Anti-IDS Antibody (clone 3B10) IHC-plus | MyBioSource MBS249242 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Anti-IDS Antibody (clone 3B10) IHC-plus

catalog :

MBS249242

quantity :

0.05 mL

price :

530 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

[3B10]

reactivity :

human

application :

western blot, immunohistochemistry, immunocytochemistry, flow cytometry, FACS, immunohistochemistry - paraffin section

more info or order :

MyBioSource product webpage

image

image 1 :

Human Brain, Cortex: Formalin-Fixed, Paraffin-Embedded (FFPE)

image 2 :

Anti-IDS mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY IDS.

image 3 :

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IDS (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IDS.

product information

catalog number :

MBS249242

products type :

Antibody

products full name :

Anti-IDS Antibody (clone 3B10) IHC-plus

products short name :

[IDS]

products name syn :

[Mouse Monoclonal [clone 3B10] (IgG2a) to Human IDS; Human IDS; Idursulfase; SIDS; Iduronate 2-sulfatase; MPS2]

other names :

[iduronate 2-sulfatase isoform a preproprotein; Iduronate 2-sulfatase; iduronate 2-sulfatase; iduronate 2-sulfatase; Alpha-L-iduronate sulfate sulfatase; Idursulfase]

products gene name :

[IDS]

other gene names :

[IDS; IDS; MPS2; SIDS; SIDS; Idursulfase]

uniprot entry name :

IDS_HUMAN

clonality :

Monoclonal

isotype :

IgG2a

clone :

[3B10]

host :

mouse

reactivity :

Human

sequence length :

550

specificity :

Human IDS

purity :

Protein A/G purified

form :

PBS, pH 7.3, 0.02% Sodium Azide, 50% Glycerol, 1% BSA.

concentration :

0.79 mg/ml

storage stability :

Store at -20°C. Minimize freezing and thawing.

tested application :

Immunohistochemistry (IHC) Paraffin, Immunofluorescence (IF), Western Blot (WB), Flow Cytometry (FC/FACS)

app notes :

Flow Cytometry (1:100). IF (1:100). IHC-P (10 ug/ml). WB (1:2000)

image1 heading :

Immunohistochemistry - Paraffin (IHC)

image2 heading :

Immunofluorescence (IF)

image3 heading :

Western Blot (WB)

image4 heading :

Flow Cytometry (FC/FACS)

image4 description :

Flow cytometry of HeLa cells, using anti-IDS antibody (Red), compared to a nonspecific negative control antibody (Blue).

image5 heading :

Flow Cytometry (FC/FACS)

image5 description :

Flow cytometry of Jurkat cells, using anti-IDS antibody (Red), compared to a nonspecific negative control antibody (Blue).

other info1 :

Immunogen: IDS/Iduronate 2 Sulfatase atnibody was raised against full length human recombinant protein of human IDS (NP_000193) produced in HEK293T cell. Target: IDS/Iduronate 2 Sulfatase.

other info2 :

Disclaimer: Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application. Recommended Immunohistochemistry Protocol: The following protocol is a recommendation only, and MyBioSource, Inc. makes no guarantee of the results:. Tissue Preparation: . Formalin fixation and embedding in paraffin wax. Tissue Sectioning: . Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C. Deparaffinization: . Wash dry slides in 3 changes of xylene - 5 minutes each @ RT. Rehydration: . Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT. Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT. Wash slides in 1 change of 80% alcohol - 3 minutes @ RT. Rinse slides in gentle running distilled water - 5 minutes @ RT. Antigen retrieval: . Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes. Remove from heat and let stand at room temperature in buffer - 20 minutes. Rinse in 1X TBS with Tween (TBST) -1 minute @ RT. Immunostaining: . (Do not allow tissues to dry at any time during the staining procedure) . Apply a universal protein block - 20 minutes @ RT. Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT. Rinse slides in 1 X TBST - 1 minute @ RT. Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT. Rinse slides in 1X TBST -1 minute @ RT. Apply alkaline phosphatase streptavidin - 30 minutes @ RT. Rinse slides in 1X TBST -1 minute @ RT. Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT. Wash slides in distilled water - 1 minute @ RT. Dehydrate: . (This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB) . Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT. Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT. Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT. Wash slides in 3 changes of xylene - 1 minute each @ RT. Apply coverslip . Note: During shipment,small volumes of product will ocassionally become entrapped in the seal of the product vial. We recommend briefly centrifuging the vial to dislodge any liquid in the container's cap prior to opening. Warning: This reagent may contain sodium azide. The chemical, physical, and toxicologial properties of this material have not been thoughly investigated.Standard Laboratory Practices should be followed. Avoid skin and eye contact,inhalation, and ingestion. Sodium azide forms hydrazoic acid under acidic conditions and may react with lead or copper plubing to form highly explosive metal azides. On disposal, flush with large volumes of water to prevent accumulation.

products description :

IDS Antibody, Idursulfase Antibody, SIDS Antibody, Iduronate 2-sulfatase Antibody, MPS2 Antibody Description: Iduronate-2-sulfatase is required for the lysosomal degradation of heparan sulfate and dermatan sulfate. Mutations in this X-chromosome gene that result in enzymatic deficiency lead to the sex-linked Mucopolysaccharidosis Type II, also known as Hunter Syndrome. Iduronate-2-sulfatase has a strong sequence homology with human arylsulfatases A, B, and C, and human glucosamine-6-sulfatase. A splice variant of this gene has been described.

ncbi gi num :

4557659

ncbi acc num :

NP_000193.1

ncbi gb acc num :

NM_000202.7

uniprot acc num :

P22304

ncbi pathways :

CS/DS Degradation Pathway (1269987); Chondroitin Sulfate/dermatan Sulfate Metabolism Pathway (1269984); Dermatan Sulfate Degradation Pathway (413375); Dermatan Sulfate Degradation Pathway (468240); Glycosaminoglycan Degradation Pathway (82981); Glycosaminoglycan Degradation Pathway (355); Glycosaminoglycan Metabolism Pathway (1269972); HS-GAG Degradation Pathway (1269983); Heparan Sulfate Degradation Pathway (413377); Heparan Sulfate Degradation Pathway (468270)

ncbi summary :

This gene encodes a member of the sulfatase family of proteins. The encoded preproprotein is proteolytically processed to generate two polypeptide chains. This enzyme is involved in the lysosomal degradation of heparan sulfate and dermatan sulfate. Mutations in this gene are associated with the X-linked lysosomal storage disease mucopolysaccharidosis type II, also known as Hunter syndrome. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed. [provided by RefSeq, Jan 2016]

uniprot summary :

IDS: Required for the lysosomal degradation of heparan sulfate and dermatan sulfate. Defects in IDS are the cause of mucopolysaccharidosis type 2 (MPS2); also known as Hunter syndrome. MPS2 is an X-linked lysosomal storage disease characterized by intracellular accumulation of heparan sulfate and dermatan sulfate and their excretion in urine. Most children with MPS2 have a severe form with early somatic abnormalities including skeletal deformities, hepatosplenomegaly, and progressive cardiopulmonary deterioration. A prominent feature is neurological damage that presents as developmental delay and hyperactivity but progresses to mental retardation and dementia. They die before 15 years of age, usually as a result of obstructive airway disease or cardiac failure. In contrast, those with a mild form of MPS2 may survive into adulthood, with attenuated somatic complications and often without mental retardation. Belongs to the sulfatase family. 3 isoforms of the human protein are produced by alternative splicing. Protein type: EC 3.1.6.13; Hydrolase; Glycan Metabolism - glycosaminoglycan degradation. Chromosomal Location of Human Ortholog: Xq28. Cellular Component: lysosomal lumen. Molecular Function: iduronate-2-sulfatase activity; metal ion binding. Biological Process: carbohydrate metabolic process; chondroitin sulfate catabolic process; chondroitin sulfate metabolic process; glycosaminoglycan catabolic process; glycosaminoglycan metabolic process. Disease: Mucopolysaccharidosis, Type Ii

size1 :

0.05 mL

price1 :

530 USD

more info or order :

MyBioSource product webpage