antibody

Mouse Monoclonal [clone KN-03] (IgM) to Pig Kinesin Heavy Chain / KIF5B

MBS246591

0.05 mg

530 USD

monoclonal

mouse

nonconjugated

[KN-03]

human, mouse, rat

Antibody

Mouse Monoclonal [clone KN-03] (IgM) to Pig Kinesin Heavy Chain / KIF5B

[Kinesin Heavy Chain / KIF5B]

[Anti-Kinesin Heavy Chain / KIF5B Antibody (clone KN-03) IHC-plus; KIF5B; Kinesin 1 (110-120kD); KINH; KNS; Kinesin family member 5B; Kinesin heavy chain; KNS1; UKHC; Kinesin-1 heavy chain; Ubiquitous kinesin heavy chain; Pig Kinesin Heavy Chain; KIF5B]

[kinesin-1 heavy chain; Kinesin-1 heavy chain; kinesin-1 heavy chain; kinesin heavy chain; kinesin 1 (110-120kD); ubiquitous kinesin heavy chain; conventional kinesin heavy chain; epididymis secretory protein Li 61; kinesin family member 5B; Conventional kinesin heavy chain; Ubiquitous kinesin heavy chain; UKHC]

[KIF5B]

[KIF5B; KIF5B; KNS; KINH; KNS1; UKHC; HEL-S-61; KNS; KNS1; UKHC]

KINH_HUMAN

Monoclonal

IgM

[KN-03]

mouse

Human, Mouse, Pig, Rat

963

Recognizes heavy chain of conventional kinesin associated with vesicles and with lower affinity with denatured molecule. It stains Western blots of kinesin-enriched preparations. Epitope mapping (by limited proteolysis of partially purified porcine kinesin) followed by immunoblotting has revealed that antibodies KN-01, KN-02 and KN-03 react with different sets of fragments. The antibody KN-03 well recognizes kinesin bound to Taxol-stabilized microtubules

Ascites

PBS, 15 mM sodium azide, approx., pH 7.4. Sourced in Ascites.

1 mg/ml

Store at 4°C. Do not freeze.

IHC This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody.

IHC-Paraffin (10 ug/mL). ICC. (Optimal dilution to be determined by the researcher).

Immunohistochemistry (IHC)

Immunohistochemistry (IHC)

Immunogen Species: Kinesin Heavy Chain/ KIF5B antibody was raised against Pig. Antigen Type: Protein fraction. Immunogen: kinesin Heavy Chain/KIF5B antibody was raised against enriched fraction of porcine brain kinesin.

. Disclaimer: Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application. Recommended Immunohistochemistry Protocol: The following protocol is a recommendation only, and MyBioSource, Inc. makes no guarantee of the results:. Tissue Preparation: . Formalin fixation and embedding in paraffin wax. Tissue Sectioning: . Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C. Deparaffinization: . Wash dry slides in 3 changes of xylene - 5 minutes each @ RT. Rehydration: . Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT. Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT. Wash slides in 1 change of 80% alcohol - 3 minutes @ RT. Rinse slides in gentle running distilled water - 5 minutes @ RT. Antigen retrieval: . Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes. Remove from heat and let stand at room temperature in buffer - 20 minutes. Rinse in 1X TBS with Tween (TBST) -1 minute @ RT. Immunostaining: . (Do not allow tissues to dry at any time during the staining procedure) . Apply a universal protein block - 20 minutes @ RT. Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT. Rinse slides in 1 X TBST - 1 minute @ RT. Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT. Rinse slides in 1X TBST -1 minute @ RT. Apply alkaline phosphatase streptavidin - 30 minutes @ RT. Rinse slides in 1X TBST -1 minute @ RT. Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT. Wash slides in distilled water - 1 minute @ RT. Dehydrate: . (This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB) . Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT. Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT. Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT. Wash slides in 3 changes of xylene - 1 minute each @ RT. Apply coverslip

4758648

NP_004512.1

NM_004521.2

P33176

109,685 Da

Adaptive Immune System Pathway (366160); Dopaminergic Synapse Pathway (469199); Dopaminergic Synapse Pathway (469185); Factors Involved In Megakaryocyte Development And Platelet Production Pathway (187196); Hemostasis Pathway (106028); Immune System Pathway (106386); Insulin Signaling Pathway (198845); Insulin Processing Pathway (105903); Kinesins Pathway (187197); MHC Class II Antigen Presentation Pathway (645290)

KIF5B: Microtubule-dependent motor required for normal distribution of mitochondria and lysosomes. Belongs to the kinesin-like protein family. Kinesin subfamily. Protein type: Motor; Microtubule-binding. Chromosomal Location of Human Ortholog: 10p11.22. Cellular Component: ciliary rootlet; microtubule; kinesin complex; neuron projection; membrane; endocytic vesicle; perinuclear region of cytoplasm; cytoplasm; microtubule organizing center; vesicle. Molecular Function: protein binding; plus-end-directed microtubule motor activity; microtubule binding; ATPase activity; microtubule motor activity; ATP binding. Biological Process: axon guidance; regulation of membrane potential; cellular protein metabolic process; positive regulation of potassium ion transport; vesicle transport along microtubule; positive regulation of synaptic transmission, GABAergic; cytoplasm organization and biogenesis; cytoskeleton-dependent intracellular transport; microtubule-based movement

0.05 mg

530 USD