binding; protein binding; receptor activity; transmembrane receptor activity. Biological Process: anatomical structure morphogenesis; carbohydrate metabolic process; cell motility; cell-matrix adhesion; glycosaminoglycan metabolic process; hyaluronan catabolic process; hyaluronan metabolic process; response to wounding; signal transduction; transport

size1 :

0.2 mL

price1 :

530 USD

more info or order :

MyBioSource product webpage