Rat Monoclonal [clone 6D12.H10.E4] (IgG) to Human GDF15 | MyBioSource MBS246403 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Rat Monoclonal [clone 6D12.H10.E4] (IgG) to Human GDF15

catalog :

MBS246403

quantity :

0.05 mg

price :

530 USD

clonality :

monoclonal

host :

rat

conjugate :

nonconjugated

clone name :

[6D12.H10.E4]

reactivity :

human, mouse

application :

western blot, ELISA, immunohistochemistry, immunohistochemistry - paraffin section

more info or order :

MyBioSource product webpage

image

image 1 :

Anti-GDF15 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 10 ug/ml.

product information

catalog number :

MBS246403

products type :

Antibody

products full name :

Rat Monoclonal [clone 6D12.H10.E4] (IgG) to Human GDF15

products short name :

[GDF15]

products name syn :

[Anti-GDF15 Antibody (clone 6D12.H10.E4) IHC-plus; GDF15; MIC1; MIC-1; NAG-1; NRG-1; NSAID-regulated gene 1 protein; PLAB; NSAID-activated gene 1 protein; Placental TGF-beta; PTGFB; GDF-15; PTGF-beta; Human GDF15]

other names :

[growth/differentiation factor 15; Growth/differentiation factor 15; growth/differentiation factor 15; NRG-1; PTGF-beta; placental TGF-beta; NSAID-activated gene 1 protein; NSAID-regulated gene 1 protein; prostate differentiation factor; macrophage inhibitory cytokine 1; placental bone morphogenetic protein; NSAID (nonsteroidal anti-inflammatory drug)-activated protein 1; growth differentiation factor 15; Macrophage inhibitory cytokine 1; MIC-1; NSAID-activated gene 1 protein; NAG-1; NSAID-regulated gene 1 protein; NRG-1; Placental TGF-beta; Placental bone morphogenetic protein; Prostate differentiation factor]

products gene name :

[GDF15]

other gene names :

[GDF15; GDF15; PDF; MIC1; PLAB; MIC-1; NAG-1; PTGFB; GDF-15; MIC1; PDF; PLAB; PTGFB; GDF-15; MIC-1; NAG-1; NRG-1]

uniprot entry name :

GDF15_HUMAN

clonality :

Monoclonal

isotype :

IgG

clone :

[6D12.H10.E4]

host :

Rat

reactivity :

Human, Mouse

sequence length :

308

specificity :

This antibody reacts with the C-terminus of endogenous NAG-1 protein from human tissues. A BLAST analysis suggests reactivity with NAG-1 from chimpanzee and macaque based on a 100% homology. Partial reactivity is expected against rat based on an 86% homology with the immunizing sequence. Cross-reactivity with NAG-1 from other sources has not been determined.

purity :

Protein G Purified

form :

0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide.

concentration :

1 mg/ml

storage stability :

Store vial at -20°C prior to opening. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles. Centrifuge product if not completely clear after standing at room temperature. This product is stable several weeks at 4°C as an undiluted liquid.

tested application :

Immunohistochemistry (IHC), Immunohistochemistry-Paraffin (IHC-P), Western Blot (WB), ELISA

app notes :

IHC-Paraffin (10 ug/ml). Western Blot (1:1000). Optimal dilution to be determined by the researcher. Usage Summary: This protein G purified antibody is suitable for ELISA and western blotting of human NAG-1 protein. For detection of NAG-1 in human serum, a sandwich ELISA is suggested using this antibody in combination with anti-NAG-1/GDF15 (N-terminal), H variant or D variant specific antibodies. Specific conditions for reactivity should be optimized by the end user. Expect bands in Western blots of native protein of approximately 13 and 26 kD in size corresponding to NAG-1 monomer and dimer, respectively using the appropriate cell lysate or extract.

image1 heading :

Immunohistochemistry (IHC)

other info1 :

Family/Subfamily: TGF beta/ not assigned-TGF beta. Immunogen Species: GDF15 antibody raised against Mouse. Antigen Type: Recombinant protein. Immunogen: GDF15 antibody was raised against this Protein-A purified antibody was prepared by repeated immunizations with an MBP-tagged recombinant protein produced in E Coli corresponding to full-length mouse NAG-1 protein. Cross reactivity to MBP was removed via cross-adsorption chromatography.

other info2 :

Disclaimer: Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application. Recommended Immunohistochemistry Protocol: The following protocol is a recommendation only, and MyBioSource, Inc. makes no guarantee of the results:. Tissue Preparation: . Formalin fixation and embedding in paraffin wax. Tissue Sectioning: . Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C. Deparaffinization: . Wash dry slides in 3 changes of xylene - 5 minutes each @ RT. Rehydration: . Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT. Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT. Wash slides in 1 change of 80% alcohol - 3 minutes @ RT. Rinse slides in gentle running distilled water - 5 minutes @ RT. Antigen retrieval: . Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes. Remove from heat and let stand at room temperature in buffer - 20 minutes. Rinse in 1X TBS with Tween (TBST) -1 minute @ RT. Immunostaining: . (Do not allow tissues to dry at any time during the staining procedure) . Apply a universal protein block - 20 minutes @ RT. Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT. Rinse slides in 1 X TBST - 1 minute @ RT. Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT. Rinse slides in 1X TBST -1 minute @ RT. Apply alkaline phosphatase streptavidin - 30 minutes @ RT. Rinse slides in 1X TBST -1 minute @ RT. Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT. Wash slides in distilled water - 1 minute @ RT. Dehydrate: . (This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB) . Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT. Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT. Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT. Wash slides in 3 changes of xylene - 1 minute each @ RT. Apply coverslip

products categories :

Family: TGF beta

ncbi gi num :

153792495

ncbi acc num :

NP_004855.2

ncbi gb acc num :

NM_004864.2

uniprot acc num :

Q99988

ncbi pathways :

Direct P53 Effectors Pathway (137939)

ncbi summary :

Bone morphogenetic proteins (e.g., BMP9; MIM 605120) are members of the transforming growth factor-beta (see TGFB1; MIM 190180) superfamily and regulate tissue differentiation and maintenance. They are synthesized as precursor molecules that are processed at a dibasic cleavage site to release C-terminal domains containing a characteristic motif of 7 conserved cysteines in the mature protein.[supplied by OMIM, Oct 2009]

uniprot summary :

GDF15: disulfide-linked. Belongs to the TGF-beta family. Protein type: Secreted, signal peptide; Secreted; Cytokine. Chromosomal Location of Human Ortholog: 19p13.11. Cellular Component: extracellular space; extracellular region. Molecular Function: growth factor activity; cytokine activity; transforming growth factor beta receptor binding. Biological Process: regulation of apoptosis; cell-cell signaling; transforming growth factor beta receptor signaling pathway; regulation of MAPKKK cascade; signal transduction; cell development

size1 :

0.05 mg

price1 :

530 USD

more info or order :

MyBioSource product webpage