antibody

Mouse Monoclonal [clone A6.4.12] (IgG1) to Human PARP1 / PARP

MBS246444

0.05 mg

530 USD

monoclonal

mouse

nonconjugated

[A6.4.12]

western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation, enzyme immunoassay, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

Antibody

Mouse Monoclonal [clone A6.4.12] (IgG1) to Human PARP1 / PARP

[PARP1 / PARP]

[Anti-PARP1 / PARP Antibody (clone A6.4.12) IHC-plus; PARP1; ADPRT; ADP-ribosyltransferase NAD(+); Adp-ribosyltransferase; ADPRT1; ARTD1; ADPRT 1; Poly(ADP-ribose) synthetase; Poly(ADP-ribosyl)transferase; PARP; Poly [ADP-ribose] polymerase 1; PADPRT-1; PARP-1; Poly (ADP-ribose) polymerase 1; Human PARP1; PARP]

[poly; Poly [ADP-ribose] polymerase 1; poly [ADP-ribose] polymerase 1; poly [ADP-ribose] polymerase 1; poly(ADP-ribose) polymerase; poly(ADP-ribose) synthetase; poly[ADP-ribose] synthase 1; poly(ADP-ribosyl)transferase; ADP-ribosyltransferase NAD(+); NAD(+) ADP-ribosyltransferase 1; poly (ADP-ribose) polymerase family, member 1; ADP-ribosyltransferase diphtheria toxin-like 1; ADP-ribosyltransferase (NAD+; poly (ADP-ribose) polymerase); poly (ADP-ribose) polymerase 1; ADP-ribosyltransferase diphtheria toxin-like 1; ARTD1; NAD(+) ADP-ribosyltransferase 1; ADPRT 1; Poly[ADP-ribose] synthase 1]

[PARP1]

[PARP]

[PARP1; PARP1; PARP; PPOL; ADPRT; ARTD1; ADPRT1; PARP-1; ADPRT 1; pADPRT-1; ADPRT; PPOL; PARP-1; ARTD1; ADPRT 1]

PARP1_HUMAN

Monoclonal

IgG1

[A6.4.12]

Mouse

Human

1014

Recognizes poly (ADP-ribose) polymerase 1 (PARP-1), a ~116kD nuclear enzyme cleaved during apoptosis. PARP-1, a caretaker enzyme, is involved in DNA damage repair, plays roles in diabetes pathophysiology and tumor proliferation.). As well as protecting cells from genomic instability, PARP-1 is involved in the development of both inflammatory and immune responses, and cell death by apoptosis and necrosis. Targets PARP-1, an enzyme which represents a promising target for new developments in therapeutic treatment of immune mediated diseases. PARP-1 has considerable potential for delivering selective tumor cell killing while sparing normal cells.

Protein G Purified

PBS, 0.09% Sodium Azide

1 mg/ml

Store at 4°C or at -20°C. Store undiluted. Avoid freeze-thaw cycles. Microcentrifugation recommended if solution contains precipitate.

Immunohistochemistry (IHC) - Paraffin/Frozen, Western Blot (WB), Immunoprecipitation (IP), ELISA (EIA), Immunofluorescence (IF)

Immunohistochemistry: MBS246444 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration was determined to be 10 ug/ml. IHC - Paraffin (10 µg/ml). ELISA (1:1000 - 1:5000)

Immunohistochemistry (IHC)

Immunohistochemistry (IHC)

Immunohistochemistry (IHC)

Antigen Type: Purified protein. Immunogen: PARP1 antibody was raised against human PARP. Immunogen Species: PARP1 antibody was raised against Human.

Disclaimer: Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application. Recommended Immunohistochemistry Protocol: The following protocol is a recommendation only, and MyBioSource, Inc. makes no guarantee of the results:. Tissue Preparation: . Formalin fixation and embedding in paraffin wax. Tissue Sectioning: . Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C. Deparaffinization: . Wash dry slides in 3 changes of xylene - 5 minutes each @ RT. Rehydration: . Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT. Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT. Wash slides in 1 change of 80% alcohol - 3 minutes @ RT. Rinse slides in gentle running distilled water - 5 minutes @ RT. Antigen retrieval: . Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes. Remove from heat and let stand at room temperature in buffer - 20 minutes. Rinse in 1X TBS with Tween (TBST) -1 minute @ RT. Immunostaining: . (Do not allow tissues to dry at any time during the staining procedure) . Apply a universal protein block - 20 minutes @ RT. Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT. Rinse slides in 1 X TBST - 1 minute @ RT. Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT. Rinse slides in 1X TBST -1 minute @ RT. Apply alkaline phosphatase streptavidin - 30 minutes @ RT. Rinse slides in 1X TBST -1 minute @ RT. Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT. Wash slides in distilled water - 1 minute @ RT. Dehydrate: . (This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB) . Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT. Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT. Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT. Wash slides in 3 changes of xylene - 1 minute each @ RT. Apply coverslip

156523968

NP_001609.2

NM_001618.3

P09874

113,084 Da

BER Complex Pathway (413429); BER Complex Pathway (468377); Base Excision Repair Pathway (83043); Base Excision Repair Pathway (451); Caspase Cascade In Apoptosis Pathway (137974); Corticotropin-releasing Hormone Pathway (920957); Disease Pathway (530764); Downregulation Of SMAD2/3:SMAD4 Transcriptional Activity Pathway (645266); FAS Pathway And Stress Induction Of HSP Regulation (198894); Gene Expression Pathway (105937)

This gene encodes a chromatin-associated enzyme, poly(ADP-ribosyl)transferase, which modifies various nuclear proteins by poly(ADP-ribosyl)ation. The modification is dependent on DNA and is involved in the regulation of various important cellular processes such as differentiation, proliferation, and tumor transformation and also in the regulation of the molecular events involved in the recovery of cell from DNA damage. In addition, this enzyme may be the site of mutation in Fanconi anemia, and may participate in the pathophysiology of type I diabetes. [provided by RefSeq, Jul 2008]

PARP1: Involved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks. Mediates the poly(ADP- ribosyl)ation of APLF and CHFR. Positively regulates the transcription of MTUS1 and negatively regulates the transcription of MTUS2/TIP150. With EEF1A1 and TXK, forms a complex that acts as a T-helper 1 (Th1) cell-specific transcription factor and binds the promoter of IFN-gamma to directly regulate its transcription, and is thus involved importantly in Th1 cytokine production. Component of a base excision repair (BER) complex, containing at least XRCC1, PARP2, POLB and LRIG3. Homo- and heterodimer with PARP2. Interacts with PARP3, APTX and SRY. The SWAP complex consists of NPM1, NCL, PARP1 and SWAP70. Interacts with TIAM2 and ZNF423. Interacts (when poly-ADP- ribosylated) with CHD1L. Interacts with the DNA polymerase alpha catalytic subunit POLA1; this interaction functions as part of the control of replication fork progression. Interacts with EEF1A1, RNF4 and TXK. Protein type: Nuclear receptor co-regulator; EC 2.4.2.30; DNA repair, damage; Nucleolus; Nuclear envelope; Transferase. Chromosomal Location of Human Ortholog: 1q41-q42. Cellular Component: nucleoplasm; transcription factor complex; membrane; nucleolus; nuclear envelope; nucleus. Molecular Function: identical protein binding; protein binding; enzyme binding; DNA binding; zinc ion binding; protein N-terminus binding; transcription factor binding; protein kinase binding; NAD binding; NAD+ ADP-ribosyltransferase activity. Biological Process: transcription from RNA polymerase II promoter; transcription initiation from RNA polymerase II promoter; macrophage differentiation; transcription, DNA-dependent; DNA damage response, detection of DNA damage; negative regulation of transcription from RNA polymerase II promoter; DNA repair; protein autoprocessing; protein amino acid ADP-ribosylation; cellular response to insulin stimulus; base-excision repair; double-strand break repair; transforming growth factor beta receptor signaling pathway; positive regulation of transcription from RNA polymerase II promoter; gene expression; regulation of growth rate; telomere maintenance

0.05 mg

530 USD