Rabbit Polyclonal to Human C3AR / C3a Receptor | MyBioSource MBS247882 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Rabbit Polyclonal to Human C3AR / C3a Receptor

catalog :

MBS247882

quantity :

0.05 mg

price :

530 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human

application :

immunohistochemistry, immunohistochemistry - paraffin section

more info or order :

MyBioSource product webpage

image

image 1 :

Anti-C3AR / C3a Receptor antibody IHC of human lung, respiratory epithelium. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 17.3 ug/ml.

image 2 :

Anti-C3AR / C3a Receptor antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 17.3 ug/ml.

product information

catalog number :

MBS247882

products type :

Antibody

products full name :

Rabbit Polyclonal to Human C3AR / C3a Receptor

products short name :

[C3AR / C3a Receptor]

products name syn :

[Anti-C3AR / C3a Receptor Antibody (Extracellular Domain) IHC-plus; C3AR1; AZ3B; C3AR; C3R1; C3a receptor; C3a-R; HNFAG09; Anaphylatoxin c3a receptor; C3a anaphylatoxin receptor; Human C3AR; C3a Receptor]

other names :

[C3a anaphylatoxin chemotactic receptor; C3a anaphylatoxin chemotactic receptor; C3a anaphylatoxin chemotactic receptor; C3a-R; complement component 3 receptor 1; complement component 3a receptor 1]

products gene name :

[C3AR1]

other gene names :

[C3AR1; C3AR1; AZ3B; C3AR; HNFAG09; AZ3B; C3R1; HNFAG09; C3AR; C3a-R]

uniprot entry name :

C3AR_HUMAN

clonality :

Polyclonal

host :

Rabbit

reactivity :

Human. Predicted Reactivity: Monkey

sequence length :

482

specificity :

Human C3a Receptor. BLAST analysis of the peptide immunogen showed no homology with other human proteins, except ADRA1A (40%).

purity :

Immunoaffinity Purified

form :

PBS, 0.1% sodium azide.

concentration :

1 mg/ml

storage stability :

Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.

tested application :

Immunohistochemistry (IHC - Paraffin)

app notes :

IHC-P (17.3 ug/ml). Optimal dilution to be determined by the researcher.

image1 heading :

Immunohistochemistry (IHC)

image2 heading :

Immunohistochemistry (IHC)

other info1 :

Target Species: C3AR/C3a Receptor. Family/Subfamily: GPCR/Anaphylatoxin. Immunogen Species: C3AR/C3a Receptor antibody was raised againt Human. Antigen Type: Synthetic peptide. Immunogen: C3AR / C3a Receptor antibody was raised against synthetic 20 amino acid peptide from 2nd extracellular domain of human C3a Receptor. Percent identity with other species by BLAST analysis: Human (100%); Gorilla, Marmoset (95%); Orangutan, Gibbon (90%). Epitope Domain: Extracellular Domain

other info2 :

Disclaimer: Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application. Recommended Immunohistochemistry Protocol: The following protocol is a recommendation only, and MyBioSource, Inc. makes no guarantee of the results:. Tissue Preparation: . Formalin fixation and embedding in paraffin wax. Tissue Sectioning: . Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C. Deparaffinization: . Wash dry slides in 3 changes of xylene - 5 minutes each @ RT. Rehydration: . Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT. Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT. Wash slides in 1 change of 80% alcohol - 3 minutes @ RT. Rinse slides in gentle running distilled water - 5 minutes @ RT. Antigen retrieval: . Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes. Remove from heat and let stand at room temperature in buffer - 20 minutes. Rinse in 1X TBS with Tween (TBST) -1 minute @ RT. Immunostaining: . (Do not allow tissues to dry at any time during the staining procedure) . Apply a universal protein block - 20 minutes @ RT. Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT. Rinse slides in 1 X TBST - 1 minute @ RT. Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT. Rinse slides in 1X TBST -1 minute @ RT. Apply alkaline phosphatase streptavidin - 30 minutes @ RT. Rinse slides in 1X TBST -1 minute @ RT. Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT. Wash slides in distilled water - 1 minute @ RT. Dehydrate: . (This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB) . Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT. Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT. Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT. Wash slides in 3 changes of xylene - 1 minute each @ RT. Apply coverslip. Note: During shipment,small volumes of product will ocassionally become entrapped in the seal of the product vial. We recommend briefly centrifuging the vial to dislodge any liquid in the container's cap prior to opening. Warning: This reagent may contain sodium azide. The chemical, physical, and toxicologial properties of this material have not been thoughly investigated.Standard Laboratory Practices should be followed. Avoid skin and eye contact,inhalation, and ingestion. Sodium azide forms hydrazoic acid under acidic conditions and may react with lead or copper plubing to form highly explosive metal azides. On disposal, flush with large volumes of water to prevent accumulation.

products categories :

Family: GPCR. Subfamily: Anaphylatoxin

products description :

The C3a Anaphylatoxin Receptor mediates the effects of the inflammatory mediator anaphylatoxin C3a, which is released during complement activation. This receptor stimulates chemotaxis, granule enzyme release, and superoxide anion production. It also regulates glial cell activation, may be involved in the pathogenesis of asthma, and may promote cell repair.

ncbi gi num :

4757888

ncbi acc num :

NP_004045.1

ncbi gb acc num :

NM_004054.2

uniprot acc num :

Q16581

ncbi pathways :

Class A/1 (Rhodopsin-like Receptors) Pathway (106357); Complement And Coagulation Cascades Pathway (198880); Complement And Coagulation Cascades Pathway (83073); Complement And Coagulation Cascades Pathway (484); Defective ACTH Causes Obesity And Pro-opiomelanocortinin Deficiency (POMCD) Pathway (1127664); Disease Pathway (530764); G Alpha (i) Signalling Events Pathway (119550); GPCR Downstream Signaling Pathway (119548); GPCR Ligand Binding Pathway (161020); GPCRs, Class A Rhodopsin-like Pathway (198886)

uniprot summary :

C3aR: Receptor for the chemotactic and inflammatory peptide anaphylatoxin C3a. This receptor stimulates chemotaxis, granule enzyme release and superoxide anion production. Belongs to the G-protein coupled receptor 1 family. Protein type: GPCR, family 1; Membrane protein, multi-pass; Receptor, GPCR; Membrane protein, integral. Chromosomal Location of Human Ortholog: 12p13.31. Cellular Component: integral to plasma membrane; plasma membrane. Molecular Function: G-protein coupled receptor activity; C3a anaphylatoxin receptor activity; complement component C3a receptor activity; phosphoinositide phospholipase C activity. Biological Process: G-protein coupled receptor protein signaling pathway; elevation of cytosolic calcium ion concentration; positive regulation of angiogenesis; metabolic process; blood circulation; complement receptor mediated signaling pathway; chemotaxis; inflammatory response

size1 :

0.05 mg

price1 :

530 USD

more info or order :

MyBioSource product webpage