Goat Polyclonal (IgG) to Rat GJA1 / CX43 / Connexin 43 | MyBioSource MBS248943 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Goat Polyclonal (IgG) to Rat GJA1 / CX43 / Connexin 43

catalog :

MBS248943

quantity :

0.05 mL

price :

530 USD

clonality :

polyclonal

host :

goat

conjugate :

nonconjugated

reactivity :

human, mouse, rat, dog

application :

immunohistochemistry

more info or order :

MyBioSource product webpage

image

image 1 :

Anti-GJA1 / CX43 / Connexin 43 antibody IHC staining of human uterus, endometrium. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.

image 2 :

Immunofluorescence. Immunostaining of primary RPE cells with CX43 antibody at 1:100 dilution.

image 3 :

Western blot. Endogenous Cx43 detected with GJA1 / CX43 / Connexin 43 antibody at 1:1000 dilution. Lysate at 100 ug per lane and rabbit polyclonal to goat IgG (HRP) at 1:10000 dilution.

product information

catalog number :

MBS248943

products type :

Antibody

products full name :

Goat Polyclonal (IgG) to Rat GJA1 / CX43 / Connexin 43

products short name :

[GJA1 / CX43 / Connexin 43]

products name syn :

[Anti-GJA1 / CX43 / Connexin 43 Antibody (C-Terminus) IHC-plus; GJA1; Connexin-43; DFNB38; Connexin 43; Gap junction alpha-1 protein; GJAL; HSS; Gap junction protein alpha 1; ODDD; ODD; AVSD3; CX43; HLHS1; ODOD; SDTY3; Rat GJA1; CX43; Connexin 43]

other names :

[gap junction alpha-1 protein; Gap junction alpha-1 protein; gap junction alpha-1 protein; connexin 43; connexin-43; gap junction 43 kDa heart protein; gap junction protein, alpha 1, 43kDa; Connexin-43; Cx43; Gap junction 43 kDa heart protein]

products gene name :

[GJA1]

other gene names :

[GJA1; GJA1; HSS; CMDR; CX43; GJAL; ODDD; AVSD3; HLHS1; GJAL; Cx43]

uniprot entry name :

CXA1_HUMAN

clonality :

polyclonal

isotype :

IgG

host :

goat

reactivity :

Dog, Human, Monkey, Mouse, Rat

sequence length :

382

specificity :

Detects endogenous levels of total connexin 43.

purity :

Immunoaffinity Purified

form :

PBS, 0.05% Sodium Azide, 20% Glycerol

concentration :

4 mg/ml

storage stability :

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

tested application :

Immunohistochemistry (IHC)

app notes :

IHC-Paraffin (10 ug/mL). Immunofluorescence (1:50-1:250). Western Blot(1:500-1:2000). Optimal dilution to be determined by the researcher.

image1 heading :

Immunohistochemistry (IHC)

image2 heading :

Immunofluorescence (IF)

image3 heading :

Western Blot (WB)

other info1 :

Antigen Type: Recombinant protein. Epitope: C-terminus. Target Species: CJA1/CX43/Connexin 43. Immunogen Species: GJA1/CX43/Connexin 43 antibody was raised against Rat. Immunogen: GJA1/CX43/Connexin 43 antibody was raised against purified recombinant peptide derived from within residues 230 aa to the C-terminus of rat CX43 produced in E.coli.

other info2 :

Note: During shipment,small volumes of product will ocassionally become entrapped in the seal of the product vial. We recommend briefly centrifuging the vial to dislodge any liquid in the container's cap prior to opening. Warning: This reagent may contain sodium azide. The chemical, physical, and toxicologial properties of this material have not been thoughly investigated.Standard Laboratory Practices should be followed. Avoid skin and eye contact,inhalation, and ingestion. Sodium azide forms hydrazoic acid under acidic conditions and may react with lead or copper plubing to form highly explosive metal azides. On disposal, flush with large volumes of water to prevent accumulation. Disclaimer: Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect t this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application. Recommended Immunohistochemistry Protocol: The following protocol is a recommendation only, and MyBioSource, Inc. makes no guarantee of the results:. Tissue Preparation: . Formalin fixation and embedding in paraffin wax. Tissue Sectioning: . Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C. Deparaffinization: . Wash dry slides in 3 changes of xylene - 5 minutes each @ RT. Rehydration: . Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT. Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT. Wash slides in 1 change of 80% alcohol - 3 minutes @ RT. Rinse slides in gentle running distilled water - 5 minutes @ RT. Antigen retrieval: . Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes. Remove from heat and let stand at room temperature in buffer - 20 minutes. Rinse in 1X TBS with Tween (TBST) -1 minute @ RT. Immunostaining: . (Do not allow tissues to dry at any time during the staining procedure) . Apply a universal protein block - 20 minutes @ RT. Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT. Rinse slides in 1 X TBST - 1 minute @ RT. Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT. Rinse slides in 1X TBST -1 minute @ RT. Apply alkaline phosphatase streptavidin - 30 minutes @ RT. Rinse slides in 1X TBST -1 minute @ RT. Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT. Wash slides in distilled water - 1 minute @ RT. Dehydrate: . (This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB) . Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT. Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT. Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT. Wash slides in 3 changes of xylene - 1 minute each @ RT. Apply coverslip

products categories :

Family: Ion Channel. Subfamily: Connexin

products description :

Gap junction protein, alpha 1 is a member of the connexin gene family and a component of gap junctions. Gap junctions are composed of arrays of intercellular channels and provide a route for the diffusion of materials of low molecular weight from cell to cell. Connexin 43 is the major protein of gap junctions in the heart, and gap junctions are thought to have a crucial role in the synchronized contraction of the heart and in embryonic development.

ncbi gi num :

4504001

ncbi acc num :

NP_000156.1

ncbi gb acc num :

NM_000165.4

uniprot acc num :

P17302

ncbi pathways :

Arrhythmogenic Right Ventricular Cardiomyopathy Pathway (672454); Arrhythmogenic Right Ventricular Cardiomyopathy (ARVC) Pathway (117293); Arrhythmogenic Right Ventricular Cardiomyopathy (ARVC) Pathway (116129); Calcium Regulation In The Cardiac Cell Pathway (198906); Corticotropin-releasing Hormone Pathway (920957); EGFR1 Signaling Pathway (198782); Formation Of Annular Gap Junctions Pathway (105934); Gap Junction Pathway (83072); Gap Junction Pathway (483); Gap Junction Assembly Pathway (105928)

ncbi summary :

This gene is a member of the connexin gene family. The encoded protein is a component of gap junctions, which are composed of arrays of intercellular channels that provide a route for the diffusion of low molecular weight materials from cell to cell. The encoded protein is the major protein of gap junctions in the heart that are thought to have a crucial role in the synchronized contraction of the heart and in embryonic development. A related intronless pseudogene has been mapped to chromosome 5. Mutations in this gene have been associated with oculodentodigital dysplasia, autosomal recessive craniometaphyseal dysplasia and heart malformations. [provided by RefSeq, May 2014]

uniprot summary :

GJA1: an integral membrane protein of the connexin family, alpha-type (group II) subfamily. Hexamers of connexin-43 form connexons, which aggregate together to form gap junctions, through which materials of low MW diffuse from one cell to a neighboring cell. May play a critical role in the physiology of hearing by participating in the recycling of potassium to the cochlear endolymph. Protein type: Membrane protein, multi-pass; Membrane protein, integral; Channel, misc.; Motility/polarity/chemotaxis. Chromosomal Location of Human Ortholog: 6q22.31. Cellular Component: Golgi apparatus; endoplasmic reticulum membrane; focal adhesion; contractile fiber; integral to plasma membrane; lysosome; early endosome; intermediate filament; fascia adherens; cytosol; lipid raft; Golgi membrane; multivesicular body; connexon complex; mitochondrial outer membrane; apical plasma membrane; plasma membrane; gap junction; lateral plasma membrane. Molecular Function: signal transducer activity; protein binding; ion transmembrane transporter activity; beta-tubulin binding; SH3 domain binding; gap junction channel activity; receptor binding; PDZ domain binding. Biological Process: lens development in camera-type eye; response to peptide hormone stimulus; apoptosis; heart development; neuron migration; milk ejection; positive regulation of vasodilation; signal transduction; elevation of cytosolic calcium ion concentration; muscle contraction; cell-cell signaling; negative regulation of cardiac muscle cell proliferation; positive regulation of glomerular filtration; transport; response to glucose stimulus; positive regulation of striated muscle development; heart looping; adult heart development; ATP transport; chronic inflammatory response; positive regulation of I-kappaB kinase/NF-kappaB cascade; gap junction assembly; epithelial cell maturation; in utero embryonic development; regulation of bone remodeling; positive regulation of insulin secretion; skeletal muscle regeneration; regulation of calcium ion transport; vascular transport; protein oligomerization; osteoblast differentiation; positive regulation of osteoblast differentiation; negative regulation of endothelial cell proliferation; positive regulation of protein catabolic process; positive regulation of vasoconstriction; blood vessel morphogenesis; embryonic digit morphogenesis; regulation of bone mineralization; neurite morphogenesis; response to pH. Disease: Syndactyly, Type Iii; Craniometaphyseal Dysplasia, Autosomal Recessive; Palmoplantar Keratoderma And Congenital Alopecia 1; Atrioventricular Septal Defect 3; Oculodentodigital Dysplasia; Erythrokeratodermia Variabilis Et Progressiva; Oculodentodigital Dysplasia, Autosomal Recessive; Hypoplastic Left Heart Syndrome 1

size1 :

0.05 mL

price1 :

530 USD

more info or order :

MyBioSource product webpage