Anti-RAB7A / RAB7 Antibody (C-Terminus) IHC-plus | MyBioSource MBS249664 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Anti-RAB7A / RAB7 Antibody (C-Terminus) IHC-plus

catalog :

MBS249664

quantity :

0.1 mL

price :

530 USD

clonality :

polyclonal

host :

goat

conjugate :

nonconjugated

reactivity :

human, mouse, rat, dog

application :

western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS249664

products type :

Antibody

products full name :

Anti-RAB7A / RAB7 Antibody (C-Terminus) IHC-plus

products short name :

[RAB7A / RAB7]

products name syn :

[RAB7A; CMT2B; PRO2706; RAB7; Ras-associated protein RAB7; Ras-related protein Rab-7a; PSN]

other names :

[Ras-related protein Rab-7a; Ras-related protein Rab-7a; ras-related protein Rab-7a; RAB7A, member RAS oncogene family]

products gene name :

[RAB7A / RAB7]

other gene names :

[RAB7A; RAB7A; RAB7; PRO2706; RAB7]

uniprot entry name :

RAB7A_HUMAN

clonality :

Polyclonal

isotype :

IgG

host :

goat

reactivity :

Human, Monkey, Mouse, Rat , Dog

sequence length :

207

specificity :

Detects Rab7a protein in the human, rat and mouse whole cell lysates and transfected cells with GFP-Rab7a by Western blot. This Ab is specific for Rab7a.

purity :

Immunoaffinity purified

form :

PBS, 20% glycerol, 0.05% sodium azide.

concentration :

3 mg/mL

storage stability :

Short term: Store at 4°C ; Long term: Store at -20°C. Avoid freeze/thaw cycles.

tested application :

Immunohistochemistry (IHC) - Paraffin, Immunofluorescence (IF), Western Blot (WB)

app notes :

IHC- Paraffin (5 ug/mL). Immunofluorescence (1:50-1:250). Western Blot (1:250-1:2000). Optimal dilution to be determined by the researcher.

other info1 :

Immunogen: RAB7A / RAB7 antibody was raised against purified recombinant peptide derived from within residues 110 aa of the C-terminus of mouse Rab7a produced in E Coli. Immunogen Species: RAB7A/RAB7 antibody was raised against Mouse. Antigen Type: Recombinant protein. Epitope: C-terminus. Note: During shipment,small volumes of product will ocassionally become entrapped in the seal of the product vial. We recommend briefly centrifuging the vial to dislodge any liquid in the container's cap prior to opening. Warning: This reagent may contain sodium azide. The chemical, physical, and toxicologial properties of this material have not been thoughly investigated.Standard Laboratory Practices should be followed. Avoid skin and eye contact,inhalation, and ingestion. Sodium azide forms hydrazoic acid under acidic conditions and may react with lead or copper plubing to form highly explosive metal azides. On disposal, flush with large volumes of water to prevent accumulation.

other info2 :

. Disclaimer: Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application. Recommended Immunohistochemistry Protocol: The following protocol is a recommendation only, and MyBioSource, Inc. makes no guarantee of the results:. Tissue Preparation: . Formalin fixation and embedding in paraffin wax. Tissue Sectioning: . Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C. Deparaffinization: . Wash dry slides in 3 changes of xylene - 5 minutes each @ RT. Rehydration: . Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT. Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT. Wash slides in 1 change of 80% alcohol - 3 minutes @ RT. Rinse slides in gentle running distilled water - 5 minutes @ RT. Antigen retrieval: . Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes. Remove from heat and let stand at room temperature in buffer - 20 minutes. Rinse in 1X TBS with Tween (TBST) -1 minute @ RT. Immunostaining: . (Do not allow tissues to dry at any time during the staining procedure) . Apply a universal protein block - 20 minutes @ RT. Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT. Rinse slides in 1 X TBST - 1 minute @ RT. Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT. Rinse slides in 1X TBST -1 minute @ RT. Apply alkaline phosphatase streptavidin - 30 minutes @ RT. Rinse slides in 1X TBST -1 minute @ RT. Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT. Wash slides in distilled water - 1 minute @ RT. Dehydrate: . (This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB) . Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT. Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT. Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT. Wash slides in 3 changes of xylene - 1 minute each @ RT. Apply coverslip

products description :

RAB7A Antibody, CMT2B Antibody, PRO2706 Antibody, RAB7 Antibody, Ras-associated protein RAB7 Antibody, Ras-related protein Rab-7a Antibody, PSN Antibody Description: Key regulator in endo-lysosomal trafficking. Governs early-to-late endosomal maturation, microtubule minus-end as well as plus-end directed endosomal migration and positioning, and endosome-lysosome transport through different protein-protein interaction cascades.

ncbi gi num :

1709999

ncbi acc num :

P51149.1

uniprot acc num :

P51149

ncbi pathways :

Adaptive Immune System Pathway (1269171); Amoebiasis Pathway (167324); Amoebiasis Pathway (167191); Endocytosis Pathway (102279); Endocytosis Pathway (102181); IL12-mediated Signaling Events Pathway (137922); Immune System Pathway (1269170); MHC Class II Antigen Presentation Pathway (1269200); Phagosome Pathway (153910); Phagosome Pathway (153859)

ncbi summary :

RAB family members are small, RAS-related GTP-binding proteins that are important regulators of vesicular transport. Each RAB protein targets multiple proteins that act in exocytic / endocytic pathways. This gene encodes a RAB family member that regulates vesicle traffic in the late endosomes and also from late endosomes to lysosomes. This encoded protein is also involved in the cellular vacuolation of the VacA cytotoxin of Helicobacter pylori. Mutations at highly conserved amino acid residues in this gene have caused some forms of Charcot-Marie-Tooth (CMT) type 2 neuropathies. [provided by RefSeq, Jul 2008]

uniprot summary :

Rab7: a member of the RAB family of RAS-related GTP-binding proteins, important regulators of vesicular transport and are located in specific intracellular compartments. RAB7 has been localized to late endosomes and shown to be important in the late endocytic pathway. In addition, it has been shown to have a fundamental role in the cellular vacuolation induced by the cytotoxin VacA of Helicobacter pylori. Mutations in this protein cause Charcot-Marie-Tooth type 2B neuropathy. Protein type: G protein, monomeric, Rab; G protein, monomeric; G protein. Chromosomal Location of Human Ortholog: 3q21.3. Cellular Component: Golgi apparatus; intracellular membrane-bound organelle; late endosome; late endosome membrane; lipid particle; lysosomal membrane; lysosome; melanosome membrane; phagocytic vesicle; phagocytic vesicle membrane; pre-autophagosomal structure membrane; retromer complex; terminal button; vacuolar membrane. Molecular Function: GDP binding; GTP binding; GTPase activity; protein binding; Rac GTPase binding. Biological Process: antigen processing and presentation of exogenous peptide antigen via MHC class II; bone resorption; early endosome to late endosome transport; endocytosis; endosome to lysosome transport; epidermal growth factor ligand processing; positive regulation of protein catabolic process; positive regulation of viral reproduction; protein targeting to lysosome; protein to membrane docking; protein transport; release of virus from host; retrograde transport, endosome to Golgi; small GTPase mediated signal transduction. Disease: Charcot-marie-tooth Disease, Axonal, Type 2b

size1 :

0.1 mL

price1 :

530 USD

more info or order :

MyBioSource product webpage