Mouse Monoclonal [clone 2B4] (IgG2a,k) to Human TROVE2 | MyBioSource MBS245859 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Mouse Monoclonal [clone 2B4] (IgG2a,k) to Human TROVE2

catalog :

MBS245859

quantity :

0.05 mg

price :

605 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

[2B4]

reactivity :

human

application :

western blot, ELISA, immunohistochemistry, enzyme immunoassay

more info or order :

MyBioSource product webpage

image

image 1 :

Anti-TROVE2 antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.

image 2 :

TROVE2 monoclonal antibody (M01), clone 2B4. Western blot of TROVE2 expression in HeLa.

image 3 :

Detection limit for recombinant GST tagged TROVE2 is approximately 0.3 ng/ml as a capture antibody.

product information

catalog number :

MBS245859

products type :

Antibody

products full name :

Mouse Monoclonal [clone 2B4] (IgG2a,k) to Human TROVE2

products short name :

[TROVE2]

products name syn :

[TROVE2, 60 kDa ribonucleoprotein Ro, 60 kDa Ro protein, Sjoegren syndrome antigen A2, TROVE domain family member 2, Ro 60 kDa autoantigen, RORNP, SSA2, R060, SS-A, TROVE domain family, member 2]

other names :

[60 kDa SS-A/Ro ribonucleoprotein isoform 2; 60 kDa SS-A/Ro ribonucleoprotein; 60 kDa SS-A/Ro ribonucleoprotein; 60 kDa ribonucleoprotein Ro; sjoegren syndrome type A antigen; gastric cancer multi-drug resistance protein; Sjogren syndrome antigen A2 (60kD, ribonucleoprotein autoantigen SS-A/Ro); TROVE domain family, member 2; Ro 60 kDa autoantigen; Sjoegren syndrome antigen A2; Sjoegren syndrome type A antigen; SS-A; TROVE domain family member 2]

products gene name :

[TROVE2]

other gene names :

[TROVE2; TROVE2; RO60; SSA2; RORNP; RO60; SSA2; 60 kDa Ro protein; 60 kDa ribonucleoprotein Ro; RoRNP; SS-A]

uniprot entry name :

RO60_HUMAN

clonality :

Monoclonal

isotype :

IgG2a,k

clone :

[2B4]

host :

Mouse

reactivity :

Human

sequence length :

538

specificity :

Human TROVE2

purity :

Purified from ascites by Protein A

form :

PBS, pH 7.4.

concentration :

0.5 mg/ml

storage stability :

Short term 4 degree C, long term aliquot and store at -20 degree C, avoid freeze thaw cycles.

tested application :

Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)

app notes :

ELISA, IHC-P (1:100), WB (1:500 - 1:1000). Usage: Sandwich ELISA: Recombinant protein. Western Blot: Cell lysate, Recombinant protein. (Optimal dilution to be determined by the researcher)

image1 heading :

Immunohistochemistry (IHC)

image2 heading :

Western Blot (WB)

image3 heading :

ELISA (EIA)

other info1 :

Target Species: Human. Target: TROVE2. Immunogen Species: TROVE2 antibody was raised against Human. Antigen Type: Recombinant protein. Immunogen: TROVE2 antibody was raised against recombinant protein

other info2 :

Disclaimer: Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application. Recommended Immunohistochemistry Protocol: The following protocol is a recommendation only, and MyBioSource, Inc. makes no guarantee of the results:. Tissue Preparation: . Formalin fixation and embedding in paraffin wax. Tissue Sectioning: . Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C. Deparaffinization: . Wash dry slides in 3 changes of xylene - 5 minutes each @ RT. Rehydration: . Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT. Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT. Wash slides in 1 change of 80% alcohol - 3 minutes @ RT. Rinse slides in gentle running distilled water - 5 minutes @ RT. Antigen retrieval: . Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes. Remove from heat and let stand at room temperature in buffer - 20 minutes. Rinse in 1X TBS with Tween (TBST) -1 minute @ RT. Immunostaining: . (Do not allow tissues to dry at any time during the staining procedure) . Apply a universal protein block - 20 minutes @ RT. Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT. Rinse slides in 1 X TBST - 1 minute @ RT. Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT. Rinse slides in 1X TBST -1 minute @ RT. Apply alkaline phosphatase streptavidin - 30 minutes @ RT. Rinse slides in 1X TBST -1 minute @ RT. Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT. Wash slides in distilled water - 1 minute @ RT. Dehydrate: . (This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB) . Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT. Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT. Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT. Wash slides in 3 changes of xylene - 1 minute each @ RT. Apply coverslip

ncbi gi num :

31377800

ncbi acc num :

NP_004591.2

ncbi gb acc num :

NM_004600.5

uniprot acc num :

P10155

ncbi mol weight :

59,270 Da

ncbi pathways :

Systemic Lupus Erythematosus Pathway (83122); Systemic Lupus Erythematosus Pathway (534)

uniprot summary :

SSA2: RNA-binding protein that binds to several small cytoplasmic RNA molecules known as Y RNAs. May stabilize these RNAs from degradation. Belongs to the Ro 60 kDa family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: RNA-binding. Chromosomal Location of Human Ortholog: 1q31. Cellular Component: nucleoplasm; cytoplasm; ribonucleoprotein complex. Molecular Function: RNA binding; metal ion binding; U2 snRNA binding. Biological Process: transcription from RNA polymerase III promoter; immune system development; smoothened signaling pathway; response to UV. Disease: Heart Block, Congenital

size1 :

0.05 mg

price1 :

605 USD

more info or order :

MyBioSource product webpage