Rabbit Polyclonal (IgG) to Human OLFML2B | MyBioSource MBS248408 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Rabbit Polyclonal (IgG) to Human OLFML2B

catalog :

MBS248408

quantity :

0.05 mL

price :

530 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse

application :

western blot, ELISA, immunohistochemistry, enzyme immunoassay

more info or order :

MyBioSource product webpage

image

image 1 :

Anti-OLFML2B antibody IHC staining of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

image 2 :

Western blot of extracts from HUVEC cells, using OLFML2B Antibody. The lane on the right is treated with the synthesized peptide.

product information

catalog number :

MBS248408

products type :

Antibody

products full name :

Rabbit Polyclonal (IgG) to Human OLFML2B

products short name :

[OLFML2B]

products name syn :

[Anti-OLFML2B Antibody (aa171-220) IHC-plus; OLFML2B; DKFZP586L151; Photomedin-2; RP11-227F8.1; Olfactomedin-like 2B; Olfactomedin-like protein 2B; Human OLFML2B]

other names :

[olfactomedin-like protein 2B isoform 2; Olfactomedin-like protein 2B; olfactomedin-like protein 2B; photomedin-2; olfactomedin-like 2B; Photomedin-2]

products gene name :

[OLFML2B]

other gene names :

[OLFML2B; OLFML2B]

uniprot entry name :

OLM2B_HUMAN

clonality :

Polyclonal

isotype :

IgG

host :

Rabbit

reactivity :

Human, Mouse

sequence length :

750

specificity :

OLFML2B Antibody detects endogenous levels of total OLFML2B protein.

purity :

Immunoaffinity Purified

form :

PBS (without Mg2+, Ca2+), pH 7.4, 150 mM NaCl, 0.02% sodium azide, 50% Glycerol

concentration :

1 mg/ml

storage stability :

Store at -20 degree C for up to one year.

tested application :

Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)

app notes :

IHC-P (1:50). WB (1:500 - 1:1000). Peptide Enzyme-Linked Immunosorbent Assay (1:10000). optimal dilution to be determined by the researcher. Usage Summary: Western Blot: Predicted MW 83 kDa

image1 heading :

Immunohistochemistry (IHC)

image2 heading :

Western Blot (WB)

other info1 :

Family/Subfamily: GPCR/Orapha-B. Immunogen Species: OLFML2B antibody was raised against Human

other info2 :

Immunogen: OLFML2B antibody was raised against synthetic peptide from human OLFML2B (aa171-220). Epitope: aa171-220. Disclaimer: Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application. Recommended Immunohistochemistry Protocol: The following protocol is a recommendation only, and MyBioSource, Inc. makes no guarantee of the results:. Tissue Preparation: . Formalin fixation and embedding in paraffin wax. Tissue Sectioning: . Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C. Deparaffinization: . Wash dry slides in 3 changes of xylene - 5 minutes each @ RT. Rehydration: . Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT. Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT. Wash slides in 1 change of 80% alcohol - 3 minutes @ RT. Rinse slides in gentle running distilled water - 5 minutes @ RT. Antigen retrieval: . Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes. Remove from heat and let stand at room temperature in buffer - 20 minutes. Rinse in 1X TBS with Tween (TBST) -1 minute @ RT. Immunostaining: . (Do not allow tissues to dry at any time during the staining procedure) . Apply a universal protein block - 20 minutes @ RT. Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT. Rinse slides in 1 X TBST - 1 minute @ RT. Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT. Rinse slides in 1X TBST -1 minute @ RT. Apply alkaline phosphatase streptavidin - 30 minutes @ RT. Rinse slides in 1X TBST -1 minute @ RT. Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT. Wash slides in distilled water - 1 minute @ RT. Dehydrate: . (This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB) . Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT. Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT. Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT. Wash slides in 3 changes of xylene - 1 minute each @ RT. Apply coverslip

products categories :

Family: GPCR. Subfamily: Orphan-B

products description :

G Protein-Coupled Receptor Ls195570 is an Orphan-B GPCR with an unknown ligand.

ncbi gi num :

46195719

ncbi acc num :

NP_056256.1

ncbi gb acc num :

NM_015441.2

uniprot acc num :

Q68BL8

uniprot summary :

OLFML2B: 2 isoforms of the human protein are produced by alternative splicing. Protein type: Secreted, signal peptide; Secreted; Extracellular matrix. Chromosomal Location of Human Ortholog: 1q23.3. Cellular Component: extracellular region

size1 :

0.05 mL

price1 :

530 USD

more info or order :

MyBioSource product webpage