Rabbit Polyclonal (IgG) to Human SLC16A2 / MCT8 | MyBioSource MBS248052 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Rabbit Polyclonal (IgG) to Human SLC16A2 / MCT8

catalog :

MBS248052

quantity :

0.05 mL

price :

530 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry

more info or order :

MyBioSource product webpage

image

image 1 :

Anti-SLC16A2 / MCT8 antibody IHC staining of human adrenal. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

image 2 :

Western blot of the lysates from HT-29 cells using SLC16A2 antibody.

product information

catalog number :

MBS248052

products type :

Antibody

products full name :

Rabbit Polyclonal (IgG) to Human SLC16A2 / MCT8

products short name :

[SLC16A2 / MCT8]

products name syn :

[Anti-SLC16A2 / MCT8 Antibody (aa93-142) IHC-plus; SLC16A2; Allan-Herndon-Dudley syndrome; AHDS; DXS128E; DXS128; MCT8; Monocarboxylate transporter 7; Monocarboxylate transporter 8; MCT 7; MCT 8; MRX22; XPCT; MCT7; Human SLC16A2; MCT8]

other names :

[monocarboxylate transporter 8; Monocarboxylate transporter 8; monocarboxylate transporter 8; monocarboxylate transporter 7; X-linked PEST-containing transporter; solute carrier family 16, member 2 (thyroid hormone transporter); Monocarboxylate transporter 7; MCT 7; Solute carrier family 16 member 2; X-linked PEST-containing transporter]

products gene name :

[SLC16A2]

products gene name syn :

[MCT8]

other gene names :

[SLC16A2; SLC16A2; AHDS; MCT7; MCT8; XPCT; MCT 7; MCT 8; MRX22; DXS128; DXS128E; MCT8; XPCT; MCT 8; MCT 7]

uniprot entry name :

MOT8_HUMAN

clonality :

Polyclonal

isotype :

IgG

host :

Rabbit

reactivity :

Human, Mouse, Rat

specificity :

SLC16A2/MCT8 antibody detects endogenous levels of SLC16A2.

purity :

Immunoaffinity purified

form :

PBS, pH 7.4, 150mM NaCl, 0.02% Sodium Azide, 50% Glycerol

concentration :

1mg/ml

storage stability :

Store at -20°C.

tested application :

Immunohistochemistry - Paraffin (IHC), Western Blot (WB), Peptide ELISA

app notes :

IHC - Paraffin (1:200). Western Blot (1:500-1:100). Peptide ELISA (1:10000). (Optimal dilution to be determined by the researcher.)

image1 heading :

Immunohistochemistry (IHC)

image2 heading :

Western Blot (WB)

other info1 :

Immunogen Species: SLC16A2/MCT8 antibody was raised against Human. Immunogen: SLC16A2/MCT8 antibody was raised against synthetic peptide from human SLC16A2. (AA Range: 93-142). Epitope: aa93-142

other info2 :

Disclaimer: Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application. Recommended Immunohistochemistry Protocol: The following protocol is a recommendation only, and MyBioSource, Inc. makes no guarantee of the results:. Tissue Preparation: . Formalin fixation and embedding in paraffin wax. Tissue Sectioning: . Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C. Deparaffinization: . Wash dry slides in 3 changes of xylene - 5 minutes each @ RT. Rehydration: . Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT. Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT. Wash slides in 1 change of 80% alcohol - 3 minutes @ RT. Rinse slides in gentle running distilled water - 5 minutes @ RT. Antigen retrieval: . Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes. Remove from heat and let stand at room temperature in buffer - 20 minutes. Rinse in 1X TBS with Tween (TBST) -1 minute @ RT. Immunostaining: . (Do not allow tissues to dry at any time during the staining procedure) . Apply a universal protein block - 20 minutes @ RT. Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT. Rinse slides in 1 X TBST - 1 minute @ RT. Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT. Rinse slides in 1X TBST -1 minute @ RT. Apply alkaline phosphatase streptavidin - 30 minutes @ RT. Rinse slides in 1X TBST -1 minute @ RT. Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT. Wash slides in distilled water - 1 minute @ RT. Dehydrate: . (This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB) . Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT. Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT. Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT. Wash slides in 3 changes of xylene - 1 minute each @ RT. Apply coverslip

products categories :

Family: Transporter. Subfamily: Monocarboxylic acid transporter

ncbi gi num :

5730045

ncbi acc num :

NP_006508.1

ncbi gb acc num :

NM_006517.3

uniprot acc num :

P36021

ncbi mol weight :

Western Blot: Predicted MW 59 kDa

ncbi pathways :

Thyroid Hormone Signaling Pathway (946598)

ncbi summary :

This gene encodes an integral membrane protein that functions as a transporter of thyroid hormone. The encoded protein facilitates the cellular importation of thyroxine (T4), triiodothyronine (T3), reverse triiodothyronine (rT3) and diidothyronine (T2). This gene is expressed in many tissues and likely plays an important role in the development of the central nervous system. Loss of function mutations in this gene are associated with psychomotor retardation in males while females exhibit no neurological defects and more moderate thyroid-deficient phenotypes. This gene is subject to X-chromosome inactivation. Mutations in this gene are the cause of Allan-Herndon-Dudley syndrome. [provided by RefSeq, Mar 2012]

uniprot summary :

SLC16A2: Very active and specific thyroid hormone transporter. Stimulates cellular uptake of thyroxine (T4), triiodothyronine (T3), reverse triiodothyronine (rT3) and diidothyronine. Does not transport Leu, Phe, Trp or Tyr. Defects in SLC16A2 are the cause of monocarboxylate transporter 8 deficiency (MCT8 deficiency); also known as Allan-Herndon-Dudley syndrome (AHDS). MCT8 deficiency consists of a severe form of X-linked psychomotor retardation combined with abnormal thyroid hormone (TH) levels. Thyroid hormone deficiency can be caused by defects of hormone synthesis and action, but it has also been linked to a defect in cellular hormone transport. Affected patients are males with abnormal relative concentrations of three circulating iodothyronines, as well as severe neurological abnormalities, including global developmental delay, central hypotonia, spastic quadriplegia, dystonic movements, rotary nystagmus, and impaired gaze and hearing. Heterozygous females had a milder thyroid phenotype and no neurological defects. Belongs to the major facilitator superfamily. Monocarboxylate porter (TC 2.A.1.13) family. Protein type: Transporter; Transporter, SLC family; Membrane protein, multi-pass; Membrane protein, integral. Chromosomal Location of Human Ortholog: Xq13.2. Cellular Component: membrane; integral to plasma membrane; integral to membrane. Molecular Function: thyroid hormone transmembrane transporter activity; monocarboxylic acid transmembrane transporter activity; transporter activity; symporter activity. Biological Process: monocarboxylic acid transport; transport. Disease: Allan-herndon-dudley Syndrome

size1 :

0.05 mL

price1 :

530 USD

more info or order :

MyBioSource product webpage