Goat Polyclonal to Human TTR / Transthyretin | MyBioSource MBS247117 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Goat Polyclonal to Human TTR / Transthyretin

catalog :

MBS247117

quantity :

0.25 mg

price :

530 USD

clonality :

polyclonal

host :

goat

conjugate :

nonconjugated

reactivity :

human, mouse

application :

ELISA, immunohistochemistry, enzyme immunoassay

more info or order :

MyBioSource product webpage

image

image 1 :

Anti-TTR / Transthyretin antibody IHC of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

image 2 :

Anti-TTR / Transthyretin antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

product information

catalog number :

MBS247117

products type :

Antibody

products full name :

Goat Polyclonal to Human TTR / Transthyretin

products short name :

[TTR / Transthyretin]

products name syn :

[TTR; ATTR; CTS1; CTS; HsT2651; PALB; Prealbumin; TBPA; Transthyretin; Carpal tunnel syndrome 1; Prealbumin; amyloidosis type I; Thyroxine-binding prealbumin; Human TTR; Transthyretin]

other names :

[transthyretin; Transthyretin; transthyretin; ATTR; carpal tunnel syndrome 1; thyroxine-binding prealbumin; epididymis luminal protein 111; prealbumin, amyloidosis type I; transthyretin; ATTR; Prealbumin; TBPA]

products gene name :

[TTR]

other gene names :

[TTR; TTR; CTS; CTS1; PALB; TBPA; HEL111; HsT2651; PALB]

uniprot entry name :

TTHY_HUMAN

clonality :

Polyclonal

host :

Goat

reactivity :

Human, Mouse

sequence length :

147

specificity :

Recognizes human Prealbumin.

purity :

Immunoaffinity Purified

form :

PBS, pH 7.2 0.05% sodium azide.

concentration :

1 mg/ml

storage stability :

Store at +4 degree C for shor-term only. Do not freeze.

tested application :

Immunohistochemistry (IHC - Paraffin), ELISA (EIA)

app notes :

IHC-P (5 ug/ml). Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by pathologist to confirm staining specificity. The optimal working concentration was determined to be 5 ug/ml. Optimal dilutions to be determined by the researcher.

image1 heading :

Immunohistochemistry (IHC)

image2 heading :

Immunohistochemistry (IHC)

other info1 :

Target: TTR/ Transthyretin. Immunogen Species: TTR / Transthyretin antibody was raised against Human. Antigen Type: Purified protein. Immunogen: TTR / Transthyretin antibody was raised against purified human Prealbumin.

other info2 :

. Disclaimer: Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application. Recommended Immunohistochemistry Protocol: The following protocol is a recommendation only, and MyBioSource, Inc. makes no guarantee of the results:. Tissue Preparation: . Formalin fixation and embedding in paraffin wax. Tissue Sectioning: . Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C. Deparaffinization: . Wash dry slides in 3 changes of xylene - 5 minutes each @ RT. Rehydration: . Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT. Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT. Wash slides in 1 change of 80% alcohol - 3 minutes @ RT. Rinse slides in gentle running distilled water - 5 minutes @ RT. Antigen retrieval: . Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes. Remove from heat and let stand at room temperature in buffer - 20 minutes. Rinse in 1X TBS with Tween (TBST) -1 minute @ RT. Immunostaining: . (Do not allow tissues to dry at any time during the staining procedure) . Apply a universal protein block - 20 minutes @ RT. Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT. Rinse slides in 1 X TBST - 1 minute @ RT. Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT. Rinse slides in 1X TBST -1 minute @ RT. Apply alkaline phosphatase streptavidin - 30 minutes @ RT. Rinse slides in 1X TBST -1 minute @ RT. Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT. Wash slides in distilled water - 1 minute @ RT. Dehydrate: . (This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB) . Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT. Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT. Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT. Wash slides in 3 changes of xylene - 1 minute each @ RT. Apply coverslip

products description :

Prealbumin, also known as transethrytin (TTR) is a carrier protein; it transports thyroid hormones in the plasma and cerebrospinal fluid, and also transports retinol (vitamin A) in the plasma. The protein consists of a tetramer of identical subunits. More than 80 different mutations in this gene have been reported; most mutations are related to amyloid deposition, affecting predominantly peripheral nerve and/or the heart, and a small portion of the gene mutations is non-amyloidogenic.

ncbi gi num :

4507725

ncbi acc num :

NP_000362.1

ncbi gb acc num :

NM_000371.3

uniprot acc num :

P02766

ncbi mol weight :

15,887 Da

ncbi pathways :

Amyloids Pathway (366238); Disease Pathway (530764); Diseases Associated With Visual Transduction Pathway (771581); Extracellular Matrix Organization Pathway (576262); FOXA2 And FOXA3 Transcription Factor Networks Pathway (137911); Non-integrin Membrane-ECM Interactions Pathway (833810); Retinoid Cycle Disease Events Pathway (771582); Retinoid Metabolism And Transport Pathway (187208); Signal Transduction Pathway (477114); The Canonical Retinoid Cycle In Rods (twilight Vision) Pathway (771585)

ncbi summary :

This gene encodes transthyretin, one of the three prealbumins including alpha-1-antitrypsin, transthyretin and orosomucoid. Transthyretin is a carrier protein; it transports thyroid hormones in the plasma and cerebrospinal fluid, and also transports retinol (vitamin A) in the plasma. The protein consists of a tetramer of identical subunits. More than 80 different mutations in this gene have been reported; most mutations are related to amyloid deposition, affecting predominantly peripheral nerve and/or the heart, and a small portion of the gene mutations is non-amyloidogenic. The diseases caused by mutations include amyloidotic polyneuropathy, euthyroid hyperthyroxinaemia, amyloidotic vitreous opacities, cardiomyopathy, oculoleptomeningeal amyloidosis, meningocerebrovascular amyloidosis, carpal tunnel syndrome, etc. [provided by RefSeq, Jan 2009]

uniprot summary :

TTR: Thyroid hormone-binding protein. Probably transports thyroxine from the bloodstream to the brain. Defects in TTR are the cause of amyloidosis transthyretin-related (AMYL-TTR). A hereditary generalized amyloidosis due to transthyretin amyloid deposition. Protein fibrils can form in different tissues leading to amyloid polyneuropathies, amyloidotic cardiomyopathy, carpal tunnel syndrome, systemic senile amyloidosis. The disease includes leptomeningeal amyloidosis that is characterized by primary involvement of the central nervous system. Neuropathologic examination shows amyloid in the walls of leptomeningeal vessels, in pia arachnoid, and subpial deposits. Some patients also develop vitreous amyloid deposition that leads to visual impairment (oculoleptomeningeal amyloidosis). Clinical features include seizures, stroke-like episodes, dementia, psychomotor deterioration, variable amyloid deposition in the vitreous humor. Defects in TTR are a cause of hyperthyroxinemia dystransthyretinemic euthyroidal (HTDE). It is a condition characterized by elevation of total and free thyroxine in healthy, euthyroid persons without detectable binding protein abnormalities. Defects in TTR are a cause of carpal tunnel syndrome type 1 (CTS1). It is a condition characterized by entrapment of the median nerve within the carpal tunnel. Symptoms include burning pain and paresthesias involving the ventral surface of the hand and fingers which may radiate proximally. Impairment of sensation in the distribution of the median nerve and thenar muscle atrophy may occur. This condition may be associated with repetitive occupational trauma, wrist injuries, amyloid neuropathies, rheumatoid arthritis. Belongs to the transthyretin family. Protein type: Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 18q12.1. Cellular Component: extracellular space; protein complex; cytoplasm; extracellular region. Molecular Function: identical protein binding; protein binding; protein heterodimerization activity; hormone activity. Biological Process: phototransduction, visible light; extracellular matrix organization and biogenesis; transport; retinol metabolic process; retinoid metabolic process. Disease: Hyperthyroxinemia, Dystransthyretinemic; Carpal Tunnel Syndrome; Amyloidosis, Hereditary, Transthyretin-related

size1 :

0.25 mg

price1 :

530 USD

more info or order :

MyBioSource product webpage