Mouse Monoclonal (IgG1) to Human MUC16 / CA125 | MyBioSource MBS246428 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Mouse Monoclonal (IgG1) to Human MUC16 / CA125

catalog :

MBS246428

quantity :

0.05 mg

price :

530 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

reactivity :

human

application :

ELISA, immunohistochemistry, enzyme immunoassay

more info or order :

MyBioSource product webpage

image

image 1 :

Anti-MUC16 / CA-125 antibody IHC of human uterus, endometrium. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

product information

catalog number :

MBS246428

products type :

Antibody

products full name :

Mouse Monoclonal (IgG1) to Human MUC16 / CA125

products short name :

[MUC16 / CA125]

products name syn :

[Anti-MUC16/CA125 Antibody; MUC16; CA125 ovarian cancer antigen; CA125; CA-125; MUC-16; Mucin-16]

other names :

[mucin-16; Mucin-16; mucin-16; CA-125; MUC-16; CA125 ovarian cancer antigen; ovarian carcinoma antigen CA125; ovarian cancer-related tumor marker CA125; mucin 16, cell surface associated; Ovarian cancer-related tumor marker CA125; CA-125; Ovarian carcinoma antigen CA125]

products gene name :

[MUC16]

products gene name syn :

[CA125]

other gene names :

[MUC16; MUC16; CA125; CA125; MUC-16; CA-125]

uniprot entry name :

MUC16_HUMAN

clonality :

Monoclonal

isotype :

IgG1

host :

Mouse

reactivity :

Human

sequence length :

14507

specificity :

Recognizes CA 125

purity :

Protein A Purified

form :

10 mM Tris buffer, pH 7.5, 150 mM sodium chloride, 0.05% sodium azide.

concentration :

1 mg/ml

storage stability :

Upon arrival: Long term: -20 degree C; Short term: +4 degree C; Avoid freeze-thaw cycles.

tested application :

Immunohistochemistry (IHC - Paraffin), ELISA (EIA)

app notes :

IHC-P (5 ug/ml). Optimal dilution to be determined by the researcher

image1 heading :

Immunohistochemistry (IHC)

other info1 :

Immunogen Species: MUC16/CA125 antibody was raised against Human. Immunogen: MUC16 / CA125 antibody was raised against affinity pure CA 125 antigen (human).

other info2 :

Disclaimer: Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application. Recommended Immunohistochemistry Protocol: The following protocol is a recommendation only, and MyBioSource, Inc. makes no guarantee of the results:. Tissue Preparation: . Formalin fixation and embedding in paraffin wax. Tissue Sectioning: . Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C. Deparaffinization: . Wash dry slides in 3 changes of xylene - 5 minutes each @ RT. Rehydration: . Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT. Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT. Wash slides in 1 change of 80% alcohol - 3 minutes @ RT. Rinse slides in gentle running distilled water - 5 minutes @ RT. Antigen retrieval: . Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes. Remove from heat and let stand at room temperature in buffer - 20 minutes. Rinse in 1X TBS with Tween (TBST) -1 minute @ RT. Immunostaining: . (Do not allow tissues to dry at any time during the staining procedure) . Apply a universal protein block - 20 minutes @ RT. Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT. Rinse slides in 1 X TBST - 1 minute @ RT. Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT. Rinse slides in 1X TBST -1 minute @ RT. Apply alkaline phosphatase streptavidin - 30 minutes @ RT. Rinse slides in 1X TBST -1 minute @ RT. Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT. Wash slides in distilled water - 1 minute @ RT. Dehydrate: . (This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB) . Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT. Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT. Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT. Wash slides in 3 changes of xylene - 1 minute each @ RT. Apply coverslip

products description :

Also commonly known as Ovarian Cancer Antigen CA-125.

ncbi gi num :

83367077

ncbi acc num :

NP_078966.2

ncbi gb acc num :

NM_024690.2

uniprot acc num :

Q8WXI7

ncbi mol weight :

2,353,428 Da

ncbi pathways :

Metabolism Of Proteins Pathway (106230); O-linked Glycosylation Pathway (1127679); O-linked Glycosylation Of Mucins Pathway (530747); Post-translational Protein Modification Pathway (161012); Termination Of O-glycan Biosynthesis Pathway (530748)

uniprot summary :

MUC16: Thought to provide a protective, lubricating barrier against particles and infectious agents at mucosal surfaces. Protein type: Cell adhesion; Membrane protein, peripheral; Membrane protein, integral. Chromosomal Location of Human Ortholog: 19p13.2. Cellular Component: extracellular space; extrinsic to membrane; Golgi lumen; integral to membrane; plasma membrane; vesicle. Molecular Function: protein binding. Biological Process: protein amino acid O-linked glycosylation; cellular protein metabolic process; O-glycan processing; cell adhesion; post-translational protein modification

size1 :

0.05 mg

price1 :

530 USD

more info or order :

MyBioSource product webpage