Mouse Monoclonal [clone 2H4] (IgG2b,k) to Human TNFRSF18 / GITR | MyBioSource MBS246415 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Mouse Monoclonal [clone 2H4] (IgG2b,k) to Human TNFRSF18 / GITR

catalog :

MBS246415

quantity :

0.05 mg

price :

595 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

[2H4]

reactivity :

human, mouse

application :

western blot, ELISA, immunohistochemistry, enzyme immunoassay

more info or order :

MyBioSource product webpage

image

image 1 :

Anti-TNFRSF18 antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

image 2 :

TNFRSF18 monoclonal antibody, clone 2H4. Western blot of TNFRSF18 expression in NIH/3T3.

image 3 :

TNFRSF18 monoclonal antibody, clone 2H4. Western blot of TNFRSF18 expression in HeLa nuclear extracts.

product information

catalog number :

MBS246415

products type :

Antibody

products full name :

Mouse Monoclonal [clone 2H4] (IgG2b,k) to Human TNFRSF18 / GITR

products short name :

[TNFRSF18 / GITR]

products name syn :

[TNFRSF18; AITR; CD357; CD357 antigen; GITR; GITR-D]

other names :

[tumor necrosis factor receptor superfamily member 18 isoform 1; Tumor necrosis factor receptor superfamily member 18; tumor necrosis factor receptor superfamily member 18; activation-inducible TNFR family receptor; glucocorticoid-induced TNFR-related protein; TNF receptor superfamily activation-inducible protein; tumor necrosis factor receptor superfamily, member 18; Activation-inducible TNFR family receptor; Glucocorticoid-induced TNFR-related protein; CD_antigen: CD357]

products gene name :

[TNFRSF18]

products gene name syn :

[GITR]

other gene names :

[TNFRSF18; TNFRSF18; AITR; GITR; CD357; GITR-D; AITR; GITR]

uniprot entry name :

TNR18_HUMAN

clonality :

Monoclonal

isotype :

IgG2b,k

clone :

[2H4]

host :

Mouse

reactivity :

Human, Mouse

sequence length :

241

specificity :

Human TNFRSF18

purity :

Purified from ascites by Protein A

form :

PBS, pH 7.4.

concentration :

0.5 mg/ml

storage stability :

Store at -20 degree C or lower. Aliquot to avoid repeated freezing and thawing.

tested application :

Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)

app notes :

ELISA. IHC-P (5 ug/ml). WB (1:500 - 1:1000). (optimal dilution to be determined by the researcher). Usage: Western Blot: Cell lysate, Cell lysate, Recombinant protein.

image1 heading :

Immunohistochemistry (IHC)

image2 heading :

Western Blot (WB)

image3 heading :

Western Blot (WB)

other info1 :

Family/Subfamily: TNF Receptor/ not assigned-TNF Receptor. Target Species: Human. Immunogen Species: TNFRSF18 / GITR antibody was raised against Human. Immunogen: TNFRSF18 / GITR antibody was raised against recombinant protein encoding amino acids 26-115 of human TNFRSF18. Antigen Type: Recombinant protein

other info2 :

. Disclaimer: Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application. Recommended Immunohistochemistry Protocol: The following protocol is a recommendation only, and MyBioSource, Inc. makes no guarantee of the results:. Tissue Preparation: . Formalin fixation and embedding in paraffin wax. Tissue Sectioning: . Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C. Deparaffinization: . Wash dry slides in 3 changes of xylene - 5 minutes each @ RT. Rehydration: . Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT. Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT. Wash slides in 1 change of 80% alcohol - 3 minutes @ RT. Rinse slides in gentle running distilled water - 5 minutes @ RT. Antigen retrieval: . Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes. Remove from heat and let stand at room temperature in buffer - 20 minutes. Rinse in 1X TBS with Tween (TBST) -1 minute @ RT. Immunostaining: . (Do not allow tissues to dry at any time during the staining procedure) . Apply a universal protein block - 20 minutes @ RT. Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT. Rinse slides in 1 X TBST - 1 minute @ RT. Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT. Rinse slides in 1X TBST -1 minute @ RT. Apply alkaline phosphatase streptavidin - 30 minutes @ RT. Rinse slides in 1X TBST -1 minute @ RT. Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT. Wash slides in distilled water - 1 minute @ RT. Dehydrate: . (This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB) . Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT. Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT. Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT. Wash slides in 3 changes of xylene - 1 minute each @ RT. Apply coverslip . Note: During shipment,small volumes of product will ocassionally become entrapped in the seal of the product vial. We recommend briefly centrifuging the vial to dislodge any liquid in the container's cap prior to opening. Warning: This reagent may contain sodium azide. The chemical, physical, and toxicologial properties of this material have not been thoughly investigated.Standard Laboratory Practices should be followed. Avoid skin and eye contact,inhalation, and ingestion. Sodium azide forms hydrazoic acid under acidic conditions and may react with lead or copper plubing to form highly explosive metal azides. On disposal, flush with large volumes of water to prevent accumulation.

products categories :

Family: TNF Receptor

ncbi gi num :

4759246

ncbi acc num :

NP_004186.1

ncbi gb acc num :

NM_004195.2

uniprot acc num :

Q9Y5U5

ncbi pathways :

Cytokine-cytokine Receptor Interaction Pathway (83051); Cytokine-cytokine Receptor Interaction Pathway (460); Downstream Signaling In Naive CD8+ T Cells Pathway (138018)

ncbi summary :

This gene encodes a member of the TNF-receptor superfamily. The encoded receptor has been shown to have increased expression upon T-cell activation, and it is thought to play a key role in dominant immunological self-tolerance maintained by CD25(+)CD4(+) regulatory T cells. Knockout studies in mice also suggest the role of this receptor is in the regulation of CD3-driven T-cell activation and programmed cell death. Three alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. [provided by RefSeq, Feb 2011]

uniprot summary :

TNFRSF18: Receptor for TNFSF18. Seems to be involved in interactions between activated T-lymphocytes and endothelial cells and in the regulation of T-cell receptor-mediated cell death. Mediated NF-kappa-B activation via the TRAF2/NIK pathway. 3 isoforms of the human protein are produced by alternative splicing. Protein type: Receptor, cytokine; Apoptosis. Chromosomal Location of Human Ortholog: 1p36.3. Cellular Component: integral to plasma membrane; extracellular region; external side of plasma membrane. Molecular Function: tumor necrosis factor receptor activity. Biological Process: tumor necrosis factor-mediated signaling pathway; apoptosis; signal transduction; negative regulation of apoptosis

size1 :

0.05 mg

price1 :

595 USD

more info or order :

MyBioSource product webpage