Rabbit Polyclonal to Human CD47 | MyBioSource MBS248758 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Rabbit Polyclonal to Human CD47

catalog :

MBS248758

quantity :

0.05 mg

price :

530 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human

application :

western blot, ELISA, immunohistochemistry, enzyme immunoassay

more info or order :

MyBioSource product webpage

image

image 1 :

Human Prostate: Formalin-Fixed, Paraffin-Embedded (FFPE)

image 2 :

product information

catalog number :

MBS248758

products type :

Antibody

products full name :

Rabbit Polyclonal to Human CD47

products short name :

[CD47]

products name syn :

[Anti-CD47 Antibody (aa23-140) IHC-plus; CD47; CD47 antigen; CD47 molecule; Integrin associated protein; Integrin-associated protein; Leukocyte surface antigen CD47; MER6; Protein MER6; Rh-related antigen; OA3; CD47 glycoprotein; IAP; Human CD47]

other names :

[leukocyte surface antigen CD47 isoform 1; Leukocyte surface antigen CD47; leukocyte surface antigen CD47; CD47 glycoprotein; Rh-related antigen; integrin associated protein; integrin-associated protein; integrin-associated signal transducer; antigenic surface determinant protein OA3; antigen identified by monoclonal antibody 1D8; CD47 antigen (Rh-related antigen, integrin-associated signal transducer); CD47 molecule; Antigenic surface determinant protein OA3; Integrin-associated protein; IAP; Protein MER6; CD_antigen: CD47]

products gene name :

[CD47]

other gene names :

[CD47; CD47; IAP; OA3; MER6; MER6; IAP]

uniprot entry name :

CD47_HUMAN

clonality :

Polyclonal

host :

Rabbit

reactivity :

Human

sequence length :

323

specificity :

Human CD47.

purity :

Affinity Purified

form :

PBS, pH 7.4, 0.02% sodium azide

concentration :

1 mg/ml

storage stability :

Store at -20 degree C for up to one year.

tested application :

Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)

app notes :

ELISA (1:20000 - 1:80000), IHC-P (2.5 - 5 ug/ml), WB (1:1000 - 1:2000). Optimal dilution to be determined by the researcher.

image1 heading :

Immunohistochemistry (IHC)

image2 heading :

Western Blot (WB)

other info1 :

Target: Human. Immunogen: CD47 antibody was raised against a recombinant human CD47 protein 23-140aa (BC010016). Immunogen Species: CD47 antibody was raised against Human

other info2 :

Antigen Type: Recombinant protein. Epitope: aa23-140. Disclaimer: Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application. Recommended Immunohistochemistry Protocol: The following protocol is a recommendation only, and MyBioSource, Inc. makes no guarantee of the results:. Tissue Preparation: . Formalin fixation and embedding in paraffin wax. Tissue Sectioning: . Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C. Deparaffinization: . Wash dry slides in 3 changes of xylene - 5 minutes each @ RT. Rehydration: . Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT. Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT. Wash slides in 1 change of 80% alcohol - 3 minutes @ RT. Rinse slides in gentle running distilled water - 5 minutes @ RT. Antigen retrieval: . Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes. Remove from heat and let stand at room temperature in buffer - 20 minutes. Rinse in 1X TBS with Tween (TBST) -1 minute @ RT. Immunostaining: . (Do not allow tissues to dry at any time during the staining procedure) . Apply a universal protein block - 20 minutes @ RT. Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT. Rinse slides in 1 X TBST - 1 minute @ RT. Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT. Rinse slides in 1X TBST -1 minute @ RT. Apply alkaline phosphatase streptavidin - 30 minutes @ RT. Rinse slides in 1X TBST -1 minute @ RT. Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT. Wash slides in distilled water - 1 minute @ RT. Dehydrate: . (This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB) . Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT. Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT. Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT. Wash slides in 3 changes of xylene - 1 minute each @ RT. Apply coverslip

products description :

CD47 is involved in the increase in intracellular calcium concentration that occurs upon cell adhesion to extracellular matrix. CD47 is also a receptor for the C-terminal cell binding domain of thrombospondin, and it may play a role in membrane transport and signal transduction. CD47 has broad tissue distribution, and is reduced in expression on Rh erythrocytes.

ncbi gi num :

4502673

ncbi acc num :

NP_001768.1

ncbi gb acc num :

NM_001777.3

uniprot acc num :

Q08722

ncbi mol weight :

33,845 Da

ncbi pathways :

Cell Surface Interactions At The Vascular Wall Pathway (106062); Cell-Cell Communication Pathway (477132); ECM-receptor Interaction Pathway (83068); ECM-receptor Interaction Pathway (479); Extracellular Matrix Organization Pathway (576262); Hemostasis Pathway (106028); Integrin Cell Surface Interactions Pathway (106110); Integrins In Angiogenesis Pathway (137999); Signal Regulatory Protein (SIRP) Family Interactions Pathway (187209); Spinal Cord Injury Pathway (739007)

ncbi summary :

This gene encodes a membrane protein, which is involved in the increase in intracellular calcium concentration that occurs upon cell adhesion to extracellular matrix. The encoded protein is also a receptor for the C-terminal cell binding domain of thrombospondin, and it may play a role in membrane transport and signal transduction. This gene has broad tissue distribution, and is reduced in expression on Rh erythrocytes. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jul 2010]

uniprot summary :

CD47: Has a role in both cell adhesion by acting as an adhesion receptor for THBS1 on platelets, and in the modulation of integrins. Plays an important role in memory formation and synaptic plasticity in the hippocampus. Receptor for SIRPA, binding to which prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells. Interaction with SIRPG mediates cell-cell adhesion, enhances superantigen-dependent T-cell-mediated proliferation and costimulates T-cell activation. May play a role in membrane transport and/or integrin dependent signal transduction. May prevent premature elimination of red blood cells. May be involved in membrane permeability changes induced following virus infection. 4 isoforms of the human protein are produced by alternative splicing. Protein type: Motility/polarity/chemotaxis; Membrane protein, multi-pass; Membrane protein, integral. Chromosomal Location of Human Ortholog: 3q13.1-q13.2. Cellular Component: integral to plasma membrane; plasma membrane. Molecular Function: protein binding. Biological Process: integrin-mediated signaling pathway; extracellular matrix organization and biogenesis; response to bacterium; positive regulation of phagocytosis; positive regulation of cell proliferation; positive regulation of cell-cell adhesion; positive regulation of T cell activation; opsonization; cell adhesion; blood coagulation; leukocyte migration; positive regulation of inflammatory response

size1 :

0.05 mg

price1 :

530 USD

more info or order :

MyBioSource product webpage