Rabbit Polyclonal to Rat PDI | MyBioSource MBS247147 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Rabbit Polyclonal to Rat PDI

catalog :

MBS247147

quantity :

0.05 mL

price :

495 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat, dog, cow

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS247147

products type :

Antibody

products full name :

Rabbit Polyclonal to Rat PDI

products short name :

PDI

products name syn :

Anti-PDI Antibody IHC-plus; PDI; Rat PDI

other names :

Protein disulfide-isomerase; Protein disulfide-isomerase; protein disulfide-isomerase; p55; protocollagen hydroxylase; prolyl 4-hydroxylase subunit beta; collagen prolyl 4-hydroxylase beta; thyroid hormone-binding protein p55; glutathione-insulin transhydrogenase; cellular thyroid hormone-binding protein; protein disulfide isomerase-associated 1; protein disulfide isomerase/oxidoreductase; protein disulfide isomerase family A, member 1; procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), beta polypeptide; prolyl 4-hydroxylase, beta polypeptide; Cellular thyroid hormone-binding protein; Prolyl 4-hydroxylase subunit beta; p55

products gene name :

PDI

other gene names :

P4HB; P4HB; DSI; GIT; PDI; PHDB; PDIA1; PO4DB; PO4HB; PROHB; ERBA2L; P4Hbeta; ERBA2L; PDI; PDIA1; PO4DB; PDI

uniprot entry name :

PDIA1_HUMAN

clonality :

Polyclonal

host :

Rabbit

reactivity :

Bovine, Dog, Xenopus, Guinea pig, Hamster, Human, Monkey, Mouse, Pig, Rat, Sheep

sequence length :

508

specificity :

Detects ~58 kD protein.

purity :

Unpurified serum

form :

Antiserum

storage stability :

Short term 4 degree C, long term aliquot and store at -20 degree C, avoid freeze thaw cycles.

tested application :

Immunohistochemistry (IHC - Paraffin), Immunocytochemistry (ICC), Western Blot (WB), Immunoprecipitation (IP)

app notes :

ICC, IHC-P (1:100 - 1:250), IP, WB (1:4000)

other info1 :

Target: PDI. Target Species: Rat. Immunogen Description: Rat PDI synthetic peptide conjugated to KLH. Immunogen Species: PDI antibody was raised against Rat

other info2 :

Immunogen Type: Synthetic peptide - KLH conjugated. Immunogen: PDI antibody was raised against rat PDI synthetic peptide conjugated to KLH.

ncbi gi num :

2507460

ncbi acc num :

P07237.3

ncbi mol weight :

57,116 Da

ncbi pathways :

Cellular Responses To Stress Pathway (645258); Chylomicron-mediated Lipid Transport Pathway (106157); Collagen Biosynthesis And Modifying Enzymes Pathway (645289); Collagen Formation Pathway (645288); Detoxification Of Reactive Oxygen Species Pathway (1127552); Disease Pathway (530764); Extracellular Matrix Organization Pathway (576262); Hedgehog Ligand Biogenesis Pathway (1127536); Lipid Digestion, Mobilization, And Transport Pathway (106111); Lipoprotein Metabolism Pathway (106156)

ncbi summary :

This gene encodes the beta subunit of prolyl 4-hydroxylase, a highly abundant multifunctional enzyme that belongs to the protein disulfide isomerase family. When present as a tetramer consisting of two alpha and two beta subunits, this enzyme is involved in hydroxylation of prolyl residues in preprocollagen. This enzyme is also a disulfide isomerase containing two thioredoxin domains that catalyze the formation, breakage and rearrangement of disulfide bonds. Other known functions include its ability to act as a chaperone that inhibits aggregation of misfolded proteins in a concentration-dependent manner, its ability to bind thyroid hormone, its role in both the influx and efflux of S-nitrosothiol-bound nitric oxide, and its function as a subunit of the microsomal triglyceride transfer protein complex. [provided by RefSeq, Jul 2008]

uniprot summary :

PDIA1: This multifunctional protein catalyzes the formation, breakage and rearrangement of disulfide bonds. At the cell surface, seems to act as a reductase that cleaves disulfide bonds of proteins attached to the cell. May therefore cause structural modifications of exofacial proteins. Inside the cell, seems to form/rearrange disulfide bonds of nascent proteins. At high concentrations, functions as a chaperone that inhibits aggregation of misfolded proteins. At low concentrations, facilitates aggregation (anti-chaperone activity). May be involved with other chaperones in the structural modification of the TG precursor in hormone biogenesis. Also acts a structural subunit of various enzymes such as prolyl 4-hydroxylase and microsomal triacylglycerol transfer protein MTTP. Homodimer. Monomers and homotetramers may also occur. Also constitutes the structural subunit of prolyl 4-hydroxylase and of the microsomal triacylglycerol transfer protein MTTP in mammalian cells. Stabilizes both enzymes and retain them in the ER without contributing to the catalytic activity. Binds UBQLN1. Binds to CD4, and upon HIV-1 binding to the cell membrane, is part of a P4HB/PDI-CD4-CXCR4-gp120 complex. Belongs to the protein disulfide isomerase family. Protein type: EC 5.3.4.1; Nuclear receptor co-regulator; Isomerase; Oxidoreductase; Endoplasmic reticulum. Chromosomal Location of Human Ortholog: 17q25. Cellular Component: focal adhesion; endoplasmic reticulum lumen; endoplasmic reticulum; ER-Golgi intermediate compartment; plasma membrane; extracellular region; melanosome. Molecular Function: protein binding; enzyme binding; procollagen-proline 4-dioxygenase activity; protein heterodimerization activity; endopeptidase activity; protein disulfide isomerase activity. Biological Process: response to reactive oxygen species; extracellular matrix organization and biogenesis; protein folding; cell redox homeostasis; lipoprotein metabolic process; peptidyl-proline hydroxylation to 4-hydroxy-L-proline; proteolysis. Disease: Cole-carpenter Syndrome 1

size1 :

0.05 mL

price1 :

495 USD

more info or order :

MyBioSource product webpage