Mouse Monoclonal [clone 1H6] (IgG1) to Human PECAM-1 / CD31 | MyBioSource MBS249056 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Mouse Monoclonal [clone 1H6] (IgG1) to Human PECAM-1 / CD31

catalog :

MBS249056

quantity :

0.05 mL

price :

530 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

[1H6]

reactivity :

human, dog

application :

western blot, immunohistochemistry, immunocytochemistry, flow cytometry, FACS

more info or order :

MyBioSource product webpage

image

image 1 :

Anti-PECAM-1 / CD31 antibody IHC staining of human uterus, endometrium. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 20 ug/ml.

image 2 :

Anti-PECAM1 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PECAM1.

image 3 :

HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-PECAM1 antibody, and then analyzed by flow cytometry.

product information

catalog number :

MBS249056

products type :

Antibody

products full name :

Mouse Monoclonal [clone 1H6] (IgG1) to Human PECAM-1 / CD31

products short name :

[PECAM-1 / CD31]

products name syn :

[PECAM1; CD31; CD31 antigen; EndoCAM; PECA1; PECAM-1; GPIIA]

other names :

[platelet endothelial cell adhesion molecule; Platelet endothelial cell adhesion molecule; platelet endothelial cell adhesion molecule; CD31 antigen; platelet/endothelial cell adhesion molecule 1; EndoCAM; GPIIA'; PECA1; CD_antigen: CD31]

products gene name :

[PECAM-1]

products gene name syn :

[CD31]

other gene names :

[PECAM1; PECAM1; CD31; PECA1; GPIIA'; PECAM-1; endoCAM; CD31/EndoCAM; PECAM-1]

uniprot entry name :

PECA1_HUMAN

clonality :

Monoclonal

isotype :

IgG1

clone :

[1H6]

host :

Mouse

reactivity :

Human, Dog

specificity :

Human CD31

purity :

Immunoaffinity purified from tissue culture supernatant

form :

PBS, pH 7.3, 0.02% Sodium Azide, 50% Glycerol, 1% BSA

concentration :

1.2 mg/ml

storage stability :

Store at -20°C. Avoid freeze-thaw cycles.

tested application :

Immunohistochemistry - Paraffin (IHC), Immunofluorescence (IF), Western Blot (WB), Flow Cytometry (FC/FACS)

app notes :

IHC - Paraffin (20 ug/ml). Immunofluorescence (1:100). Western Blot (1:500 - 1:2000). Flow Cytometry (1:100). (Optimal dilution to be determined by the researcher.)

image1 heading :

Immunohistochemistry (IHC)

image2 heading :

Immunofluorescence (IF)

image3 heading :

Flow Cytometry (FC/FACS)

image4 heading :

Western Blot (WB)

image4 description :

Western blot of extracts (35 ug) from 9 different cell lines by using g anti-PECAM1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

image5 heading :

Western Blot (WB)

image5 description :

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PECAM1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PECAM1.

other info1 :

Immunogen Species: PECAM-1 / CD31 antibody was raised against Human.

other info2 :

. Disclaimer: Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application. Recommended Immunohistochemistry Protocol: The following protocol is a recommendation only, and MyBioSource, Inc. makes no guarantee of the results:. Tissue Preparation: . Formalin fixation and embedding in paraffin wax. Tissue Sectioning: . Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C. Deparaffinization: . Wash dry slides in 3 changes of xylene - 5 minutes each @ RT. Rehydration: . Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT. Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT. Wash slides in 1 change of 80% alcohol - 3 minutes @ RT. Rinse slides in gentle running distilled water - 5 minutes @ RT. Antigen retrieval: . Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes. Remove from heat and let stand at room temperature in buffer - 20 minutes. Rinse in 1X TBS with Tween (TBST) -1 minute @ RT. Immunostaining: . (Do not allow tissues to dry at any time during the staining procedure) . Apply a universal protein block - 20 minutes @ RT. Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT. Rinse slides in 1 X TBST - 1 minute @ RT. Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT. Rinse slides in 1X TBST -1 minute @ RT. Apply alkaline phosphatase streptavidin - 30 minutes @ RT. Rinse slides in 1X TBST -1 minute @ RT. Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT. Wash slides in distilled water - 1 minute @ RT. Dehydrate: . (This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB) . Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT. Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT. Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT. Wash slides in 3 changes of xylene - 1 minute each @ RT. Apply coverslip.

products description :

CD31 (PECAM1) is found on the surface of platelets, monocytes, neutrophils, and some types of T-cells, and makes up a large portion of endothelial cell intercellular junctions. CD31 is a member of the immunoglobulin superfamily and is likely involved in leukocyte migration, angiogenesis, and integrin activation.

ncbi gi num :

110347451

ncbi acc num :

NP_000433.3

ncbi gb acc num :

NM_000442.3

uniprot acc num :

P16284

ncbi pathways :

Cell Adhesion Molecules (CAMs) Pathway (83069); Cell Adhesion Molecules (CAMs) Pathway (480); Cell Surface Interactions At The Vascular Wall Pathway (106062); Extracellular Matrix Organization Pathway (576262); Hemostasis Pathway (106028); Integrin Cell Surface Interactions Pathway (106110); Leukocyte Transendothelial Migration Pathway (83083); Leukocyte Transendothelial Migration Pathway (494); Malaria Pathway (152665); Malaria Pathway (152657)

ncbi summary :

The protein encoded by this gene is found on the surface of platelets, monocytes, neutrophils, and some types of T-cells, and makes up a large portion of endothelial cell intercellular junctions. The encoded protein is a member of the immunoglobulin superfamily and is likely involved in leukocyte migration, angiogenesis, and integrin activation. [provided by RefSeq, May 2010]

uniprot summary :

PECAM-1: a cell adhesion molecule expressed on platelets and at endothelial cell intercellular junctions. Plays a role in modulation of integrin-mediated cell adhesion, transendothelial migration, angiogenesis, apoptosis, cell migration, negative regulation of immune cell signaling, autoimmunity, macrophage phagocytosis, IgE-mediated anaphylaxis and thrombosis. Undergoes alternative splicing, generating multiple isoforms in vascular beds of various tissues. Protein type: Membrane protein, integral; Cell adhesion. Chromosomal Location of Human Ortholog: 17q23.3. Cellular Component: extracellular space; platelet alpha granule membrane; integral to membrane; plasma membrane; cell junction. Molecular Function: protein binding. Biological Process: platelet activation; extracellular matrix organization and biogenesis; platelet degranulation; diapedesis; cell recognition; blood coagulation; signal transduction; cell adhesion; leukocyte migration; phagocytosis

size1 :

0.05 mL

price1 :

530 USD

more info or order :

MyBioSource product webpage