Rabbit Polyclonal (IgG) to Human CHRM4 / M4 | MyBioSource MBS248068 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Rabbit Polyclonal (IgG) to Human CHRM4 / M4

catalog :

MBS248068

quantity :

0.05 mL

price :

530 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

ELISA, immunohistochemistry, immunocytochemistry, enzyme immunoassay

more info or order :

MyBioSource product webpage

image

image 1 :

Anti-CHRM4 / M4 antibody IHC staining of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

image 2 :

Immunofluorescence of LOVO cells, using CHRM4 Antibody. The picture on the right is treated with the synthesized peptide.

product information

catalog number :

MBS248068

products type :

Antibody

products full name :

Rabbit Polyclonal (IgG) to Human CHRM4 / M4

products short name :

[CHRM4 / M4]

products name syn :

[CHRM4 Antibody, M4 muscarinic receptor Antibody, M4R Antibody, Muscarinic m4 receptor Antibody, HM4 Antibody, Muscarinic receptor m4 Antibody]

other names :

[muscarinic acetylcholine receptor M4; Muscarinic acetylcholine receptor M4; muscarinic acetylcholine receptor M4; acetylcholine receptor, muscarinic 4; cholinergic receptor, muscarinic 4]

products gene name :

[CHRM4]

other gene names :

[CHRM4; CHRM4; HM4; M4R]

uniprot entry name :

ACM4_HUMAN

clonality :

Polyclonal

isotype :

IgG

host :

Rabbit

reactivity :

Human, Mouse, Rat

sequence length :

479

specificity :

CHRM4 Antibody detects endogenous levels of total CHRM4 protein.

purity :

Immunoaffinity Purified

form :

PBS (without Mg2+, Ca2+), pH 7.4, 150 mM sodium chloride, 0.02% sodium azide, 50% glycerol

storage stability :

Store at -20 degree C for up to one year.

tested application :

Immunohistochemistry (IHC - Paraffin), Immunofluorescence (IF), ELISA (EIA)

app notes :

ELISA (1:10000). IF (1:100 - 1:500). IHC-P (1:50). Optimal dilution to be determined by the researcher)

image1 heading :

Immunohistochemistry (IHC)

image2 heading :

Immunofluorescence (IF)

other info1 :

Target: CHRM4/M4. Immunogen: Antibody was raised against synthetic peptide from human CHRM4 (aa236-285).

other info2 :

Immunogen species: CHRM4 / M4 antibody was raised against human. Epitope: aa236-285. Antigen Type: Synethetic peptide. Disclaimer: Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application. Recommended Immunohistochemistry Protocol: The following protocol is a recommendation only, and MyBioSource, Inc. makes no guarantee of the results:. Tissue Preparation: . Formalin fixation and embedding in paraffin wax. Tissue Sectioning: . Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C. Deparaffinization: . Wash dry slides in 3 changes of xylene - 5 minutes each @ RT. Rehydration: . Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT. Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT. Wash slides in 1 change of 80% alcohol - 3 minutes @ RT. Rinse slides in gentle running distilled water - 5 minutes @ RT. Antigen retrieval: . Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes. Remove from heat and let stand at room temperature in buffer - 20 minutes. Rinse in 1X TBS with Tween (TBST) -1 minute @ RT. Immunostaining: . (Do not allow tissues to dry at any time during the staining procedure) . Apply a universal protein block - 20 minutes @ RT. Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT. Rinse slides in 1 X TBST - 1 minute @ RT. Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT. Rinse slides in 1X TBST -1 minute @ RT. Apply alkaline phosphatase streptavidin - 30 minutes @ RT. Rinse slides in 1X TBST -1 minute @ RT. Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT. Wash slides in distilled water - 1 minute @ RT. Dehydrate: . (This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB) . Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT. Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT. Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT. Wash slides in 3 changes of xylene - 1 minute each @ RT. Apply coverslip

products categories :

Family: GPCR. Subfamily: Acetylcholine

products description :

Muscarinic acetylcholine receptor M4 is an Acetylcholine Receptor. Unlike muscarinic receptor M2, the M4 receptor is not involved in smooth muscle contraction. The clinical relevance of this receptor is unknown. However, mouse studies have linked it to adenylyl cyclase inhibition.

ncbi gi num :

52426748

ncbi acc num :

NP_000732.2

ncbi gb acc num :

NM_000741.2

uniprot acc num :

P08173

ncbi pathways :

Amine Ligand-binding Receptors Pathway (106363); Calcium Regulation In The Cardiac Cell Pathway (198906); Cholinergic Synapse Pathway (217716); Class A/1 (Rhodopsin-like Receptors) Pathway (106357); Defective ACTH Causes Obesity And Pro-opiomelanocortinin Deficiency (POMCD) Pathway (1127664); Disease Pathway (530764); G Alpha (i) Signalling Events Pathway (119550); GPCR Downstream Signaling Pathway (119548); GPCR Ligand Binding Pathway (161020); GPCRs, Class A Rhodopsin-like Pathway (198886)

ncbi summary :

The muscarinic cholinergic receptors belong to a larger family of G protein-coupled receptors. The functional diversity of these receptors is defined by the binding of acetylcholine and includes cellular responses such as adenylate cyclase inhibition, phosphoinositide degeneration, and potassium channel mediation. Muscarinic receptors influence many effects of acetylcholine in the central and peripheral nervous system. The clinical implications of this receptor are unknown; however, mouse studies link its function to adenylyl cyclase inhibition. [provided by RefSeq, Jul 2008]

uniprot summary :

mAChR M4: The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is inhibition of adenylate cyclase. Belongs to the G-protein coupled receptor 1 family. Muscarinic acetylcholine receptor subfamily. CHRM4 sub-subfamily. Protein type: Membrane protein, multi-pass; Receptor, GPCR; Membrane protein, integral; GPCR, family 1. Chromosomal Location of Human Ortholog: 11p12-p11.2. Cellular Component: postsynaptic membrane; integral to plasma membrane; plasma membrane; cell junction. Molecular Function: G-protein coupled acetylcholine receptor activity. Biological Process: regulation of locomotion; cell proliferation; acetylcholine receptor signaling, muscarinic pathway; cell surface receptor linked signal transduction; muscarinic acetylcholine receptor, adenylate cyclase inhibiting pathway; signal transduction

size1 :

0.05 mL

price1 :

530 USD

more info or order :

MyBioSource product webpage