Mouse Monoclonal [clone 4C11] (IgG2a,k) to Human GALT | MyBioSource MBS246647 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Mouse Monoclonal [clone 4C11] (IgG2a,k) to Human GALT

catalog :

MBS246647

quantity :

0.05 mg

price :

605 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

[4C11]

reactivity :

human

application :

western blot, ELISA, immunohistochemistry, enzyme immunoassay

more info or order :

MyBioSource product webpage

image

image 1 :

Anti-GALT antibody IHC of human kidney, tubules. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.

image 2 :

GALT monoclonal antibody clone 4C11 Western blot of GALT expression in K-562.

image 3 :

Detection limit for recombinant GST tagged GALT is approximately 0.3 ng/ml as a capture antibody.

product information

catalog number :

MBS246647

products type :

Antibody

products full name :

Mouse Monoclonal [clone 4C11] (IgG2a,k) to Human GALT

products short name :

[GALT]

products name syn :

[Anti-GALT Antibody (clone 4C11) IHC-plus; GALT; Gal-1-P uridylyltransferase; Human GALT]

other names :

[galactose-1-phosphate uridylyltransferase isoform 1; Galactose-1-phosphate uridylyltransferase; galactose-1-phosphate uridylyltransferase; gal-1-P uridylyltransferase; galactose-1-phosphate uridyl transferase; UDP-glucose--hexose-1-phosphate uridylyltransferase; galactose-1-phosphate uridylyltransferase; UDP-glucose--hexose-1-phosphate uridylyltransferase]

products gene name :

[GALT]

other gene names :

[GALT; GALT; Gal-1-P uridylyltransferase]

uniprot entry name :

GALT_HUMAN

clonality :

Monoclonal

isotype :

IgG2a,k

clone :

[4C11]

host :

Mouse

reactivity :

Human

sequence length :

379

specificity :

Human GALT

purity :

Purified from ascites by Protein A.

form :

PBS, pH 7.4

concentration :

0.5 mg/mL

storage stability :

Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles.

tested application :

Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)

app notes :

IHC. IHC-Paraffin (10 ug/mL)Western Blot. ELISA . Optimal dilution to be determined by the researcher)

image1 heading :

Immunohistochemistry (IHC)

image2 heading :

Western Blot (WB)

image3 heading :

ELISA (EIA)

other info1 :

Immunogen Description: GALT (AAH15045, 1 a.a. ~ 380 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Immunogen: GALT antibody was raised against gALT (AAH15045, 1 a.a. ~ 380 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Antigen Type: Recombinant protein. Immunogen Species: GALT antibody was raised against human.

other info2 :

Disclaimer: Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application. Recommended Immunohistochemistry Protocol: The following protocol is a recommendation only, and MyBioSource, Inc. makes no guarantee of the results:. Tissue Preparation: . Formalin fixation and embedding in paraffin wax. Tissue Sectioning: . Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C. Deparaffinization: . Wash dry slides in 3 changes of xylene - 5 minutes each @ RT. Rehydration: . Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT. Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT. Wash slides in 1 change of 80% alcohol - 3 minutes @ RT. Rinse slides in gentle running distilled water - 5 minutes @ RT. Antigen retrieval: . Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes. Remove from heat and let stand at room temperature in buffer - 20 minutes. Rinse in 1X TBS with Tween (TBST) -1 minute @ RT. Immunostaining: . (Do not allow tissues to dry at any time during the staining procedure) . Apply a universal protein block - 20 minutes @ RT. Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT. Rinse slides in 1 X TBST - 1 minute @ RT. Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT. Rinse slides in 1X TBST -1 minute @ RT. Apply alkaline phosphatase streptavidin - 30 minutes @ RT. Rinse slides in 1X TBST -1 minute @ RT. Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT. Wash slides in distilled water - 1 minute @ RT. Dehydrate: . (This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB) . Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT. Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT. Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT. Wash slides in 3 changes of xylene - 1 minute each @ RT. Apply coverslip. Note: During shipment,small volumes of product will ocassionally become entrapped in the seal of the product vial. We recommend briefly centrifuging the vial to dislodge any liquid in the container's cap prior to opening. Warning: This reagent may contain sodium azide. The chemical, physical, and toxicologial properties of this material have not been thoughly investigated.Standard Laboratory Practices should be followed. Avoid skin and eye contact,inhalation, and ingestion. Sodium azide forms hydrazoic acid under acidic conditions and may react with lead or copper plubing to form highly explosive metal azides. On disposal, flush with large volumes of water to prevent accumulation.

products description :

Galactose-1-phosphate uridyl transferase (GALT) catalyzes the second step of the Leloir pathway of galactose metabolism, namely the conversion of UDP-glucose + galactose-1-phosphate to glucose-1-phosphate + UDP-galactose. The absence of this enzyme results in classic galactosemia in humans and can be fatal in the newborn period if lactose is not removed from the diet. The pathophysiology of galactosemia has not been clearly defined.

ncbi gi num :

22165416

ncbi acc num :

NP_000146.2

ncbi gb acc num :

NM_000155.3

uniprot acc num :

P07902

ncbi pathways :

Amino Sugar And Nucleotide Sugar Metabolism Pathway (82979); Amino Sugar And Nucleotide Sugar Metabolism Pathway (350); D-galactose Degradation V (Leloir Pathway) (1108782); Disease Pathway (530764); Galactose Catabolism Pathway (106219); Galactose Degradation, Leloir Pathway, Galactose = Alpha-D-glucose-1P (961175); Galactose Degradation, Leloir Pathway, Galactose = Alpha-D-glucose-1P (966887); Galactose Metabolism Pathway (82931); Galactose Metabolism Pathway (292); Glycogen Storage Diseases Pathway (1127581)

ncbi summary :

uniprot summary :

GALT: Defects in GALT are the cause of galactosemia (GALCT). Galactosemia is an inherited disorder of galactose metabolism that causes jaundice, cataracts, and mental retardation. Belongs to the galactose-1-phosphate uridylyltransferase type 1 family. Protein type: Motility/polarity/chemotaxis; Carbohydrate Metabolism - amino sugar and nucleotide sugar; Transferase; Carbohydrate Metabolism - galactose; EC 2.7.7.12. Chromosomal Location of Human Ortholog: 9p13. Cellular Component: Golgi apparatus; cytosol. Molecular Function: zinc ion binding; UDP-glucose:hexose-1-phosphate uridylyltransferase activity. Biological Process: galactose catabolic process; UDP-glucose catabolic process; carbohydrate metabolic process; galactose metabolic process; pathogenesis. Disease: Galactosemia

size1 :

0.05 mg

price1 :

605 USD

more info or order :

MyBioSource product webpage