Goat Polyclonal to Human DDX58 / RIG-1 / RIG-I | MyBioSource MBS247247 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Goat Polyclonal to Human DDX58 / RIG-1 / RIG-I

catalog :

MBS247247

quantity :

0.05 mg

price :

495 USD

clonality :

polyclonal

host :

goat

conjugate :

nonconjugated

reactivity :

human

application :

immunohistochemistry, immunohistochemistry - paraffin section

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS247247

products type :

Antibody

products full name :

Goat Polyclonal to Human DDX58 / RIG-1 / RIG-I

products short name :

DDX58 / RIG-1 / RIG-I

products name syn :

Anti-DDX58 / RIG-1 / RIG-I Antibody IHC-plus; DDX58; RIG-1; RIG-I; RLR-1; Retinoic acid inducible gene I; RIG-I-like receptor 1; RIGI; DEAD box protein 58; RNA helicase RIG-I; Human DDX58; RIG-1; RIG-I

other names :

probable ATP-dependent RNA helicase DDX58; Probable ATP-dependent RNA helicase DDX58; probable ATP-dependent RNA helicase DDX58; RNA helicase RIG-I; DEAD box protein 58; retinoic acid-inducible gene 1 protein; retinoic acid-inducible gene I protein; DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide; DEAD (Asp-Glu-Ala-Asp) box polypeptide 58; DEAD box protein 58; RIG-I-like receptor 1; RLR-1; Retinoic acid-inducible gene 1 protein; RIG-1; Retinoic acid-inducible gene I protein; RIG-I

products gene name :

DDX58

products gene name syn :

RIG-1; RIG-I

other gene names :

DDX58; DDX58; RIGI; RIG-I; RLR-1; RLR-1; RIG-1; RIG-I

uniprot entry name :

DDX58_HUMAN

clonality :

Polyclonal

host :

Goat

reactivity :

Human. Predicted Reactivity: Monkey (at least 90% immunogen sequence identity)

sequence length :

925

specificity :

Human DDX58.

purity :

Immunoaffinity Purified

form :

Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide

concentration :

0.5 mg/ml

storage stability :

Store at -20 degree C. Minimize freezing and thawing.

tested application :

Immunohistochemistry (IHC - Paraffin)

app notes :

IHC-P (3.75 ug/ml)

other info1 :

Target Species: Human. Immunogen Description: Synthetic peptide from human DDX58 / RIG-1 / RIG-I. Percent identity by BLAST analysis: Human, Gorilla, Monkey (100%); Marmoset, Elephant (93%); Mouse, Bovine, Bat (86%).

other info2 :

Immunogen: DDX58 / RIG-1 / RIG-I antibody was raised against synthetic peptide from human DDX58 / RIG-1 / RIG-I. Percent identity by BLAST analysis: Human, Gorilla, Monkey (100%); Marmoset, Elephant (93%); Mouse, Bovine, Bat (86%).

products categories :

Family: DEAD box protein

products description :

EAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases which are implicated in a number of cellular processes involving RNA binding and alteration of RNA secondary structure. This gene encodes a protein containing RNA helicase-DEAD box protein motifs and a caspase recruitment domain (CARD). It is involved in viral double-stranded (ds) RNA recognition and the regulation of immune response.

ncbi gi num :

27881482

ncbi acc num :

NP_055129.2

ncbi gb acc num :

NM_014314.3

uniprot acc num :

O95786

ncbi mol weight :

101,377 Da

ncbi pathways :

Antiviral Mechanism By IFN-stimulated Genes Pathway (530760); Cytokine Signaling In Immune System Pathway (366171); Cytosolic DNA-sensing Pathway (125137); Cytosolic DNA-sensing Pathway (124833); Epstein-Barr Virus Infection Pathway (585562); Epstein-Barr Virus Infection Pathway (587115); Hepatitis B Pathway (694606); Hepatitis C Pathway (173973); Hepatitis C Pathway (173907); Herpes Simplex Infection Pathway (377873)

ncbi summary :

DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases which are implicated in a number of cellular processes involving RNA binding and alteration of RNA secondary structure. This gene encodes a protein containing RNA helicase-DEAD box protein motifs and a caspase recruitment domain (CARD). It is involved in viral double-stranded (ds) RNA recognition and the regulation of immune response. [provided by RefSeq, Jul 2008]

uniprot summary :

DDX58: an innate immune receptor which acts as a cytoplasmic sensor of viral nucleic acids and plays a major role in sensing viral infection and in the activation of a cascade of antiviral responses including the induction of type I interferons and proinflammatory cytokines. Its ligands include: 5 - triphosphorylated ssRNA and dsRNA and short dsRNA ( 1 kb in length). In addition to the 5 -triphosphate moiety, blunt-end base pairing at the 5 -end of the RNA is very essential. Overhangs at the non-triphosphorylated end of the dsRNA RNA have no major impact on its activity. A 3 overhang at the 5 triphosphate end decreases and any 5 overhang at the 5 triphosphate end abolishes its activity. Upon ligand binding it associates with mitochondria antiviral signaling protein MAVS which activates the IKK- related kinases TBK1 and IKBKE which phosphorylate interferon regulatory factors IRF3 and IRF7, in turn activating transcription of antiviral immunological genes, including IFN-alpha and IFN-beta. Detects both positive and negative strand RNA viruses including members of the families Paramyxoviridae: Human respiratory synctial virus and measles virus (MeV), Rhabdoviridae: vesicular stomatitis virus (VSV), Orthomyxoviridae: influenza A and B virus, Flaviviridae: Japanese encephalitis virus (JEV), hepatitis C virus (HCV), dengue virus (DENV) and west Nile virus (WNV). It also detects rotavirus and reovirus. Also involved in antiviral signaling in response to viruses containing a dsDNA genome such as Epstein-Barr virus (EBV). Detects dsRNA produced from non-self dsDNA by RNA polymerase III, such as Epstein-Barr virus-encoded RNAs (EBERs). May play important roles in granulocyte production and differentiation, bacterial phagocytosis and in the regulation of cell migration. Maintained as a monomer in an autoinhibited state. Upon viral dsRNA binding and conformation shift, homomultimerizes and interacts with MAVS. Interacts with DHX58, IKBKE, TBK1 and STING. Interacts (via CARD domain) with TRIM25 (via SPRY domain). Interacts with RNF135. Interacts with CYLD. Interacts with NLRC5; blocks the interaction of MAVS to DDX58. Interacts with SRC. Interacts with protein Z of Guanarito virus, Machupo virus, Junin arenavirus and Sabia virus. This interaction disrupts its interaction with MAVS, impeding downstream IRF3 and NF-kappa-B activation and resulting in decreased IFN-beta induction. Interacts (via CARD domain) with Human respiratory syncytial virus A non-structural protein 2 (NS2) and this interaction disrupts its interaction with MAVS, impeding downstream IRF3 activation. Present in vascular smooth cells. Belongs to the helicase family. RLR subfamily. 2 isoforms of the human protein are produced by alternative splicing. Protein type: EC 3.6.4.13; Hydrolase. Chromosomal Location of Human Ortholog: 9p12. Cellular Component: tight junction; cytoplasm; cytosol; actin cytoskeleton. Molecular Function: identical protein binding; protein binding; single-stranded RNA binding; zinc ion binding; double-stranded DNA binding; double-stranded RNA binding; helicase activity; ATP binding. Biological Process: response to exogenous dsRNA; viral reproduction; detection of virus; metabolic process; response to virus; positive regulation of transcription factor import into nucleus; positive regulation of interferon-beta production; innate immune response; positive regulation of transcription factor activity; positive regulation of transcription from RNA polymerase II promoter; positive regulation of defense response to virus by host; negative regulation of interferon type I production; regulation of cell migration; positive regulation of interferon-alpha production. Disease: Singleton-merten Syndrome 2

size1 :

0.05 mg

price1 :

495 USD

more info or order :

MyBioSource product webpage