Anti-GIMAP4 Antibody (clone 1C6) IHC-plus | MyBioSource MBS249271 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Anti-GIMAP4 Antibody (clone 1C6) IHC-plus

catalog :

MBS249271

quantity :

0.05 mL

price :

530 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

clone name :

[1C6]

reactivity :

human

application :

western blot, immunohistochemistry, immunocytochemistry, flow cytometry, FACS, immunohistochemistry - paraffin section

more info or order :

MyBioSource product webpage

image

image 1 :

Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE)

image 2 :

Anti-GIMAP4 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY GIMAP4.

image 3 :

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GIMAP4 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GIMAP4.

product information

catalog number :

MBS249271

products type :

Antibody

products full name :

Anti-GIMAP4 Antibody (clone 1C6) IHC-plus

products short name :

[GIMAP4]

products name syn :

[Mouse Monoclonal [clone 1C6] (IgG2a) to Human GIMAP4; Human GIMAP4; GTPase IMAP family member 4; GTPase; IMAP family member 4; HIMAP4; IAN-1; IAN1; Immunity associated protein 4; Immunity-associated protein 4; MSTP062; HIAN1; IMAP4]

other names :

[GTPase IMAP family member 4; GTPase IMAP family member 4; GTPase IMAP family member 4; GTPase, IMAP family member 4; Immunity-associated nucleotide 1 protein; IAN-1; hIAN1; Immunity-associated protein 4]

products gene name :

[GIMAP4]

other gene names :

[GIMAP4; GIMAP4; IAN1; IAN-1; IMAP4; MSTP062; IAN1; IMAP4; IAN-1; hIAN1]

uniprot entry name :

GIMA4_HUMAN

clonality :

Polyclonal

isotype :

IgG2a

clone :

[1C6]

host :

Rabbit

reactivity :

Human

sequence length :

329

specificity :

Human GIMAP4

purity :

Protein A/G purified

form :

PBS, pH 7.3, 1% BSA, 50% glycerol, 0.02% sodium azide

storage stability :

Store at -20 degree C. Minimize freezing and thawing.

tested application :

Immunohistochemistry (IHC) Paraffin, Immunofluorescence (IF), Western Blot (WB), Flow Cytometry (FC/FACS)

app notes :

Flow Cytometry (1:100). IF (1:100). IHC-P (10 ug/ml). WB (1:2000). Optimal dilution tobe determined by the researcher.

image1 heading :

Immunohistochemistry (IHC)

image2 heading :

Immunofluorescence (IF)

image3 heading :

Western Blot (WB)

image4 heading :

Flow Cytometry (FC/FACS)

image4 description :

HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-GIMAP4 antibody, and then analyzed by flow cytometry.

other info1 :

Immunogen: GIMAP4 antibody was raised against full length human recombinant protein of human GIMAP4(NP_060796) produced in HEK293T cell. Immunogen Description: Full length human recombinant protein of human GIMAP4(NP_060796) produced in HEK293T cell. Immunogen Type: Recombinant protein

other info2 :

Target Species: Human. Disclaimer: Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application. Recommended Immunohistochemistry Protocol: The following protocol is a recommendation only, and MyBioSource, Inc. makes no guarantee of the results:. Tissue Preparation: . Formalin fixation and embedding in paraffin wax. Tissue Sectioning: . Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C. Deparaffinization: . Wash dry slides in 3 changes of xylene - 5 minutes each @ RT. Rehydration: . Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT. Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT. Wash slides in 1 change of 80% alcohol - 3 minutes @ RT. Rinse slides in gentle running distilled water - 5 minutes @ RT. Antigen retrieval: . Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes. Remove from heat and let stand at room temperature in buffer - 20 minutes. Rinse in 1X TBS with Tween (TBST) -1 minute @ RT. Immunostaining: . (Do not allow tissues to dry at any time during the staining procedure) . Apply a universal protein block - 20 minutes @ RT. Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT. Rinse slides in 1 X TBST - 1 minute @ RT. Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT. Rinse slides in 1X TBST -1 minute @ RT. Apply alkaline phosphatase streptavidin - 30 minutes @ RT. Rinse slides in 1X TBST -1 minute @ RT. Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT. Wash slides in distilled water - 1 minute @ RT. Dehydrate: . (This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB) . Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT. Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT. Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT. Wash slides in 3 changes of xylene - 1 minute each @ RT. Apply coverslip

products description :

GIMAP4 Antibody, GTPase IMAP family member 4 Antibody, GTPase, IMAP family member 4 Antibody, HIMAP4 Antibody, IAN-1 Antibody, IAN1 Antibody, Immunity associated protein 4 Antibody, Immunity-associated protein 4 Antibody, MSTP062 Antibody, HIAN1 Antibody, IMAP4 Antibody Description: May play a role in regulating lymphocyte apoptosis (By similarity). Exhibits intrisinic GTPase activity. Shows a higher affinity for GDP over GTP (about 12-fold higher), and binding shows an absolute requirement for magnesium.

ncbi gi num :

8922873

ncbi acc num :

NP_060796.1

ncbi gb acc num :

NM_018326.2

uniprot acc num :

Q9NUV9

ncbi mol weight :

37,534 Da

ncbi summary :

This gene encodes a protein belonging to the GTP-binding superfamily and to the immuno-associated nucleotide (IAN) subfamily of nucleotide-binding proteins. The encoded protein of this gene may be negatively regulated by T-cell acute lymphocytic leukemia 1 (TAL1). In humans, the IAN subfamily genes are located in a cluster at 7q36.1. [provided by RefSeq, Jul 2008]

uniprot summary :

GIMAP4: May play a role in regulating lymphocyte apoptosis. Exhibits intrisinic GTPase activity. Shows a higher affinity for GDP over GTP (about 12-fold higher), and binding shows an absolute requirement for magnesium. Belongs to the IAN GTP-binding protein family. Protein type: Hydrolase. Chromosomal Location of Human Ortholog: 7q36.1. Cellular Component: cytosol. Molecular Function: GTP binding

size1 :

0.05 mL

price1 :

530 USD

more info or order :

MyBioSource product webpage