product summary
Loading...
company name :
MyBioSource
product type :
antibody
product name :
Mouse Monoclonal [clone M1709Hg2] (IgG1) to Human Hemoglobin
catalog :
MBS249145
quantity :
0.25 mg
price :
530 USD
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
[M1709Hg2]
reactivity :
maize
application :
ELISA, immunohistochemistry, enzyme immunoassay
more info or order :
MyBioSource product webpage
image
image 1 :
MyBioSource MBS249145 image 1
Anti-Hemoglobin antibody IHC staining of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
product information
catalog number :
MBS249145
products type :
Antibody
products full name :
Mouse Monoclonal [clone M1709Hg2] (IgG1) to Human Hemoglobin
products short name :
[Hemoglobin]
products name syn :
[Anti-Hemoglobin Antibody (clone M1709Hg2) IHC-plus; Hemoglobin; Human Hemoglobin]
other names :
[hemoglobin; hemoglobin; GRMZM2G067402; non-symbiotic hemoglobin; reversible oxygen binding]
other gene names :
[LOC541815]
clonality :
Monoclonal
isotype :
IgG1
clone :
[M1709Hg2]
host :
Mouse
reactivity :
Human
sequence length :
165
specificity :
Human Hemoglobin
purity :
Ion exchange chromatography
form :
50 mM NaCl, 10mM PBS (pH 8.0) with 0.1% sodium azide
concentration :
1 mg/ml
storage stability :
Short term 4 degree C, long term aliquot and store at -20 degree C, avoid freeze thaw cycles.
tested application :
Immunohistochemistry (IHC - Paraffin), ELISA (EIA), LateralFlow
app notes :
ELISA, IHC-P (10 ug/ml), LateralFlow. Optimal dilution to be determined by the researcher.
image1 heading :
Immunohistochemistry (IHC)
other info1 :
Immunogen Species: Hemoglobin antibody was raised against Human. Immunogen Description: Human hemoglobin. Target: hemoglobin
other info2 :
Antigen Type: Purified protein. Immunogen: Hemoglobin antibody was raised against human hemoglobin. Disclaimer: Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application. Recommended Immunohistochemistry Protocol: The following protocol is a recommendation only, and MyBioSource, Inc. makes no guarantee of the results:. Tissue Preparation: . Formalin fixation and embedding in paraffin wax. Tissue Sectioning: . Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C. Deparaffinization: . Wash dry slides in 3 changes of xylene - 5 minutes each @ RT. Rehydration: . Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT. Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT. Wash slides in 1 change of 80% alcohol - 3 minutes @ RT. Rinse slides in gentle running distilled water - 5 minutes @ RT. Antigen retrieval: . Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes. Remove from heat and let stand at room temperature in buffer - 20 minutes. Rinse in 1X TBS with Tween (TBST) -1 minute @ RT. Immunostaining: . (Do not allow tissues to dry at any time during the staining procedure) . Apply a universal protein block - 20 minutes @ RT. Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT. Rinse slides in 1 X TBST - 1 minute @ RT. Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT. Rinse slides in 1X TBST -1 minute @ RT. Apply alkaline phosphatase streptavidin - 30 minutes @ RT. Rinse slides in 1X TBST -1 minute @ RT. Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT. Wash slides in distilled water - 1 minute @ RT. Dehydrate: . (This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB) . Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT. Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT. Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT. Wash slides in 3 changes of xylene - 1 minute each @ RT. Apply coverslip
ncbi gi num :
162462053
ncbi acc num :
NP_001104966.1
ncbi gb acc num :
NM_001111496.1
size1 :
0.25 mg
price1 :
530 USD
more info or order :
MyBioSource product webpage
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com
1-888-627-0165
headquarters: USA
MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.

"MyBioSource's number 1 vision is to be the world's number 1 quality reagents provider."

Our goal is to provide researchers, scientists and customers alike with a one-stop-shop for all of their reagents needs, whether it is monoclonal antibody, polyclonal antibody, recombinant protein, peptide, etc...

"MyBioSource offers the best products at unbeatable prices."

Please spend a few minutes to browse our online catalogs and see the wide range of products available. We ship our products through our shipping/distribution facility in San Diego, California, USA.

Would you like to receive email and e-newsletter from MyBioSource about new products, special offers and events? Please click here to join our Mailing List!