Rabbit Polyclonal to Mouse POSTN / Periostin | MyBioSource MBS248446 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Rabbit Polyclonal to Mouse POSTN / Periostin

catalog :

MBS248446

quantity :

0.05 mL

price :

530 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat, chicken

application :

western blot, immunohistochemistry

more info or order :

MyBioSource product webpage

image

image 1 :

Human Breast: Formalin-Fixed, Paraffin-Embedded (FFPE)

image 2 :

product information

catalog number :

MBS248446

products type :

Antibody

products full name :

Rabbit Polyclonal to Mouse POSTN / Periostin

products short name :

[POSTN / Periostin]

products name syn :

[Anti-POSTN / Periostin Antibody IHC-plus; POSTN; OSF2; PDLPOSTN; Periostin isoform thy6; Periostin isoform thy8; RP11-412K4.1; OSF-2; Osteoblast-specific factor 2; Periostin; Periostin isoform thy2; Periostin isoform thy4; PN; Mouse POSTN; Periostin]

other names :

[periostin isoform 1; Periostin; periostin; periodontal ligament-specific periostin; osteoblast specific factor 2 (fasciclin I-like); periostin, osteoblast specific factor; Osteoblast-specific factor 2; OSF-2]

products gene name :

[POSTN]

other gene names :

[POSTN; POSTN; PN; OSF2; OSF-2; PDLPOSTN; periostin; OSF2; PN; OSF-2]

uniprot entry name :

POSTN_HUMAN

clonality :

Polyclonal

host :

Rabbit

reactivity :

Chicken, Human, Mouse, Rat

sequence length :

836

specificity :

Based on the homology of the immunogen with other species, we expect that this antibody will recognize periostin in most, if not all, species of mammals, birds, amphibians, and fishes. Specific for three periostin bands around ~93 kDa in mouse lung extract. The immunogenic peptide is present in all known splice variants of periostin and therefore this antibody recognizes all known molecular forms of periostin.

purity :

Affinity Purified

form :

PBS. Contains no preservative.

storage stability :

Store at -20 degree C. Aliquot to avoid freeze/thaw cycles. Store undiluted.

tested application :

Immunohistochemistry (IHC - Paraffin), Western Blot (WB)

app notes :

IHC-P (1:100), WB (1:1000). (Optimal dilutions to be determined by the researcher). Usage: In addition to western blot, the antibody also works well for immunohistochemistry on paraformaldehyde-fixed sections with a simple antigen-retrieval protocol (incubate slides for 20 minutes at 90 degree C in 10 mM sodium citrate (pH 6.0)/ 0.1 % Tween-20). Disclaimer Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application.!! !!Recommended Immunohistochemistry Protocol The following protocol is a recommendation only, and MyBioSource, Inc. makes no guarantee of the results:. Tissue Preparation: . Formalin fixation and embedding in paraffin wax. Tissue Sectioning: . Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C. Deparaffinization: . Wash dry slides in 3 changes of xylene - 5 minutes each @ RT. Rehydration: . Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT. Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT. Wash slides in 1 change of 80% alcohol - 3 minutes @ RT. Rinse slides in gentle running distilled water - 5 minutes @ RT. Antigen retrieval: . Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes. Remove from heat and let stand at room temperature in buffer - 20 minutes. Rinse in 1X TBS with Tween (TBST) -1 minute @ RT. Immunostaining: . (Do not allow tissues to dry at any time during the staining procedure) . Apply a universal protein block - 20 minutes @ RT. Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT. Rinse slides in 1 X TBST - 1 minute @ RT. Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT. Rinse slides in 1X TBST -1 minute @ RT. Apply alkaline phosphatase streptavidin - 30 minutes @ RT. Rinse slides in 1X TBST -1 minute @ RT. Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT. Wash slides in distilled water - 1 minute @ RT. Dehydrate: . (This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB) . Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT. Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT. Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT. Wash slides in 3 changes of xylene - 1 minute each @ RT. Apply coverslip

image1 heading :

Immunohistochemistry (IHC)

image2 heading :

Western Blot (WB)

other info1 :

Target: POSTN/Periostin. Immunogen Description: Antibody was raised against peptide from fasciclin domain 1 of mouse periostin. Immunogen Species: Antibody was raised against Mouse.

ncbi gi num :

209862907

ncbi acc num :

NP_006466.2

ncbi gb acc num :

NM_006475.2

uniprot acc num :

Q15063

ncbi mol weight :

80,357 Da

ncbi summary :

This gene encodes a secreted extracellular matrix protein that functions in tissue development and regeneration, including wound healing, and ventricular remodeling following myocardial infarction. The encoded protein binds to integrins to support adhesion and migration of epithelial cells. This protein plays a role in cancer stem cell maintenance and metastasis. Mice lacking this gene exhibit cardiac valve disease, and skeletal and dental defects. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Sep 2015]

uniprot summary :

POSTN: Binds to heparin. Induces cell attachment and spreading and plays a role in cell adhesion. May play a role in extracellular matrix mineralization. 4 isoforms of the human protein are produced by alternative splicing. Protein type: Secreted; Cell adhesion; Secreted, signal peptide; Motility/polarity/chemotaxis. Chromosomal Location of Human Ortholog: 13q13.3. Cellular Component: extracellular matrix; extracellular space; proteinaceous extracellular matrix; trans-Golgi network. Molecular Function: heparin binding; protein binding; cell adhesion molecule binding. Biological Process: tissue development; extracellular matrix organization and biogenesis; regulation of Notch signaling pathway; cell adhesion; skeletal development

size1 :

0.05 mL

price1 :

530 USD

more info or order :

MyBioSource product webpage