Mouse Monoclonal (IgG1,k) to Human CCL19 / MIP3-Beta | MyBioSource MBS245857 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Mouse Monoclonal (IgG1,k) to Human CCL19 / MIP3-Beta

catalog :

MBS245857

quantity :

0.5 mg

price :

530 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

reactivity :

human

application :

western blot, ELISA, immunohistochemistry, enzyme immunoassay

more info or order :

MyBioSource product webpage

image

image 1 :

Anti-CCL19 antibody IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 10 ug/ml.

product information

catalog number :

MBS245857

products type :

Antibody

products full name :

Mouse Monoclonal (IgG1,k) to Human CCL19 / MIP3-Beta

products short name :

[CCL19 / MIP3-Beta]

products name syn :

[Anti-CCL19 / MIP3-Beta Antibody IHC-plus; CCL19; C-C motif chemokine 19; CKb11; CC chemokine ligand 19; EBI1 ligand chemokine; EBI1-ligand chemokine; Exodus-3; ELC; MIP-3-beta; MIP3B; SCYA19; Small-inducible cytokine A19; Beta chemokine exodus-3; Beta-chemokine exodus-3; CK beta-11; MIP-3b; MIP3-Beta; Human CCL19; MIP3-Beta]

other names :

[C-C motif chemokine 19; C-C motif chemokine 19; C-C motif chemokine 19; exodus-3; CK beta-11; MIP-3-beta; EBI1 ligand chemokine; EBI1-ligand chemokine; CC chemokine ligand 19; beta chemokine exodus-3; beta-chemokine exodus-3; small-inducible cytokine A19; macrophage inflammatory protein 3 beta; macrophage inflammatory protein 3-beta; epstein-Barr virus-induced molecule 1 ligand chemokine; small inducible cytokine subfamily A (Cys-Cys), member 19; chemokine (C-C motif) ligand 19; Beta-chemokine exodus-3; CK beta-11; Epstein-Barr virus-induced molecule 1 ligand chemokine; EBI1 ligand chemokine; ELC; Macrophage inflammatory protein 3 beta; MIP-3-beta; Small-inducible cytokine A19]

products gene name :

[CCL19]

other gene names :

[CCL19; CCL19; ELC; CKb11; MIP3B; MIP-3b; SCYA19; ELC; MIP3B; SCYA19; EBI1 ligand chemokine; ELC; MIP-3-beta]

uniprot entry name :

CCL19_HUMAN

clonality :

Monoclonal

isotype :

IgG1,k

host :

Mouse

reactivity :

Human

sequence length :

specificity :

Human MIP-3beta

purity :

Protein A Purified

form :

Lyophilized from PBS

concentration :

1 mg/ml

storage stability :

Store Lyophilized at room temperature up to 1 month; Reconstituted for up to two weeks at 2-8 degree C. Aliquot and freeze at -20 degree C for long term storage. Avoid freeze/thaw cycles.

tested application :

Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)

app notes :

ELISA (2 - 4 ug/ml), IHC-P (10 ug/ml), WB (1 - 2 ug/ml). Usage: Immunohistochemistry: MBS245857 in an IHC-plus antibody, validated by our pathologists for use in IHC against formalin-fixed, paraffin-embedded human tissues (IHC validation procedure). A concentration of 10 ug/ml is recommended. ELISA: In a sandwich ELISA (assuming 100 ul/well), a concentration of 2-4 ug/ml of this antibody will detect recombinant Human MIP-3beta when used with biotinylated antigen affinity purified anti-Human MIP-3beta as the detection antibody at a concentration of approximately 0.5-1 ug/ml. Western Blot: To detect Human MIP-3beta by Western Blot analysis this antibody can be used at a concentration of 1-2 ug/ml. When used in conjunction with compatible secondary reagents the detection limit for recombinant Human MIP-3beta is 2-4 ng/lane, under reducing conditions.

image1 heading :

Immunohistochemistry (IHC)

other info1 :

Target: CCL19/MIP3-Beta. Target Species: Human. Resuspension Liquid Description: Sterile water. Immunogen Description: E.coli derived recombinant Human MIP-3beta.

other info2 :

Immunogen Species: CCL19/MIP3-Beta antibody was raised against Human. Immunogen Type: Cell extract. Immunogen: CCL19 / MIP3-Beta antibody was raised against e.coli derived recombinant Human MIP-3beta. Reconstitution: 500ul Sterile water.

products categories :

Family: Intercrine. Subfamily: Intercrine beta

products description :

May play a role not only in inflammatory and immunological responses but also in normal lymphocyte recirculation and homing. May play an important role in trafficking of T-cells in thymus, and T-cell and B-cell migration to secondary lymphoid organs. Binds to chemokine receptor CCR7. Recombinant CCL19 shows potent chemotactic activity for T-cells and B-cells but not for granulocytes and monocytes. Binds to atypical chemokine receptor ACKR4 and mediates the recruitment of beta-arrestin (ARRB1/2) to ACKR4.

ncbi gi num :

5454040

ncbi acc num :

NP_006265.1

ncbi gb acc num :

NM_006274.2

uniprot acc num :

Q99731

ncbi pathways :

Chemokine Receptors Bind Chemokines Pathway (106359); Chemokine Signaling Pathway (99051); Chemokine Signaling Pathway (96864); Class A/1 (Rhodopsin-like Receptors) Pathway (106357); Cytokine-cytokine Receptor Interaction Pathway (83051); Cytokine-cytokine Receptor Interaction Pathway (460); Defective ACTH Causes Obesity And Pro-opiomelanocortinin Deficiency (POMCD) Pathway (1127664); Disease Pathway (530764); G Alpha (i) Signalling Events Pathway (119550); GPCR Downstream Signaling Pathway (119548)

ncbi summary :

This antimicrobial gene is one of several CC cytokine genes clustered on the p-arm of chromosome 9. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The CC cytokines are proteins characterized by two adjacent cysteines. The cytokine encoded by this gene may play a role in normal lymphocyte recirculation and homing. It also plays an important role in trafficking of T cells in thymus, and in T cell and B cell migration to secondary lymphoid organs. It specifically binds to chemokine receptor CCR7. [provided by RefSeq, Sep 2014]

uniprot summary :

CCL19: May play a role not only in inflammatory and immunological responses but also in normal lymphocyte recirculation and homing. May play an important role in trafficking of T-cells in thymus, and T-cell and B-cell migration to secondary lymphoid organs. Specifically binds to chemokine receptor CCR7. Recombinant CCL19 shows potent chemotactic activity for T-cells and B-cells but not for granulocytes and monocytes. Belongs to the intercrine beta (chemokine CC) family. Protein type: Motility/polarity/chemotaxis; Secreted, signal peptide; Secreted. Chromosomal Location of Human Ortholog: 9p13. Cellular Component: extracellular space; extracellular region. Molecular Function: CCR chemokine receptor binding; chemokine activity; chemokine receptor binding; CCR10 chemokine receptor binding; CCR7 chemokine receptor binding. Biological Process: formation of immunological synapse; positive regulation of interleukin-12 production; positive regulation of dendritic cell antigen processing and presentation; cell maturation; positive regulation of JNK cascade; positive regulation of NF-kappaB import into nucleus; positive regulation of endocytosis; positive regulation of interleukin-1 beta secretion; activation of JNK activity; positive regulation of receptor-mediated endocytosis; dendritic cell chemotaxis; establishment of T cell polarity; positive regulation of T cell proliferation; cell communication; inflammatory response; positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of chemotaxis; response to virus; positive regulation of phosphoinositide 3-kinase activity; positive regulation of tumor necrosis factor production; cellular calcium ion homeostasis; positive regulation of protein kinase B signaling cascade; myeloid dendritic cell chemotaxis; positive regulation of protein kinase activity; release of sequestered calcium ion into cytosol; T cell costimulation; immune response; positive regulation of T-helper 1 cell differentiation

size1 :

0.5 mg

price1 :

530 USD

more info or order :

MyBioSource product webpage