antibody

Rabbit Polyclonal to Human HTR2C / 5-HT2C Receptor

MBS248748

0.05 mL

530 USD

polyclonal

rabbit

nonconjugated

human, mouse, rat

western blot, immunohistochemistry, immunocytochemistry

Antibody

Rabbit Polyclonal to Human HTR2C / 5-HT2C Receptor

[HTR2C / 5-HT2C Receptor]

[Anti-HTR2C / 5-HT2C Receptor Antibody (aa400-450) IHC-plus; HTR2C; 5-HT-1C; 5-HT1c receptor; 5HT-1C; 5-HT2C; 5-HT2c receptor; 5HT2C Receptor; 5-HT-2C; 5-HT1C; 5-HTR2C; HTR1C; Serotonin 5-HT-1c receptor; Serotonin receptor 2C; Serotonin 1c receptor; Serotonin 5-HT-2C receptor; Serotonin 2c receptor; Human HTR2C; 5-HT2C Receptor]

[5-hydroxytryptamine receptor 2C isoform a; 5-hydroxytryptamine receptor 2C; 5-hydroxytryptamine receptor 2C; 5-HT1C; 5-HT-1C; 5-HT-2C; serotonin receptor 2C; serotonin 5-HT-2C receptor; 5-hydroxytryptamine receptor 1C; 5-hydroxytryptamine receptor-1c; 5-hydroxytryptamine (serotonin) receptor 2C, G protein-coupled; 5-hydroxytryptamine receptor 1C; 5-HT-1C; 5-HT1C; Serotonin receptor 2C]

[HTR2C]

[HTR2C; HTR2C; HTR1C; 5-HT2C; 5HTR2C; 5-HTR2C; HTR1C; 5-HT-2C; 5-HT2C; 5-HTR2C; 5-HT-1C; 5-HT1C]

5HT2C_HUMAN

Polyclonal

Rabbit

Human, Mouse, Rat

458

Human HTR2C / 5-HT2C

Immunoaffinity Purified

PBS, pH 7.2, 0.05% sodium azide

1 mg/ml

Store at 4 degree C short term. Aliquot and store at -20 degree C long term. Avoid freeze-thaw cycles.

Immunohistochemistry (IHC - Paraffin), Immunofluorescence (IF), Western Blot (WB)

IF (1:50 - 1:200), IHC-P (1:200), WB (1:500 - 1:1000). Optimal dilution to be determined by researcher

Immunohistochemistry (IHC)

Target: HTR2C/5-HT2C Receptor. Immunogen Species: HTR2C/5-HT2C receptor antibody was raised against Human. Antigen Type: synthetic peptide

Immunogen: HTR2C/5-HT2C receptor antibody was raised against synthetic peptide around Gln437 of human HTR2C/5-HT2C. Epitope: aa400-450. Disclaimer: Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application. Recommended Immunohistochemistry Protocol: The following protocol is a recommendation only, and MyBioSource, Inc. makes no guarantee of the results:. Tissue Preparation: . Formalin fixation and embedding in paraffin wax. Tissue Sectioning: . Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C. Deparaffinization: . Wash dry slides in 3 changes of xylene - 5 minutes each @ RT. Rehydration: . Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT. Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT. Wash slides in 1 change of 80% alcohol - 3 minutes @ RT. Rinse slides in gentle running distilled water - 5 minutes @ RT. Antigen retrieval: . Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes. Remove from heat and let stand at room temperature in buffer - 20 minutes. Rinse in 1X TBS with Tween (TBST) -1 minute @ RT. Immunostaining: . (Do not allow tissues to dry at any time during the staining procedure) . Apply a universal protein block - 20 minutes @ RT. Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT. Rinse slides in 1 X TBST - 1 minute @ RT. Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT. Rinse slides in 1X TBST -1 minute @ RT. Apply alkaline phosphatase streptavidin - 30 minutes @ RT. Rinse slides in 1X TBST -1 minute @ RT. Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT. Wash slides in distilled water - 1 minute @ RT. Dehydrate: . (This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB) . Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT. Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT. Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT. Wash slides in 3 changes of xylene - 1 minute each @ RT. Apply coverslip

Family: GPCR. Subfamily: Serotonin

5-HT2C, a Serotonin Receptor previously termed 5-HT1C, activates phospholipase C, resulting in a transient increase in intracellular calcium and activation of protein kinase C. Mutant mice lacking functional 5-HT2C receptor are overweight due to abnormal feeding behavior and are prone to epileptic seizures. Therefore, agonists of the 5-HT2C receptor such as m-chlorophenylpiperazine (mCPP) may be potential drugs for the treatment of obesity.

4504541

NP_000859.1

NM_000868.2

P28335

28,055 Da

Amine Ligand-binding Receptors Pathway (106363); Calcium Signaling Pathway (83050); Calcium Signaling Pathway (459); Class A/1 (Rhodopsin-like Receptors) Pathway (106357); Defective ACTH Causes Obesity And Pro-opiomelanocortinin Deficiency (POMCD) Pathway (1127664); Disease Pathway (530764); G Alpha (q) Signalling Events Pathway (106043); GPCR Downstream Signaling Pathway (119548); GPCR Ligand Binding Pathway (161020); GPCRs, Class A Rhodopsin-like Pathway (198886)

Serotonin (5-hydroxytryptamine, 5-HT), a neurotransmitter, elicits a wide array of physiological effects by binding to several receptor subtypes, including the 5-HT2 family of seven-transmembrane-spanning, G-protein-coupled receptors, which activate phospholipase C and D signaling pathways. This gene encodes the 2C subtype of serotonin receptor and its mRNA is subject to multiple RNA editing events, where genomically encoded adenosine residues are converted to inosines. RNA editing is predicted to alter amino acids within the second intracellular loop of the 5-HT2C receptor and generate receptor isoforms that differ in their ability to interact with G proteins and the activation of phospholipase C and D signaling cascades, thus modulating serotonergic neurotransmission in the central nervous system. Studies in humans have reported abnormalities in patterns of 5-HT2C editing in depressed suicide victims. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Feb 2012]

5-HT(2C): a G-protein coupled receptor. One of several different receptors for 5-hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. Mediates its action by activating a phosphatidylinositol-calcium second messenger system. Protein type: Receptor, GPCR; Membrane protein, integral; Membrane protein, multi-pass; GPCR, family 1. Chromosomal Location of Human Ortholog: Xq24. Cellular Component: integral to plasma membrane; plasma membrane. Molecular Function: serotonin binding; serotonin receptor activity; drug binding; 5-HT2 receptor activity. Biological Process: response to drug; cGMP biosynthetic process; elevation of cytosolic calcium ion concentration during G-protein signaling, coupled to IP3 second messenger (phospholipase C activating); behavioral fear response; regulation of corticotropin-releasing hormone secretion; serotonin receptor, phospholipase C activating pathway; locomotory behavior; positive regulation of phosphatidylinositol biosynthetic process; cellular calcium ion homeostasis; synaptic transmission; regulation of neurological process; release of sequestered calcium ion into cytosol; regulation of appetite; positive regulation of fat cell differentiation; feeding behavior; G-protein signaling, coupled to IP3 second messenger (phospholipase C activating); serotonin receptor signaling pathway

0.05 mL

530 USD