Mouse Monoclonal [clone 3B3] (IgG1) to Human ACTA1 / ASMA | MyBioSource MBS245785 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Mouse Monoclonal [clone 3B3] (IgG1) to Human ACTA1 / ASMA

catalog :

MBS245785

quantity :

0.05 mg

price :

495 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

3B3

reactivity :

human, rat

application :

western blot, ELISA, immunohistochemistry, enzyme immunoassay

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS245785

products type :

Antibody

products full name :

Mouse Monoclonal [clone 3B3] (IgG1) to Human ACTA1 / ASMA

products short name :

ACTA1 / ASMA

products name syn :

Anti-ACTA1 / ASMA Antibody (clone 3B3) IHC-plus; ACTA1; ACTA; Actin; alpha skeletal muscle; Alpha-actin-1; CFTD; CFTD1; CFTDM; MPFD; NEM1; NEM2; ASMA; NEM3; Human ACTA1; ASMA

other names :

actin, alpha skeletal muscle; Actin, alpha skeletal muscle; actin, alpha skeletal muscle; nemaline myopathy type 3; actin, alpha 1, skeletal muscle; Alpha-actin-1

products gene name :

ACTA1

products gene name syn :

ASMA

other gene names :

ACTA1; ACTA1; ACTA; ASMA; CFTD; MPFD; NEM1; NEM2; NEM3; CFTD1; CFTDM; ACTA

uniprot entry name :

ACTS_HUMAN

clonality :

Monoclonal

isotype :

IgG1

clone :

3B3

host :

Mouse

reactivity :

Goat, Human, Pig, Rabbit, Rat

sequence length :

377

specificity :

Highly specific for alpha- skeletal actin, and does not cross react with other actin isoforms. The epitope recognized by 3B3 is highly conserved. Therefore the antibody cross-reacts with many other species.

purity :

Purified

form :

PBS containing 0.09% sodium azide

concentration :

1 mg/ml

storage stability :

Long term: -20 degree C; Short term: +4 degree C. Avoid repeat freeze-thaw cycles.

tested application :

Immunohistochemistry (IHC - Paraffin; IHC - Frozen), Western Blot (WB), ELISA (EIA)

app notes :

ELISA, IHC-Fr (1:100 - 1:1000), IHC-P (10 ug/ml), WB (1:1000 - 1:5000)

other info1 :

Target Species: Human. Immunogen Description: Peptide comprising the N-terminal nonapeptide of alpha-skeletal actin with an acetylated N-terminus (Ac-DEDETTALVC-COOH) coupled to keyhole limpet hemocyanin through the cysteine residue

products description :

Actin alpha 1 which is expressed in skeletal muscle is one of six different actin isoforms which have been identified. Actins are highly conserved proteins that are involved in cell motility, structure and integrity. Alpha actins are a major constituent of the contractile apparatus. The product encoded by this gene belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity.

ncbi gi num :

4501881

ncbi acc num :

NP_001091.1

ncbi gb acc num :

NM_001100.3

uniprot acc num :

P68133

ncbi mol weight :

42,051 Da

ncbi pathways :

Caspase Cascade In Apoptosis Pathway (137974); Hypothetical Network For Drug Addiction Pathway (198776); Myometrial Relaxation And Contraction Pathways (198759); RhoA Signaling Pathway (169356); Signaling Events Mediated By Focal Adhesion Kinase Pathway (169348); Striated Muscle Contraction Pathway (198903)

ncbi summary :

The product encoded by this gene belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actins being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. This actin is an alpha actin that is found in skeletal muscle. Mutations in this gene cause nemaline myopathy type 3, congenital myopathy with excess of thin myofilaments, congenital myopathy with cores, and congenital myopathy with fiber-type disproportion, diseases that lead to muscle fiber defects. [provided by RefSeq, Jul 2008]

uniprot summary :

ACTA1: Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to 4 others. Interacts with TTID. Interacts (via its C-terminus) with USP25; the interaction occurs for all USP25 isoforms but is strongest for isoform USP25m in muscle differentiating cells. Belongs to the actin family. Protein type: Cell development/differentiation; Cytoskeletal; Contractile; Motility/polarity/chemotaxis. Chromosomal Location of Human Ortholog: 1q42.13. Cellular Component: extracellular space; sarcomere; stress fiber; actin filament; cytosol; striated muscle thin filament; actin cytoskeleton. Molecular Function: protein binding; myosin binding; structural constituent of cytoskeleton; ADP binding; ATP binding. Biological Process: skeletal muscle fiber adaptation; muscle contraction; muscle thin filament assembly; response to steroid hormone stimulus; response to mechanical stimulus; response to extracellular stimulus; response to lithium ion; skeletal muscle fiber development; cell growth; muscle filament sliding. Disease: Nemaline Myopathy 3; Myopathy, Congenital, With Fiber-type Disproportion

size1 :

0.05 mg

price1 :

495 USD

more info or order :

MyBioSource product webpage