antibody

Mouse Monoclonal (IgG1) to Human MLH1

MBS245337

0.05 mL

530 USD

monoclonal

mouse

nonconjugated

[4C9C7]

western blot, ELISA, immunohistochemistry, immunocytochemistry, enzyme immunoassay, immunohistochemistry - paraffin section

Antibody

Mouse Monoclonal (IgG1) to Human MLH1

[MLH1]

[Anti-MLH1 Antibody IHC-plus; MLH1; COCA2; FCC2; HMLH1; HNPCC; HNPCC2; MutL protein homolog 1; Human MLH1]

[DNA mismatch repair protein Mlh1 isoform 1; DNA mismatch repair protein Mlh1; DNA mismatch repair protein Mlh1; mutL homolog 1, colon cancer, nonpolyposis type 2; mutL homolog 1; MutL protein homolog 1]

[MLH1]

[MLH1; MLH1; FCC2; COCA2; HNPCC; hMLH1; HNPCC2; COCA2]

MLH1_HUMAN

Monoclonal

IgG1

[4C9C7]

mouse

Human

756

Human, Monkey

Ascites

Ascitic fluid, 0.03% sodium azide.

Short term: 4°C; Long term: -20°C

Immunohistochemistry (IHC),Immunohistochemistry-Paraffin (IHC-P), Immunofluorescence (IF), Western Blot (WB), ELISA (EIA)

Immunohistochemistry-Paraffin (IHC-P) 1:200. Immunofluorescence (IF) 1:200-1000. Western Blot (WB) 1:100-1:500. ELISA (EIA) 1:1000. Optimal dilution to be determined by the researcher. Immunohistochemistry : MBS245337 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibdody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specifity. The optimal working concentration for MBS245337 was determined to be 1:200.

Immunohistochemistry (IHC)

Target Species: MLH1. Immunogen Species: MLH1 antibody was raised against Human. Immunogen: MLH1 antibody was raised against purified recombinant fragment of MLH1 (aa381-483) expressed in E Coli.

Disclaimer: Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application. Recommended Immunohistochemistry Protocol: The following protocol is a recommendation only, and MyBioSource, Inc. makes no guarantee of the results:. Tissue Preparation: . Formalin fixation and embedding in paraffin wax. Tissue Sectioning: . Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C. Deparaffinization: . Wash dry slides in 3 changes of xylene - 5 minutes each @ RT. Rehydration: . Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT. Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT. Wash slides in 1 change of 80% alcohol - 3 minutes @ RT. Rinse slides in gentle running distilled water - 5 minutes @ RT. Antigen retrieval: . Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes. Remove from heat and let stand at room temperature in buffer - 20 minutes. Rinse in 1X TBS with Tween (TBST) -1 minute @ RT. Immunostaining: . (Do not allow tissues to dry at any time during the staining procedure) . Apply a universal protein block - 20 minutes @ RT. Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT. Rinse slides in 1 X TBST - 1 minute @ RT. Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT. Rinse slides in 1X TBST -1 minute @ RT. Apply alkaline phosphatase streptavidin - 30 minutes @ RT. Rinse slides in 1X TBST -1 minute @ RT. Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT. Wash slides in distilled water - 1 minute @ RT. Dehydrate: . (This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB) . Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT. Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT. Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT. Wash slides in 3 changes of xylene - 1 minute each @ RT. Apply coverslip. Note: During shipment,small volumes of product will ocassionally become entrapped in the seal of the product vial. We recommend briefly centrifuging the vial to dislodge any liquid in the container's cap prior to opening. Warning: This reagent may contain sodium azide. The chemical, physical, and toxicologial properties of this material have not been thoughly investigated.Standard Laboratory Practices should be followed. Avoid skin and eye contact,inhalation, and ingestion. Sodium azide forms hydrazoic acid under acidic conditions and may react with lead or copper plubing to form highly explosive metal azides. On disposal, flush with large volumes of water to prevent accumulation.

MLH1 was identified as a locus frequently mutated in hereditary nonpolyposis colon cancer (HNPCC). When cloned, it was discovered to be a human homolog of the E Coli mismatch repair gene mutS, consistent with the characteristic alterations in microsatellite sequences (RER+ phenotype) found in HNPCC.

4557757

NP_000240.1

NM_000249.3

P40692

BRCA1-associated Genome Surveillance Complex (BASC) Pathway (413428); BRCA1-associated Genome Surveillance Complex (BASC) Pathway (890555); Cell Cycle Pathway (530733); Colorectal Cancer Pathway (83106); Colorectal Cancer Pathway (518); DNA Repair Pathway (105837); Direct P53 Effectors Pathway (137939); Endometrial Cancer Pathway (83109); Endometrial Cancer Pathway (521); Fanconi Anemia Pathway (377262)

This gene was identified as a locus frequently mutated in hereditary nonpolyposis colon cancer (HNPCC). It is a human homolog of the E. coli DNA mismatch repair gene mutL, consistent with the characteristic alterations in microsatellite sequences (RER+phenotype) found in HNPCC. Alternative splicing results in multiple transcript variants encoding distinct isoforms. Additional transcript variants have been described, but their full-length natures have not been determined.[provided by RefSeq, Nov 2009]

MLH1: a protein involved in the repair of mismatches in DNA. Binds PMS2 or MLH1 and MLH3. Part of the BRCA1-associated genome surveillance complex (BASC), which contains BRCA1, MSH2, MSH6, MLH1, ATM, BLM, PMS2 and the RAD50- MRE11-NBS1 protein complex. This association could be a dynamic process changing throughout the cell cycle and within subnuclear domains. Interacts with MBD4. Interacts with EXO1. Defects in MLH1 are the cause of hereditary non-polyposis colorectal cancer type 2; Turcot syndrome, an autosomal dominant disorder characterized by malignant tumors of the brain; Muir-Torre syndrome (MTS), a rare autosomal dominant disorder characterized by sebaceous neoplasms and visceral malignancy; lobular carcinoma in situ; endometrial cancer; and non-polyposis colorectal cancer. Protein type: DNA repair, damage; Tumor suppressor. Chromosomal Location of Human Ortholog: 3p21.3. Cellular Component: nucleoplasm; MutLalpha complex; male germ cell nucleus; chiasma; membrane; synaptonemal complex; nucleus. Molecular Function: protein binding; ATPase activity; guanine/thymine mispair binding; MutSalpha complex binding; single-stranded DNA binding; ATP binding. Biological Process: oogenesis; mismatch repair; somatic hypermutation of immunoglobulin genes; negative regulation of mitotic recombination; isotype switching; spindle midzone assembly involved in meiosis; DNA repair; double-strand break repair via nonhomologous end joining; poly(A) tail shortening; meiotic metaphase I plate congression; DNA damage response, signal transduction resulting in induction of apoptosis; synapsis; male meiosis chromosome segregation; resolution of meiotic joint molecules as recombinants; spermatogenesis. Disease: Muir-torre Syndrome; Mismatch Repair Cancer Syndrome; Colorectal Cancer, Hereditary Nonpolyposis, Type 2

0.05 mL

530 USD