Mouse Monoclonal [clone 2D2] (IgG1,k) to Human RARA / RAR Alpha | MyBioSource MBS246134 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Mouse Monoclonal [clone 2D2] (IgG1,k) to Human RARA / RAR Alpha

catalog :

MBS246134

quantity :

0.05 mg

price :

605 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

[2D2]

reactivity :

human

application :

western blot, ELISA, immunohistochemistry, enzyme immunoassay

more info or order :

MyBioSource product webpage

image

image 1 :

Anti-RARA antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

image 2 :

RARA monoclonal antibody clone 2D2 Western blot of RARA expression in HeLa NE.

image 3 :

Detection limit for recombinant GST tagged RARA is approximately 1 ng/ml as a capture antibody.

product information

catalog number :

MBS246134

products type :

Antibody

products full name :

Mouse Monoclonal [clone 2D2] (IgG1,k) to Human RARA / RAR Alpha

products short name :

[RARA / RAR Alpha]

products name syn :

[Anti-RARA / RAR Alpha Antibody (clone 2D2) IHC-plus; RARA; NR1B1; PML-RAR; RAR-alpha; RARalpha; Retinoic acid receptor alpha; Pml-rara; RAR; RAR alpha; Retinoic acid receptor; alpha; Human RARA; RAR Alpha]

other names :

[retinoic acid receptor alpha isoform 1; Retinoic acid receptor alpha; retinoic acid receptor alpha; RAR-alpha; retinoic acid receptor, alpha polypeptide; nuclear receptor subfamily 1 group B member 1; retinoic acid nuclear receptor alpha variant 1; retinoic acid nuclear receptor alpha variant 2; nucleophosmin-retinoic acid receptor alpha fusion protein NPM-RAR long form; retinoic acid receptor, alpha; Nuclear receptor subfamily 1 group B member 1]

products gene name :

[RARA]

other gene names :

[RARA; RARA; RAR; NR1B1; NR1B1; RAR-alpha]

uniprot entry name :

RARA_HUMAN

clonality :

Monoclonal

isotype :

IgG1,k

clone :

[2D2]

host :

Mouse

reactivity :

Human

sequence length :

462

specificity :

Human RARA

purity :

Protein A Purified

form :

PBS, pH 7.2

concentration :

0.4 mg/ml

storage stability :

Short term 4 degree C, long term aliquot and store at -20 degree C, avoid freeze thaw cycles.

tested application :

Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)

app notes :

ELISA, IHC-P (5 ug/ml), WB. Usage: Immunohistochemistry: Formalin-fixed paraffin-embedded sections. Sandwich ELISA: Recombinant protein. Western Blot using cell line lysates and the recombinant protein used as the immunogen.

image1 heading :

Immunohistochemistry (IHC)

image2 heading :

Western Blot (WB)

image3 heading :

ELISA (EIA)

other info1 :

Target Species: Human. Immunogen Description: RARA (NP_000955, 315 a.a. ~ 425 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

other info2 :

Immunogen Species: Immunogen: RARA/RAR Alpha antibody was raised against Human. Antigen Type: Recombinant protein. Immunogen: RARA / RAR Alpha antibody was raised against rARA (NP_000955, 315 a.a. ~ 425 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Disclaimer: Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application. Recommended Immunohistochemistry Protocol: The following protocol is a recommendation only, and MyBioSource, Inc. makes no guarantee of the results:. Tissue Preparation: . Formalin fixation and embedding in paraffin wax. Tissue Sectioning: . Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C. Deparaffinization: . Wash dry slides in 3 changes of xylene - 5 minutes each @ RT. Rehydration: . Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT. Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT. Wash slides in 1 change of 80% alcohol - 3 minutes @ RT. Rinse slides in gentle running distilled water - 5 minutes @ RT. Antigen retrieval: . Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes. Remove from heat and let stand at room temperature in buffer - 20 minutes. Rinse in 1X TBS with Tween (TBST) -1 minute @ RT. Immunostaining: . (Do not allow tissues to dry at any time during the staining procedure) . Apply a universal protein block - 20 minutes @ RT. Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT. Rinse slides in 1 X TBST - 1 minute @ RT. Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT. Rinse slides in 1X TBST -1 minute @ RT. Apply alkaline phosphatase streptavidin - 30 minutes @ RT. Rinse slides in 1X TBST -1 minute @ RT. Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT. Wash slides in distilled water - 1 minute @ RT. Dehydrate: . (This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB) . Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT. Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT. Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT. Wash slides in 3 changes of xylene - 1 minute each @ RT. Apply coverslip

products categories :

Family: NHR. Subfamily: NR1 Thyroid hormone-like

products description :

Retinoic acid receptor alpha (RAR alpha), a NR1 Thyroid Hormone-Like Receptor, has a key effect on the development of acute promyelocytic leukemia (APL).

ncbi gi num :

4506419

ncbi acc num :

NP_000955.1

ncbi gb acc num :

NM_000964.3

uniprot acc num :

P10276

ncbi mol weight :

39,700 Da

ncbi pathways :

Acute Myeloid Leukemia Pathway (83117); Acute Myeloid Leukemia Pathway (529); Adipogenesis Pathway (198832); Gene Expression Pathway (105937); Generic Transcription Pathway (105938); IL-3 Signaling Pathway (198881); Integrated Pancreatic Cancer Pathway (711360); Nuclear Receptor Transcription Pathway (105979); Nuclear Receptors Pathway (198848); Nuclear Receptors In Lipid Metabolism And Toxicity Pathway (198887)

ncbi summary :

This gene represents a nuclear retinoic acid receptor. The encoded protein, retinoic acid receptor alpha, regulates transcription in a ligand-dependent manner. This gene has been implicated in regulation of development, differentiation, apoptosis, granulopoeisis, and transcription of clock genes. Translocations between this locus and several other loci have been associated with acute promyelocytic leukemia. Alternatively spliced transcript variants have been found for this locus.[provided by RefSeq, Sep 2010]

uniprot summary :

RARA: is a receptor for retinoic acid, a potent mammalian morphogen and teratogen that has profound effects on vertebrate development. RARA is a member of the nuclear receptor superfamily. Controls cell function by directly regulating gene expression. Its phosphorylation is crucial for transcriptional activity. Aberrations involving RARA may be a cause of acute promyelocytic leukemia. Two splice-variant isoforms have been described. Protein type: DNA-binding; Nuclear receptor; Transcription factor; Oncoprotein. Chromosomal Location of Human Ortholog: 17q21. Cellular Component: nucleoplasm; cell surface; cell soma; perinuclear region of cytoplasm; nuclear chromatin; cytoplasm; dendrite; nucleus; actin cytoskeleton. Molecular Function: protein domain specific binding; protein kinase B binding; retinoic acid binding; zinc ion binding; chromatin DNA binding; translation repressor activity, nucleic acid binding; transcription coactivator activity; phosphoinositide 3-kinase regulator activity; drug binding; alpha-actinin binding; transcription factor binding; protein binding; enzyme binding; protein heterodimerization activity; protein kinase A binding; steroid hormone receptor activity; mRNA 5 -UTR binding; retinoic acid receptor activity; transcription factor activity; transcription corepressor activity; receptor binding. Biological Process: prostate gland development; negative regulation of translational initiation; estrogen receptor signaling pathway; regulation of myelination; glandular epithelial cell development; positive regulation of transcription, DNA-dependent; regulation of synaptic plasticity; female pregnancy; regulation of phosphoinositide 3-kinase activity; protein amino acid phosphorylation; response to vitamin A; germ cell development; Sertoli cell fate commitment; positive regulation of T-helper 2 cell differentiation; positive regulation of cell cycle; positive regulation of phosphoinositide 3-kinase cascade; response to ethanol; positive regulation of transcription from RNA polymerase II promoter; steroid hormone mediated signaling; negative regulation of transcription, DNA-dependent; negative regulation of apoptosis; retinoic acid receptor signaling pathway; limb development; ventricular cardiac muscle cell differentiation; negative regulation of transcription from RNA polymerase II promoter; signal transduction; response to estradiol stimulus; negative regulation of granulocyte differentiation; positive regulation of interleukin-4 production; negative regulation of cell proliferation; ureteric bud development; negative regulation of interferon-gamma production; positive regulation of cell proliferation; positive regulation of interleukin-13 production; transmembrane transport; positive regulation of interleukin-5 production; transcription initiation from RNA polymerase II promoter; response to retinoic acid; multicellular organism growth; positive regulation of binding; negative regulation of tumor necrosis factor production; liver development; embryonic camera-type eye development; positive regulation of protein kinase B signaling cascade; response to cytokine stimulus; neural tube closure; spermatogenesis; gene expression; positive regulation of neuron differentiation; apoptotic cell clearance. Disease: Acute Promyelocytic Leukemia

size1 :

0.05 mg

price1 :

605 USD

more info or order :

MyBioSource product webpage