Anti-CTSG / Cathepsin G Antibody (N-Terminus, clone 9i239) IHC-plus | MyBioSource MBS249442 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Anti-CTSG / Cathepsin G Antibody (N-Terminus, clone 9i239) IHC-plus

catalog :

MBS249442

quantity :

0.05 mL

price :

605 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

[9i239]

reactivity :

human

application :

western blot, immunohistochemistry, immunohistochemistry - paraffin section

more info or order :

MyBioSource product webpage

image

image 1 :

Anti-CTSG / Cathepsin G antibody IHC staining of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.

product information

catalog number :

MBS249442

products type :

Antibody

products full name :

Anti-CTSG / Cathepsin G Antibody (N-Terminus, clone 9i239) IHC-plus

products short name :

[CTSG / Cathepsin G]

products name syn :

[Mouse Monoclonal [clone 9i239] (IgG1) to Human CTSG / Cathepsin G; Human CTSG / Cathepsin G; CATG; Cathepsin G; CG]

other names :

[cathepsin G preproprotein; Cathepsin G; cathepsin G; cathepsin G]

products gene name :

[CTSG]

other gene names :

[CTSG; CTSG; CG; CATG; CG]

uniprot entry name :

CATG_HUMAN

clonality :

Monoclonal

isotype :

IgG1

clone :

[9i239]

host :

Mouse

reactivity :

Human

sequence length :

255

specificity :

Recognizes human Cathepsin G.

purity :

Unpurified Supernatant

form :

Tissue culture supernatant, 15 mM sodium azide

storage stability :

May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C.

tested application :

Immunohistochemistry (IHC) Paraffin, Western Blot (WB)

app notes :

IHC-P (1:100). WB (1:50). Optimal dilutions to be determined by the researcher.

image1 heading :

Immunohistochemistry (IHC)

other info1 :

Immunogen: CTSG/Cathepsin G antibody was raised against prokaryotic recombinant protein corresponding to a 124aa, N-terminal region of human cathepsin G molecule. Immunogen Description: Prokaryotic recombinant protein corresponding to a 124aa, N-terminal region of human cathepsin G molecule. Immunogen Type: Recombinant protein

other info2 :

Antigen Modification: N-Terminus. Target: CTSG/Cathepsin G. Family/Subfamily: Protease/Serine S1. Disclaimer: Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application. Recommended Immunohistochemistry Protocol: The following protocol is a recommendation only, and MyBioSource, Inc. makes no guarantee of the results:. Tissue Preparation: . Formalin fixation and embedding in paraffin wax. Tissue Sectioning: . Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C. Deparaffinization: . Wash dry slides in 3 changes of xylene - 5 minutes each @ RT. Rehydration: . Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT. Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT. Wash slides in 1 change of 80% alcohol - 3 minutes @ RT. Rinse slides in gentle running distilled water - 5 minutes @ RT. Antigen retrieval: . Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes. Remove from heat and let stand at room temperature in buffer - 20 minutes. Rinse in 1X TBS with Tween (TBST) -1 minute @ RT. Immunostaining: . (Do not allow tissues to dry at any time during the staining procedure) . Apply a universal protein block - 20 minutes @ RT. Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT. Rinse slides in 1 X TBST - 1 minute @ RT. Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT. Rinse slides in 1X TBST -1 minute @ RT. Apply alkaline phosphatase streptavidin - 30 minutes @ RT. Rinse slides in 1X TBST -1 minute @ RT. Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT. Wash slides in distilled water - 1 minute @ RT. Dehydrate: . (This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB) . Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT. Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT. Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT. Wash slides in 3 changes of xylene - 1 minute each @ RT. Apply coverslip

products description :

CTSG Antibody, CATG Antibody, Cathepsin G Antibody, CG Antibody Description: CTSG / Cathepsin G, a member of the peptidase S1 protein family, is found in azurophil granules of neutrophilic polymorphonuclear leukocytes. The encoded protease has a specificity similar to that of chymotrypsin C, and may participate in the killing and digestion of engulfed pathogens, and in connective tissue remodeling at sites of inflammation. In addition, the encoded protein is antimicrobial, with bacteriocidal activity against S. aureus and N. gonorrhoeae.

ncbi gi num :

4503149

ncbi acc num :

NP_001902.1

ncbi gb acc num :

NM_001911.2

uniprot acc num :

P08311

ncbi mol weight :

28,837 Da

ncbi pathways :

ACE Inhibitor Pathway (198763); Activation Of Matrix Metalloproteinases Pathway (1270258); Amoebiasis Pathway (167324); Amoebiasis Pathway (167191); Degradation Of The Extracellular Matrix Pathway (1270257); Extracellular Matrix Organization Pathway (1270244); Lysosome Pathway (99052); Lysosome Pathway (96865); Metabolism Of Angiotensinogen To Angiotensins Pathway (1268749); Metabolism Of Proteins Pathway (1268677)

ncbi summary :

The protein encoded by this gene, a member of the peptidase S1 protein family, is found in azurophil granules of neutrophilic polymorphonuclear leukocytes. The encoded protease has a specificity similar to that of chymotrypsin C, and may participate in the killing and digestion of engulfed pathogens, and in connective tissue remodeling at sites of inflammation. In addition, the encoded protein is antimicrobial, with bacteriocidal activity against S. aureus and N. gonorrhoeae. Transcript variants utilizing alternative polyadenylation signals exist for this gene. [provided by RefSeq, Sep 2014]

uniprot summary :

CTSG: Serine protease with trypsin- and chymotrypsin-like specificity. Cleaves complement C3. Has antibacterial activity against the Gram-negative bacterium P.aeruginosa, antibacterial activity is inhibited by LPS from P.aeruginosa, Z-Gly-Leu-Phe- CH2Cl and phenylmethylsulfonyl fluoride. Belongs to the peptidase S1 family. Protein type: Protease; EC 3.4.21.20; Motility/polarity/chemotaxis. Chromosomal Location of Human Ortholog: 14q11.2. Cellular Component: cell surface; extracellular matrix; extracellular region; extracellular space; nucleus; plasma membrane; secretory granule. Molecular Function: heparin binding; peptidase activity; protein binding; serine-type endopeptidase activity. Biological Process: angiotensin maturation; cellular protein metabolic process; defense response to fungus; extracellular matrix disassembly; extracellular matrix organization and biogenesis; immune response; positive regulation of immune response; protein amino acid phosphorylation; protein processing; proteolysis; response to lipopolysaccharide

size1 :

0.05 mL

price1 :

605 USD

more info or order :

MyBioSource product webpage