Anti-CD4 Antibody (clone 5D9) IHC-plus | MyBioSource MBS249480 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Anti-CD4 Antibody (clone 5D9) IHC-plus

catalog :

MBS249480

quantity :

0.05 mL

price :

530 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

[5D9]

reactivity :

human

application :

western blot, immunohistochemistry, immunohistochemistry - paraffin section

more info or order :

MyBioSource product webpage

image

image 1 :

Anti-CD4 antibody IHC staining of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.

image 2 :

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD4 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD4.

image 3 :

Western blot of extracts (35 ug) from 9 different cell lines by using anti-CD4 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

product information

catalog number :

MBS249480

products type :

Antibody

products full name :

Anti-CD4 Antibody (clone 5D9) IHC-plus

products short name :

[CD4]

products name syn :

[Mouse Monoclonal [clone 5D9] (IgG2a) to Human CD4; Human CD4; CD4 antigen; CD4 receptor; CD4mut; CD4 antigen (p55); CD4 molecule]

other names :

[T-cell surface glycoprotein CD4 isoform 1; T-cell surface glycoprotein CD4; T-cell surface glycoprotein CD4; CD4 molecule; T-cell surface antigen T4/Leu-3; CD_antigen: CD4]

products gene name :

[CD4]

other gene names :

[CD4; CD4; CD4mut]

uniprot entry name :

CD4_HUMAN

clonality :

Monoclonal

isotype :

IgG2a

clone :

[5D9]

host :

mouse

reactivity :

Human, Monkey

sequence length :

458

specificity :

Human CD4

purity :

Protein A/G purified

form :

PBS, pH 7.3 ,0.02% sodium azide; 50% glycerol, 1% BSA

concentration :

1 mg/ml

storage stability :

Store at -20°C. Avoid freeze-thaw cycles.

tested application :

Immunohistochemistry (IHC),Immunohistochemistry (IHC) Paraffin, Western Blot (WB)

app notes :

IHC-P (10 ug/ml). WB (1:2000). Optimal dilution to be determined by the researcher.

image1 heading :

Immunohistochemistry (IHC)

image2 heading :

Western Blot (WB)

image3 heading :

Western Blot (WB)

other info1 :

Immunogen Species: CD4 antibody was raised against Human. Immunogen: CD4 antibody was raised against full length human recombinant protein of human CD4 (NP_000607) produced in HEK293T cell. Immunogen Description: Full length human recombinant protein of human CD4 (NP_000607) produced in HEK293T cell. Immunogen Type: Recombinant protein. Target: CD4. Target Species: Human

other info2 :

Disclaimer: Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application. Recommended Immunohistochemistry Protocol: The following protocol is a recommendation only, and MyBioSource, Inc. makes no guarantee of the results:. Tissue Preparation: . Formalin fixation and embedding in paraffin wax. Tissue Sectioning: . Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C. Deparaffinization: . Wash dry slides in 3 changes of xylene - 5 minutes each @ RT. Rehydration: . Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT. Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT. Wash slides in 1 change of 80% alcohol - 3 minutes @ RT. Rinse slides in gentle running distilled water - 5 minutes @ RT. Antigen retrieval: . Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes. Remove from heat and let stand at room temperature in buffer - 20 minutes. Rinse in 1X TBS with Tween (TBST) -1 minute @ RT. Immunostaining: . (Do not allow tissues to dry at any time during the staining procedure) . Apply a universal protein block - 20 minutes @ RT. Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT. Rinse slides in 1 X TBST - 1 minute @ RT. Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT. Rinse slides in 1X TBST -1 minute @ RT. Apply alkaline phosphatase streptavidin - 30 minutes @ RT. Rinse slides in 1X TBST -1 minute @ RT. Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT. Wash slides in distilled water - 1 minute @ RT. Dehydrate: . (This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB) . Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT. Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT. Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT. Wash slides in 3 changes of xylene - 1 minute each @ RT. Apply coverslip . Note: During shipment,small volumes of product will ocassionally become entrapped in the seal of the product vial. We recommend briefly centrifuging the vial to dislodge any liquid in the container's cap prior to opening. Warning: This reagent may contain sodium azide. The chemical, physical, and toxicologial properties of this material have not been thoughly investigated.Standard Laboratory Practices should be followed. Avoid skin and eye contact,inhalation, and ingestion. Sodium azide forms hydrazoic acid under acidic conditions and may react with lead or copper plubing to form highly explosive metal azides. On disposal, flush with large volumes of water to prevent accumulation.

products description :

CD4 Antibody, CD4 antigen Antibody, CD4 receptor Antibody, CD4mut Antibody, CD4 antigen (p55) Antibody, CD4 molecule Antibody Description: CD4 is a membrane glycoprotein of T lymphocytes that interacts with major histocompatibility complex class II antigenes and is also a receptor for the human immunodeficiency virus. This gene is expressed not only in T lymphocytes, but also in B cells, macrophages, and granulocytes. It is also expressed in specific regions of the brain.

ncbi gi num :

10835167

ncbi acc num :

NP_000607.1

ncbi gb acc num :

NM_000616.4

uniprot acc num :

P01730

ncbi mol weight :

51,111 Da

ncbi pathways :

Adaptive Immune System Pathway (1269171); Alpha-defensins Pathway (1269266); Antigen Processing And Presentation Pathway (83074); Antigen Processing And Presentation Pathway (485); Arf1 Pathway (137927); Binding And Entry Of HIV Virion Pathway (1269060); C-MYB Transcription Factor Network Pathway (138073); CXCR4-mediated Signaling Events Pathway (137910); Cell Adhesion Molecules (CAMs) Pathway (83069); Cell Adhesion Molecules (CAMs) Pathway (480)

ncbi summary :

This gene encodes a membrane glycoprotein of T lymphocytes that interacts with major histocompatibility complex class II antigenes and is also a receptor for the human immunodeficiency virus. This gene is expressed not only in T lymphocytes, but also in B cells, macrophages, and granulocytes. It is also expressed in specific regions of the brain. The protein functions to initiate or augment the early phase of T-cell activation, and may function as an important mediator of indirect neuronal damage in infectious and immune-mediated diseases of the central nervous system. Multiple alternatively spliced transcript variants encoding different isoforms have been identified in this gene. [provided by RefSeq, Aug 2010]

uniprot summary :

CD4: Accessory protein for MHC class-II antigen/T-cell receptor interaction. May regulate T-cell activation. Induces the aggregation of lipid rafts. Associates with LCK. Binds to HIV-1 gp120 and to P4HB/PDI and upon HIV-1 binding to the cell membrane, is part of P4HB/PDI- CD4-CXCR4-gp120 complex. Interacts with HIV-1 Envelope polyprotein gp160 and protein Vpu. Interacts with Human Herpes virus 7 capsid proteins. Interacts with PTK2/FAK1; this interaction requires the presence of HIV-1 gp120. Protein type: Membrane protein, integral; Cell surface. Chromosomal Location of Human Ortholog: 12p13.31. Cellular Component: early endosome; endoplasmic reticulum lumen; endoplasmic reticulum membrane; external side of plasma membrane; integral to membrane; lipid raft; plasma membrane; T cell receptor complex. Molecular Function: coreceptor activity; enzyme binding; extracellular matrix structural constituent; glycoprotein binding; immunoglobulin binding; MHC class II protein binding; protein binding; protein homodimerization activity; protein kinase binding; receptor activity; transmembrane receptor activity; viral receptor activity; zinc ion binding. Biological Process: adaptive immune response; cell surface receptor linked signal transduction; cytokine production; defense response to Gram-negative bacterium; entry into host cell; entry of virus into host cell; enzyme linked receptor protein signaling pathway; immune response; induction by virus of cell-cell fusion in host; innate immune response; maintenance of cellular protein localization; positive regulation of calcium-mediated signaling; positive regulation of interleukin-2 biosynthetic process; positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of protein kinase activity; positive regulation of T cell proliferation; regulation of defense response to virus by virus; regulation of T cell activation; response to estradiol stimulus; response to vitamin D; signal transduction; T cell costimulation; T cell differentiation; T cell receptor signaling pathway; T cell selection; transmembrane receptor protein tyrosine kinase signaling pathway; viral reproduction. Disease: Okt4 Epitope Deficiency

size1 :

0.05 mL

price1 :

530 USD

more info or order :

MyBioSource product webpage