Mouse Monoclonal [clone 139H2] (IgG1) to Human EMA / MUC1 | MyBioSource MBS246154 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

MyBioSource

product type :

antibody

product name :

Mouse Monoclonal [clone 139H2] (IgG1) to Human EMA / MUC1

catalog :

MBS246154

quantity :

0.05 mg

price :

495 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

139H2

reactivity :

human

application :

immunohistochemistry, flow cytometry, FACS, enzyme immunoassay

product information

catalog number :

MBS246154

products type :

Antibody

products full name :

Mouse Monoclonal [clone 139H2] (IgG1) to Human EMA / MUC1

products short name :

EMA / MUC1

products name syn :

Anti-EMA / MUC1 Antibody (clone 139H2) IHC-plus; MUC1; CA15-3; CD227; Carcinoma-associated mucin; CD227 antigen; DF3 antigen; Episialin; Epithelial membrane antigen; H23 antigen; H23AG; Krebs von den Lungen-6; MAM6; MUC1/ZD; MUC-1/SEC; Mucin-1; Pem; KL-6; MUC-1/X; Tumor-associated mucin; Human EMA; MUC1

other names :

mucin-1 isoform 1; Mucin-1; mucin-1; episialin; DF3 antigen; H23 antigen; cancer antigen 15-3; krebs von den Lungen-6; mucin 1, transmembrane; carcinoma-associated mucin; polymorphic epithelial mucin; peanut-reactive urinary mucin; tumor associated epithelial mucin; breast carcinoma-associated antigen DF3; tumor-associated epithelial membrane antigen; Medullary cystic kidney disease, autosomal dominant; mucin 1, cell surface associated; Breast carcinoma-associated antigen DF3; Cancer antigen 15-3; CA 15-3; Carcinoma-associated mucin; Episialin; H23AG; Krebs von den Lungen-6; KL-6; PEMT; Peanut-reactive urinary mucin; PUM; Polymorphic epithelial mucin; PEM; Tumor-associated epithelial membrane antigen; EMA; Tumor-associated mucin; CD_antigen: CD227Cleaved into the following 2 chains:Mucin-1 subunit alpha; MUC1-NT; MUC1-alpha; Mucin-1 subunit beta; MUC1-betaAlternative name(s):MUC1-CT

products gene name :

EMA

products gene name syn :

MUC1

other gene names :

MUC1; MUC1; EMA; MCD; PEM; PUM; KL-6; MAM6; MCKD; PEMT; CD227; H23AG; MCKD1; MUC-1; ADMCKD; ADMCKD1; CA 15-3; MUC-1/X; MUC1/ZD; MUC-1/SEC; PUM; MUC-1; CA 15-3; KL-6; PUM; PEM; EMA; MUC1-NT; MUC1-alpha; MUC1-beta

uniprot entry name :

MUC1_HUMAN

clonality :

Monoclonal

isotype :

IgG1

clone :

139H2

host :

Mouse

reactivity :

Human

sequence length :

273

specificity :

Reacts with MUC1

purity :

Purified

form :

PBS with 0.05% Sodium Azide

concentration :

100 ug/ml

storage stability :

Short term 4 degree C, long term aliquot and store at -20 degree C, avoid freeze thaw cycles.

tested application :

Immunohistochemistry (IHC - Paraffin; IHC - Frozen), Flow Cytometry (FC/FACS), ELISA (EIA)

app notes :

ELISA, Flo, IHC-Fr, IHC-P (1:100)

other info1 :

Target Species: Human. Immunogen Description: Deglycosylated purified MUC1 glycoprotein. Splenocytes were fused with SP2/0 mouse myeloma cells.

other info2 :

Immunogen: EMA / MUC1 antibody was raised against deglycosylated purified MUC1 glycoprotein. Splenocytes were fused with SP2/0 mouse myeloma cells.

products categories :

Family: Mucin

products description :

This gene is a member of the mucin family and encodes a membrane bound, glycosylated phosphoprotein. The protein is anchored to the apical surface of many epithelia by a transmembrane domain, with the degree of glycosylation varying with cell type. It also includes a 20 aa variable number tandem repeat (VNTR) domain, with the number of repeats varying from 20 to 120 in different individuals.

ncbi gi num :

65301117

ncbi acc num :

NP_002447.4

ncbi gb acc num :

NM_002456.5

uniprot acc num :

P15941

ncbi mol weight :

28,297 Da

ncbi pathways :

IL-7 Signaling Pathway (198857); Metabolism Of Proteins Pathway (106230); O-linked Glycosylation Pathway (1127679); O-linked Glycosylation Of Mucins Pathway (530747); Post-translational Protein Modification Pathway (161012); Termination Of O-glycan Biosynthesis Pathway (530748)

ncbi summary :

This gene encodes a membrane-bound protein that is a member of the mucin family. Mucins are O-glycosylated proteins that play an essential role in forming protective mucous barriers on epithelial surfaces. These proteins also play a role in intracellular signaling. This protein is expressed on the apical surface of epithelial cells that line the mucosal surfaces of many different tissues including lung, breast stomach and pancreas. This protein is proteolytically cleaved into alpha and beta subunits that form a heterodimeric complex. The N-terminal alpha subunit functions in cell-adhesion and the C-terminal beta subunit is involved in cell signaling. Overexpression, aberrant intracellular localization, and changes in glycosylation of this protein have been associated with carcinomas. This gene is known to contain a highly polymorphic variable number tandem repeats (VNTR) domain. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Feb 2011]

uniprot summary :

MUC1: a large cell surface glycoprotein expressed by most glandular and ductal epithelial cells and some hematopoietic cell lineages. Plays a role in adhesion and cell-cell interactions, metastasis and signaling. May provide a protective layer on epithelial surfaces. Direct or indirect interaction with actin cytoskeleton. Its cytoplasmic tail (MUC1CT) is involved in several signaling pathways, including those involving Ras, beta-catenin, p120 catenin, p53 and estrogen receptor alpha. MUC1CT also forms complexes with transcription factors, and then translocates to the nucleus by an unknown mechanism, where it is believed to influence the transcription of their target genes. MUC1CT has also been proposed to localize to mitochondrial membranes under conditions of genotoxic stress, where it attenuates the apoptotic pathway in response and confers resistance to apoptosis-inducing drugs. Aberrantly glycosylated forms expressed in human epithelial tumors, such as breast or ovarian cancer and also in non-epithelial tumor cells. Nine alternatively spliced isoforms have been described. Isoforms 5 and 9 are secreted. Isoform 7, expressed only in tumor cells, is a receptor and binds the secreted isoform 5. The binding induces the phosphorylation of the isoform 7, alters cellular morphology and initiates cell signaling. Can bind to GRB2 adapter protein. Protein type: Nuclear receptor co-regulator; Tumor suppressor; Actin-binding; Membrane protein, integral; Cell adhesion; Motility/polarity/chemotaxis. Chromosomal Location of Human Ortholog: 1q21. Cellular Component: extracellular space; cell surface; integral to plasma membrane; Golgi lumen; nuclear chromatin; cytoplasm; apical plasma membrane; vesicle. Molecular Function: protein binding; p53 binding; transcription cofactor activity. Biological Process: protein amino acid O-linked glycosylation; DNA damage response, signal transduction by p53 class mediator resulting in transcription of p21 class mediator; regulation of transcription from RNA polymerase II promoter in response to stress; cellular protein metabolic process; O-glycan processing; post-translational protein modification; DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest. Disease: Medullary Cystic Kidney Disease 1

size1 :

0.05 mg

price1 :

495 USD