Mouse Monoclonal [clone B2M-01] (IgG2a) to Human B2M / Beta 2 Microglobulin | MyBioSource MBS246681 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Mouse Monoclonal [clone B2M-01] (IgG2a) to Human B2M / Beta 2 Microglobulin

catalog :

MBS246681

quantity :

0.05 mg

price :

530 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

[B2M-01]

reactivity :

human, dog, chicken, cow

application :

western blot, ELISA, immunohistochemistry, immunoprecipitation, flow cytometry, FACS, radioimmunoassay, enzyme immunoassay

more info or order :

MyBioSource product webpage

image

image 1 :

Anti-B2M / Beta 2 Microglobulin antibody IHC of human kidney, glomerulus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:50.

product information

catalog number :

MBS246681

products type :

Antibody

products full name :

Mouse Monoclonal [clone B2M-01] (IgG2a) to Human B2M / Beta 2 Microglobulin

products short name :

[B2M / Beta 2 Microglobulin]

products name syn :

[Anti-B2M / Beta 2 Microglobulin Antibody (clone B2M-01) IHC-plus; B2M; Beta 2 Microglobulin; Beta-2-microglobulin; Beta-2-microglobin; Human B2M; Beta 2 Microglobulin]

other names :

[beta-2-microglobulin; Beta-2-microglobulin; beta-2-microglobulin; beta-2-microglobin; beta chain of MHC class I molecules; beta-2-microglobulin]

products gene name :

[B2M]

other gene names :

[B2M; B2M]

uniprot entry name :

B2MG_HUMAN

clonality :

Monoclonal

isotype :

IgG2a

clone :

[B2M-01]

host :

Mouse

reactivity :

Reactivity: Human (tested or 100% immunogen sequence identity). Non-Reactivity: Mouse, Bovine, Dog, Rabbit, Chicken

sequence length :

119

specificity :

reacts with beta2-microglobulin (b2M) associated with cell-surfaceMHC Class I molecules and other membrane antigens as well as with solublebeta2-microglobulin. b2M is a 12 kDa Ig like glycoprotein expressed on lymphocytes,thymocytes, monocytes, granulocytes, platelets, endothelial cells and epithelial cells. It isabsent on erythrocytes.

purity :

Purified

form :

PBS containing 15 mM in sodium azide, pH 7.4

concentration :

1 mg/ml

storage stability :

Short term 4 degree C, long term aliquot and store at -20 degree C, avoid freeze thaw cycles.

tested application :

Immunohistochemistry (IHC - Paraffin), Western Blot (WB), Immunoprecipitation (IP), Flow Cytometry (FC/FACS), ELISA (EIA), Radioimmunoassay

app notes :

ELISA, Flo, IHC-P (1:50), IP, RIA, WB (2 - 4 ug/ml). Usage: For use in immunoprecipitation, this antibody coprecipitates b2-microglobulin (12 kDa) and HLA antigens(45 kDa) from the lysates of human lymphoid cells. Recommend U937 human histiocytic lymphoma cell line orRAJI human Burkitt lymphoma cell line as postitive control and EL4 mouse lymphoblastic lymphoma cell line as negative control for Western blot. Reducing and non-reducing conditions. Resuspend approx. 50 mil. cells in 1 ml cold Lysis buffer (1%laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including Proteaseinhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysate withnon-reducing/reducing Laemmli SDS-PAGE sample buffer. Recommend peripheral blood lymphocytes as positve control and DAUDI human Burkitt lymphoma cell line, or erythrocytes as negative control for flow cytometry. Recommend kidney glomeruli as postive control for IHC. For RIA, use at an assay dependent concentration. The dissociation constant of the antibody soluble b2-microglobulin is 1.5 x 10-8 mol/l as determined by competitive RIA.

image1 heading :

Immunohistochemistry (IHC)

other info1 :

Target Species: Human. Immunogen Description: Purified human beta2-microglobulin

other info2 :

Immunogen Type: Purified protein. Immunogen: B2M / Beta 2 Microglobulin antibody was raised against purified human beta2-microglobulin

products description :

B2M / Beta 2 Microglobulin is a serum protein found in association with the major histocompatibility complex (MHC) class I heavy chain on the surface of nearly all nucleated cells. The protein has a predominantly beta-pleated sheet structure that can form amyloid fibrils in some pathological conditions. The encoded antimicrobial protein displays antibacterial activity in amniotic fluid. A mutation in this gene has been shown to result in hypercatabolic hypoproteinemia.

ncbi gi num :

4757826

ncbi acc num :

NP_004039.1

ncbi gb acc num :

NM_004048.2

uniprot acc num :

P61769

ncbi mol weight :

13,715 Da

ncbi pathways :

Adaptive Immune System Pathway (366160); Amyloids Pathway (366238); Antigen Presentation: Folding, Assembly And Peptide Loading Of Class I MHC Pathway (366163); Antigen Processing And Presentation Pathway (83074); Antigen Processing And Presentation Pathway (485); Antigen Processing-Cross Presentation Pathway (477122); Class I MHC Mediated Antigen Processing Presentation Pathway (366161); Cytokine Signaling In Immune System Pathway (366171); DAP12 Interactions Pathway (685549); DAP12 Signaling Pathway (685550)

ncbi summary :

This gene encodes a serum protein found in association with the major histocompatibility complex (MHC) class I heavy chain on the surface of nearly all nucleated cells. The protein has a predominantly beta-pleated sheet structure that can form amyloid fibrils in some pathological conditions. The encoded antimicrobial protein displays antibacterial activity in amniotic fluid. A mutation in this gene has been shown to result in hypercatabolic hypoproteinemia.[provided by RefSeq, Aug 2014]

uniprot summary :

B2M: Component of the class I major histocompatibility complex (MHC). Involved in the presentation of peptide antigens to the immune system. Heterodimer of an alpha chain and a beta chain. Beta-2- microglobulin is the beta-chain of major histocompatibility complex class I molecules. Polymers of beta 2-microglobulin can be found in tissues from patients on long-term hemodialysis. Belongs to the beta-2-microglobulin family. Protein type: Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 15q21.1. Cellular Component: Golgi membrane; Golgi apparatus; extracellular space; phagocytic vesicle membrane; focal adhesion; membrane; early endosome membrane; endoplasmic reticulum lumen; cytoplasm; plasma membrane; extracellular region; MHC class I protein complex; external side of plasma membrane. Molecular Function: identical protein binding; protein binding. Biological Process: response to drug; regulation of immune response; viral reproduction; positive regulation of T cell mediated cytotoxicity; cytokine and chemokine mediated signaling pathway; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-independent; T cell differentiation in the thymus; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; regulation of defense response to virus by virus; retinal homeostasis; iron ion homeostasis; antigen processing and presentation of peptide antigen via MHC class I; response to cadmium ion; response to molecule of bacterial origin; innate immune response; antigen processing and presentation of exogenous peptide antigen via MHC class I; protein refolding; antigen processing and presentation of exogenous protein antigen via MHC class Ib, TAP-dependent. Disease: Hypoproteinemia, Hypercatabolic

size1 :

0.05 mg

price1 :

530 USD

more info or order :

MyBioSource product webpage