Rabbit Polyclonal to Human SYVN1 / HRD1 | MyBioSource MBS247950 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Rabbit Polyclonal to Human SYVN1 / HRD1

catalog :

MBS247950

quantity :

0.05 mg

price :

530 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat, dog, cow, zebrafish

application :

immunohistochemistry, immunohistochemistry - paraffin section

more info or order :

MyBioSource product webpage

image

image 1 :

Anti-SYVN1 / HRD1 antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

product information

catalog number :

MBS247950

products type :

Antibody

products full name :

Rabbit Polyclonal to Human SYVN1 / HRD1

products short name :

[SYVN1 / HRD1]

products name syn :

[Anti-SYVN1 / HRD1 Antibody (Internal) IHC-plus; SYVN1; KIAA1810; Synoviolin; Synovial apoptosis inhibitor 1; DER3; HRD1; Human SYVN1; HRD1]

other names :

[E3 ubiquitin-protein ligase synoviolin isoform a; E3 ubiquitin-protein ligase synoviolin; E3 ubiquitin-protein ligase synoviolin; HMG-coA reductase degradation 1 homolog; synovial apoptosis inhibitor 1, synoviolin; Synovial apoptosis inhibitor 1]

products gene name :

[SYVN1]

products gene name syn :

[HRD1]

other gene names :

[SYVN1; SYVN1; DER3; HRD1; HRD1; KIAA1810]

uniprot entry name :

SYVN1_HUMAN

clonality :

Polyclonal

host :

Rabbit

reactivity :

Bat, Gibbon, Bovine, Dog, Xenopus, Gorilla, Hamster, Horse, Human, Monkey, Mouse, Rabbit, Rat, Zebrafish

sequence length :

616

specificity :

Human SYVN1 / HRD1. BLAST analysis of the peptide immunogen showed no homology with other human proteins.

purity :

Immunoaffinity Purified

form :

PBS, 0.1% sodium azide.

concentration :

1 mg/ml

storage stability :

Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to one month. Avoid freeze-thaw cycles.

tested application :

Immunohistochemistry (IHC - Paraffin)

app notes :

IHC-P (5 - 10 ug/ml)

image1 heading :

Immunohistochemistry (IHC)

other info1 :

Target Species: Human. Immunogen Description: Synthetic 18 amino acid peptide from internal region of human SYVN1 / HRD1. Antigen Type: Synthetic peptide. Immunogen: SYVN1 / HRD1 antibody was raised against synthetic 18 amino acid peptide from internal region of human SYVN1 / HRD1. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Bovine, Dog, Bat, Horse, Rabbit, Opossum, Xenopus, Stickleback, Pufferfish, Zebrafish (100%). Antigen Modification: Internal

other info2 :

. Disclaimer: Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application. Recommended Immunohistochemistry Protocol: The following protocol is a recommendation only, and MyBioSource, Inc. makes no guarantee of the results:. Tissue Preparation: . Formalin fixation and embedding in paraffin wax. Tissue Sectioning: . Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C. Deparaffinization: . Wash dry slides in 3 changes of xylene - 5 minutes each @ RT. Rehydration: . Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT. Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT. Wash slides in 1 change of 80% alcohol - 3 minutes @ RT. Rinse slides in gentle running distilled water - 5 minutes @ RT. Antigen retrieval: . Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes. Remove from heat and let stand at room temperature in buffer - 20 minutes. Rinse in 1X TBS with Tween (TBST) -1 minute @ RT. Immunostaining: . (Do not allow tissues to dry at any time during the staining procedure) . Apply a universal protein block - 20 minutes @ RT. Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT. Rinse slides in 1 X TBST - 1 minute @ RT. Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT. Rinse slides in 1X TBST -1 minute @ RT. Apply alkaline phosphatase streptavidin - 30 minutes @ RT. Rinse slides in 1X TBST -1 minute @ RT. Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT. Wash slides in distilled water - 1 minute @ RT. Dehydrate: . (This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB) . Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT. Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT. Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT. Wash slides in 3 changes of xylene - 1 minute each @ RT. Apply coverslip

ncbi gi num :

27436925

ncbi acc num :

NP_115807.1

ncbi gb acc num :

NM_032431.2

uniprot acc num :

Q86TM6

ncbi pathways :

Disease Pathway (530764); HRD1/SEL1 ERAD Complex Pathway (413468); HRD1/SEL1 ERAD Complex Pathway (468401); Hedgehog Ligand Biogenesis Pathway (1127536); Hh Ligand Biogenesis Disease Pathway (1127636); IRE1alpha Activates Chaperones Pathway (105906); Metabolism Of Proteins Pathway (106230); Processing-defective Hh Variants Abrogate Ligand Secretion Pathway (1127635); Protein Processing In Endoplasmic Reticulum Pathway (167325); Protein Processing In Endoplasmic Reticulum Pathway (167190)

ncbi summary :

This gene encodes a protein involved in endoplasmic reticulum (ER)-associated degradation. The encoded protein removes unfolded proteins, accumulated during ER stress, by retrograde transport to the cytosol from the ER. This protein also uses the ubiquitin-proteasome system for additional degradation of unfolded proteins. Sequence analysis identified two transcript variants that encode different isoforms. [provided by RefSeq, May 2011]

uniprot summary :

SYVN1: Acts as an E3 ubiquitin-protein ligase which accepts ubiquitin specifically from endoplasmic reticulum-associated UBC7 E2 ligase and transfers it to substrates, promoting their degradation. Component of the endoplasmic reticulum quality control (ERQC) system also called ER-associated degradation (ERAD) involved in ubiquitin-dependent degradation of misfolded endoplasmic reticulum proteins. Also promotes the degradation of normal but naturally short-lived proteins such as SGK. Protects cells from ER stress-induced apoptosis. Protects neurons from apoptosis induced by polyglutamine-expanded huntingtin (HTT) or unfolded GPR37 by promoting their degradation. Sequesters p53/TP53 in the cytoplasm and promotes its degradation, thereby negatively regulating its biological function in transcription, cell cycle regulation and apoptosis. Belongs to the HRD1 family. 3 isoforms of the human protein are produced by alternative splicing. Protein type: Membrane protein, integral; Ubiquitin conjugating system; EC 6.3.2.19; Ubiquitin ligase; Endoplasmic reticulum; Membrane protein, multi-pass; Ligase; EC 6.3.2.-. Chromosomal Location of Human Ortholog: 11q13. Cellular Component: nucleoplasm; endoplasmic reticulum membrane; membrane; endoplasmic reticulum; integral to membrane. Molecular Function: protein binding; acid-amino acid ligase activity; zinc ion binding. Biological Process: ER-associated protein catabolic process; cellular protein metabolic process; unfolded protein response, activation of signaling protein activity; in utero embryonic development; unfolded protein response; protein ubiquitination; protein amino acid N-linked glycosylation via asparagine

size1 :

0.05 mg

price1 :

530 USD

more info or order :

MyBioSource product webpage