Mouse Monoclonal [clone MN-1] (IgG) to Human MSLN / Mesothelin | MyBioSource MBS249172 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Mouse Monoclonal [clone MN-1] (IgG) to Human MSLN / Mesothelin

catalog :

MBS249172

quantity :

0.05 mg

price :

595 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

[MN-1]

reactivity :

human

application :

western blot, ELISA, immunohistochemistry, enzyme immunoassay

more info or order :

MyBioSource product webpage

image

image 1 :

Anti-MSLN / Mesothelin antibody IHC staining of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 15 ug/ml.

image 2 :

Anti-Mesothelin Antibodies - Immunohistochemistry. Immunohistochemistry using anti-mesothelin antibodies to detect mesothelin in PEFF human tissue sections treated by antigen retrieval methods. Anti-mesothelin primary antibodies were used to label these sections as follows: C, MAb MB; and D, MAb MN. Reprinted with permission fromClin. Cancer Res. 11(16):5840-6.

image 3 :

product information

catalog number :

MBS249172

products type :

Antibody

products full name :

Mouse Monoclonal [clone MN-1] (IgG) to Human MSLN / Mesothelin

products short name :

[MSLN / Mesothelin]

products name syn :

[Anti-MSLN / Mesothelin Antibody (Extracellular Domain, clone MN-1) IHC-plus; MSLN; CAK1 antigen; MPF; SMRP; Mesothelin; CAK1; Human MSLN; Mesothelin]

other names :

[mesothelin isoform 2 preproprotein; Mesothelin; mesothelin; CAK1 antigen; megakaryocyte potentiating factor; soluble MPF mesothelin related protein; pre-pro-megakaryocyte-potentiating factor; mesothelin; CAK1 antigen; Pre-pro-megakaryocyte-potentiating factorCleaved into the following 2 chains:Megakaryocyte-potentiating factor; MPF; Mesothelin, cleaved form]

products gene name :

[MSLN]

other gene names :

[MSLN; MSLN; MPF; SMRP; MPF; MPF]

uniprot entry name :

MSLN_HUMAN

clonality :

Monoclonal

isotype :

IgG

clone :

[MN-1]

host :

Mouse

reactivity :

Human

sequence length :

630

specificity :

Cross reactivity with homologues from other sources has not been tested.

purity :

Protein A Purified

form :

0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide, sterile filtered

concentration :

1 mg/ml

storage stability :

Short term 4 degree C, long term aliquot and store at -20 degree C, avoid freeze thaw cycles.

tested application :

Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)

app notes :

ELISA (1:10000 - 1:50000), IHC-P (15 ug/ml), WB (1:1000). Optimal dilution to be determined by the researcher.

image1 heading :

Immunohistochemistry (IHC)

image2 heading :

Immunohistochemistry (IHC)

image3 heading :

Western Blot (WB)

other info1 :

Target Species: Human. Immunogen Description: This antibody was produced in mice by repeated immunizations with a recombinant protein corresponding to the extracellular domain of human mesothelin. Usage: This antibody has been tested for use in immunohistochemistry and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 40 kD in size corresponding to mature mesothelin by western blotting in the appropriate cell lysate or extract. For immunohistochemistry, archival PEFF human tissues were deparaffinized followed by hydration. Antigen-retrieval is recommended. Block tissues with 1% BSA in PBS for 30 min at 23 degree C. Antibodies are diluted in 1% BSA and reacted with tissue for 60 min at room temperature.

other info2 :

Immunogen Species: MSLN/Mesothelin antibody was raised against Human. Immunogen Type: Recombinant protein. Immunogen: MSLN / Mesothelin antibody was raised against this antibody was produced in mice by repeated immunizations with a recombinant protein corresponding to the extracellular domain of human mesothelin. Antigen Modification: Extracellular Domain. Disclaimer: Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application. Recommended Immunohistochemistry Protocol: The following protocol is a recommendation only, and MyBioSource, Inc. makes no guarantee of the results:. Tissue Preparation: . Formalin fixation and embedding in paraffin wax. Tissue Sectioning: . Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C. Deparaffinization: . Wash dry slides in 3 changes of xylene - 5 minutes each @ RT. Rehydration: . Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT. Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT. Wash slides in 1 change of 80% alcohol - 3 minutes @ RT. Rinse slides in gentle running distilled water - 5 minutes @ RT. Antigen retrieval: . Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes. Remove from heat and let stand at room temperature in buffer - 20 minutes. Rinse in 1X TBS with Tween (TBST) -1 minute @ RT. Immunostaining: . (Do not allow tissues to dry at any time during the staining procedure) . Apply a universal protein block - 20 minutes @ RT. Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT. Rinse slides in 1 X TBST - 1 minute @ RT. Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT. Rinse slides in 1X TBST -1 minute @ RT. Apply alkaline phosphatase streptavidin - 30 minutes @ RT. Rinse slides in 1X TBST -1 minute @ RT. Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT. Wash slides in distilled water - 1 minute @ RT. Dehydrate: . (This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB) . Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT. Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT. Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT. Wash slides in 3 changes of xylene - 1 minute each @ RT. Apply coverslip

products description :

This gene encodes a preproprotein that is cleaved into two products, megakaryocyte potentiating factor and mesothelin. Alternative splicing results in multiple transcript variants. Mesothelin is a cell surface differentiation antigen found in mesothelial cells, mesotheliomas and ovarian cancers. Although the function of mesothelin is unknown, it may play a role in cellular adhesion. Three transcript variants have been described.

ncbi gi num :

53988380

ncbi acc num :

NP_037536.2

ncbi gb acc num :

NM_013404.4

uniprot acc num :

Q13421

ncbi mol weight :

67,940 Da

ncbi summary :

This gene encodes a precursor protein that is cleaved into two products, megakaryocyte potentiating factor and mesothelin. Megakaryocyte potentiation factor functions as a cytokine that can stimulate colony formation in bone marrow megakaryocytes. Mesothelian is a glycosylphosphatidylinositol-anchored cell-surface protein that may function as a cell adhesion protein. This protein is overexpressed in epithelial mesotheliomas, ovarian cancers and in specific squamous cell carcinomas. Alternative splicing results in multiple transcript variants.[provided by RefSeq, Apr 2010]

uniprot summary :

MSLN: Membrane-anchored forms may play a role in cellular adhesion. Antibodies against MSLN are detected in patients with mesothelioma and ovarian cancer. Belongs to the mesothelin family. 4 isoforms of the human protein are produced by alternative splicing. Protein type: Cell adhesion; Membrane protein, GPI anchor. Chromosomal Location of Human Ortholog: 16p13.3. Cellular Component: Golgi apparatus; extracellular space; cell surface; membrane; plasma membrane. Molecular Function: protein binding. Biological Process: pancreas development; cell adhesion

size1 :

0.05 mg

price1 :

595 USD

more info or order :

MyBioSource product webpage